Registration Dossier
Registration Dossier
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EC number: 226-408-0 | CAS number: 5395-50-6
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Tetrahydro-1,3,4,6-tetrakis(hydroxymethyl)imidazo[4,5-d]imidazole-2,5(1H,3H)-dione
- EC Number:
- 226-408-0
- EC Name:
- Tetrahydro-1,3,4,6-tetrakis(hydroxymethyl)imidazo[4,5-d]imidazole-2,5(1H,3H)-dione
- Cas Number:
- 5395-50-6
- Molecular formula:
- C8H14N4O6
- IUPAC Name:
- 1,3,4,6-tetrakis(hydroxymethyl)-octahydro-[1,3]diazolo[4,5-d]imidazole-2,5-dione
1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Origin
Sammlung von Algenkulturen (SAG),
Pflanzenphysiologisches Institut der Universität Göttingen,
Nikolausberger Weg 18, D-37073 Göttingen
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 21.9-23.1°C
- pH:
- 7.7 - 8.7
- Nominal and measured concentrations:
- Nominal
7 concentration leveis in a geometrical series with a factor of 2
The concentrations of Formaldehyde <23.1 % wlw content in the test item) were analysed at all
concentrations at test start and test end. The concentrations of the active ingredient
Tetramethylolacetylene diurea (4-ADH) were calculated based on the results of the formaledhyde
(HCHO) determinations. The recovery rates at the test start were in the range of 77 - 83 %
whereas at the end of the test Formaldehyde only could be detected in the two highest dosage
leveis with recovery rates between 4 and 9 %, respectively - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.85 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- 4-ADH
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.22 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- 4-ADH
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- ErC50 (72h) = 0.94 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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