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EC number: 230-989-6 | CAS number: 7394-38-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 September 2001 - 18 October 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Non-GLP but documented in detail. Further details on test item not reported.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- yes
- Remarks:
- The test procedure was conducted as described in detail in BMG's Standard Operating Procedure (Standardarbeitsanweisung BMG-1028).
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- yes
- Remarks:
- The test procedure was conducted as described in detail in BMG's Standard Operating Procedure (Standardarbeitsanweisung BMG-1028).
- Principles of method if other than guideline:
- Alteration of the standard procedure: At the end of the test the pH value of both inoculum blanks and the procedure control was 7.2 and 6.6, respectively. The pH value of both test suspensions was 6.5, respectively.
- GLP compliance:
- no
- Remarks:
- The study was conducted in accordance with SN EN 45001
- Specific details on test material used for the study:
- The test material was a 65 % solution in water.
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from the aeration tank of a municipal biological waste water treatment plant, ARA Werdhölzli, CH-8048, Zürich,19 September 2001; 9:30 a.m.
- Storage length: No storage. Used immediately after sampling
- Pretreatment: not adapted, not pre-conditioned
- Concentration of sludge: 30 mg/l dry matter in the final mixture
- Test Medium: Aerobic mineral salts medium prepared with deionised water (details on medium available in report) - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Remarks:
- Nominal
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral salts medium. See table on Any other information on materials and methods section
- Additional substrate: none
- Solubilising agent: none
- Test temperature: 22 +/- 0.5 °C
- pH: 7.4 +/- 0.2
- pH adjusted: yes, with NaOH or H2SO4, if necessary.
- Aeration of dilution water: no
- Continuous darkness: yes
TEST SYSTEM
- Test units: 1200 ml closed glass bottle (tightly closed with a butyl rubber stopper) containing a total volume of test solution of 400 ml
- Number of culture flasks: 2 flasks with test suspension
- Test vessels were agitated on an orbital shaker (100 r.p.m.)
SAMPLING
- Sampling frequency: after 1 day and then at regular intervals of 4 days
- Sampling method: 0.2 ml headspace samplig with gas syringe
- Sample storage before analysis: no, direct injection to GC
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 replicates containing inoculum and test medium
- Abiotic sterile control: 1 replicate containing test substance, test medium and 0.2 mM HgCl as sterilizing agent
- Toxicity control: 1 replicate inoculum + test medium + test substance + sodium benzoate as ready biodegradable reference compound
- Procedure control: 1 replicate containing inoculum, test medium and sodium benzoate as ready biodegradable reference compound
STATISTICAL METHODS:
Values of % degradation were calculated for each sample from the test suspension and the procedure control. The arithmetic mean of % degradation in each test flask was calculated. - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- The positive control, sodium benzoate, reached 90 % biodegradation after 14 days, thus confirming suitability of inoculum and test conditions.
The ThOD of the reference compound sodium benzoate was calculated to be 1.665 mg O2/mg substance. - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 90
- Sampling time:
- 28 d
- Remarks on result:
- other: mean of two replicate flasks
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 80
- Sampling time:
- 11 d
- Remarks on result:
- other: mean of two replicate flasks
- Details on results:
- Significant biodegradation of the test substance was observed after a lag phase of about 1 day.
The respective DOC concentrations at the beginning and at the end of the test (after 28 days) were (in mg/l):
31.8 and 1.13 for the test units (mean of two replicates)
0.30 and 1.18 for the inoculum blank (mean of two replicates)
57.5 and 1.46 for the procedure control (one replicate)
The calculated biodegradation based on DOC measurement reached 99 % for REVATOL NS and for sodium benzoate. The data show that the consumed O2, was used for complete mineralization of the test compound, since the determined degradation values based on BOD were within the same range than those based on DOC. - Validity criteria fulfilled:
- not specified
- Remarks:
- The positive control, sodium benzoate, reached 90% biodegradation after 14 days, thus confirming suitability of inoculum and test conditions.
- Interpretation of results:
- readily biodegradable
- Conclusions:
- In this study according to OECD 301 F, the test material reached a biodegradation of 90%. It reached the pass level of 60 % for ready biodegradability in the Manometric Respirometry Test within the 10-d window and, therefore, can be termed as readily biodegradable.
- Executive summary:
The biodegradability of the test material exposed to microorganisms derived from activated sludge of a municipal sewage treatment plant was investigated under aerobic static exposure conditions.
The biodegradability of the test material based on O2 consumption was calculated to be 90 % after 28 days as compared to the theoretical O2 demand (ThOD).
The biodegradation of the test material reached 80 % at the end of the 10-d window.
Significant biodegradation of the test substance was observed after a lag phase of about 1 day.
The procedure control sodium benzoate reached 90 % biodegradation after 14 days, thus confirming suitability of inoculum and test conditions.
The calculated biodegradation based on DOC measurement reached 100 % for the test material and for sodium benzoate. The data show that the consumed O2 was used for complete mineralization of the test compound, since the determined degradation values based on BOD were within the same range than those based on DOC.
the test material reached the pass level of 60 % for ready biodegradability in the Manometric Respirometry Test within the 10-d window and, therefore, can be termed as readily biodegradable.
Reference
Table: O2 concentration (mg O2 per test sample) of the test units, the procedure, toxicity and the abiotic sterile control and the inoculum blank.
Time (days) | Test Unit N1 | Test unit N2 | Toxicity control | Abiotic sterile control | Inoculim blank (mean of 2 replicates) |
Procedure control |
0 | 244.0 | 244.0 | n.d. | n.d. | 244.0 | 244.0 |
4 | 241.7 | 241.0 | n.d. | n.d. | 242.2 | 225.9 |
7 | 211 .3 | 206.8 | n.d. | n.d. | 235.4 | 185.6 |
11 | 199.5 | 204.4 | n.d. | n.d. | 237.7 | 180.6 |
14 | 197.2 | 201.0 | n.d. | n.d. | 235.2 | 175.3 |
18 | 194.1 | 196.0 | n.d. | n.d. | 234.6 | 174.2 |
21 | 194.9 | 193.0 | n.d. | n.d. | 233.5 | 173.2 |
25 | 194.3 | 191.6 | n.d. | n.d. | 232.3 | 171.5 |
28 | 191 .8 | 188.8 | n.d. | n.d. | 230.4 | 169.6 |
Description of key information
A study according to OECD 301F with the substance (65 % solution in water) was available. It is a non-GLP study but documented in detail. Further details on the test item were not reported. The study is rated Klimisch 2.
The biodegradability of the test material based on O2 consumption was calculated to be 90 % after 28 days as compared to the theoretical O2 demand (ThOD).
The biodegradation of the test material reached 80 % at the end of the 10-d window.
The calculated biodegradation based on DOC measurement reached 100 % for the test material and for sodium benzoate. The data show that the consumed O2 was used for complete mineralization of the test compound, since the determined degradation values based on BOD were within the same range than those based on DOC.
The active ingredient of the test material is p-Nitrobenzoic acid, compound with 2,2',2''-nitrilotriethanol (1:1).
Therefore the results with the test material are applicable to the substance p-Nitrobenzoic acid, compound with 2,2',2''-nitrilotriethanol (1:1) CAS no. 7394-38-9.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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