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EC number: 220-293-0 | CAS number: 2706-28-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03.10.2017 - 06.10.2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Experimental test result performed using standard test guideline
- Justification for type of information:
- Data is from ABITEC lab report.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Principles of method if other than guideline:
- Aim of this study was to evaluate the nature of chemical when comes in contact with the test organism. Test was conducted according to the OECD guideline 201.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): C.I. Acid Yellow 9
- Molecular formula: C12H11N3O6S2.2Na
- Molecular weight : 401.3301g/mol
- Substance type:Organic
- Physical state:Solid - Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Details on test solutions:
- The test solution 100 mg/l was prepared by dissolving yellow powder in OECD growth medium.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name:
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available
ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed: - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 23±2°C
- pH:
- Test at highest concentration: 8 (Change to 7.8 during tests)
Control: 8.0 (changed to 7.8 during test) - Nominal and measured concentrations:
- 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 15ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: IC1.6
- Remarks:
- %
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Only 1.6 % inhibition was observed after 72 hrs.
- Results with reference substance (positive control):
- - Results with reference substance valid
- EC50: 0.77 mg/L (24 hours) - Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of chemical disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate (C.I. Acid Yellow 9), the inhibitory concentration was 100 mg/l on that only 1.6 % inhibition was observed after the 72hrs incubation period.
- Executive summary:
Aim of this study was to evaluate the nature of chemical disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate (C.I. Acid Yellow 9) when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The test solution 100 mg/l was prepared by dissolving yellow powder in OECD growth medium. Effects on the growth rate of the organism were studied.
Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of chemical disodium 2-amino-5-[(4-sulfonatophenyl) diazenyl]benzenesulfonate (C.I. Acid Yellow 9), the inhibitory concentration was 100 mg/l on that only 1.6 % inhibition was observed after the 72hrs incubation period. This value indicates that the substance is likely to be non-hazardous to aquatic algae and cannot be classified as per the CLP classification criteria.
Reference
Description of key information
Aim of this study was to evaluate the nature of chemical disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate (C.I. Acid Yellow 9) when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The test solution 100 mg/l was prepared by dissolving yellow powder in OECD growth medium. Effects on the growth rate of the organism were studied. Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of chemical disodium 2-amino-5-[(4-sulfonatophenyl) diazenyl]benzenesulfonate (C.I. Acid Yellow 9), the inhibitory concentration was 100 mg/l on that only 1.6 % inhibition was observed after the 72hrs incubation period. This value indicates that the substance is likely to be non-hazardous to aquatic algae and cannot be classified as per the CLP classification criteria.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
Additional information
Summarized result for the toxicity of chemical disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate on the growth of aquatic algae which are as follows:
In the first key study for the target chemical disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate (2706 -28 -7) from ABITEC report 2017, toxicity was measured.
Aim of this study was to evaluate the nature of chemical disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate (C.I. Acid Yellow 9) when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The test solution 100 mg/l was prepared by dissolving yellow powder in OECD growth medium. Effects on the growth rate of the organism were studied. Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of chemical disodium 2-amino-5-[(4-sulfonatophenyl) diazenyl]benzenesulfonate (C.I. Acid Yellow 9), the inhibitory concentration was 100 mg/l on that only 1.6 % inhibition was observed after the 72hrs incubation period. This value indicates that the substance is likely to be non-hazardous to aquatic algae and cannot be classified as per the CLP classification criteria.
Similarly first study was supported by the second supporting study from the UERL report for the target chemical disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate (2706 -28 -7).
The effect of test item disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate, (CAS No. 2706-28-7) was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25mg/L, 12.5mg/L, 25mg/L, 50mg/L, 100mg/L and 200mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC25 calculated graphically through probit analysis was observed to be 239.918mg/L and the EC50 value was determined to be > 200 mg/l, respectively. Thus, based on the EC50 value, test substance can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.
Based on the above studies chemical was consider as nontoxic and not classified as per the CLP classification criteria.
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