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EC number: 216-323-7 | CAS number: 1559-35-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 24, 2017 to June 16, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: TXEG
- Expiration date of the lot/batch: December 13, 2017
- Purity test date: December 13, 2016
RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity:
- Specific activity:
- Locations of the label:
- Expiration date of radiochemical substance:
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: 20 +/- 5 degrees Celsius in the dark
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:
FORM AS APPLIED IN THE TEST (if different from that of starting material)
TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)
OTHER SPECIFICS: - Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- The test medium of the highest test concentration of nominal 100 mg test item/L was prepared by dissolving 106.0 mg test item into1060 mL test water by intense stirring for 10 minutes. Adequate volumes of this test medium were diluted with test water to prepare the test media of the other desired test concentrations. The test media were prepared just before introduction of the daphnids (= start of the test).
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: in-house cultures
- Feeding during test: none
- Food type: green algae, Desmodesmus subspicatus
- Amount:
- Frequency: at least five days/week
ACCLIMATION
- Acclimation period: none
- Acclimation conditions (same as test or not): same
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.5 mmol/L (250 mg/L) CaCO3
- Test temperature:
- 19.8 to 20.5 degrees Celsius
- pH:
- 7.9 to 8.4
- Dissolved oxygen:
- 8.7 to 9.0 mg/L
- Salinity:
- not applicable
- Conductivity:
- < 5 uS/cm
- Nominal and measured concentrations:
- Nominal concentrations: 100, 45, 30.7, 9.4, and 4.3 mg/L and a control
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass beakers
- Type (delete if not applicable): open but covered with a lid
- Material, size, headspace, fill volume: 60 mL
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter): static
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): none
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water - Elendt M4
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Salinity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
VEHICLE CONTROL PERFORMED: yes/no
RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 45.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities:
- Observations on body length and weight: no
- Other biological observations: immobility
- Mortality of control: 0
- Other adverse effects control: none
- Abnormal responses: none - Reported statistics and error estimates:
- The 24-hour and 48-hour EC50, EC20 and EC10 and the 95 % confidence limits were calculated by probit analysis. The NOEC and LOEC after 24 and 48 hours were determined directly from the raw data. The software used to perform the statistical analysis was ToxRat Professional, Version 3.2.1, ToxRat Solutions GmbH.
- Validity criteria fulfilled:
- yes
- Conclusions:
- No control mortality was observed. The 48-hr EC50 value was determined to be 45.5 mg/L with values for the NOEC and LOEC of 20.7 and 45.5 mg/L respectively.
- Executive summary:
The toxic effect of the test item Eastman (TM) EEH Solvent to Daphnia magna was assessed in a static concentration-response test. The 48-hour NOEC was determined to be 20.7 mg test item/L. The 48-hour LOEC was determined to be 45.5 mg test item/L and the 48-hour EC50 value was calculated to be 45.50 mg test item/L. The quantification of the test item Eastman (TM) EEH Solvent in the test samples gas chromatography with flame ionization detection. At the start of the test 109% of the nominal test concentrations were found (average of the nominal concentrations of 100, 45.5 and 20.7 mg test item/L). After 48 hours test duration, 103% of the nominal value was determined (average of the nominal concentrations of 100, 45.5 and 20.7 mg test item/L). During the test the daphnids were exposed to a mean of 106% of nominal (average of the nominal concentrations of 100, 45.5 and 20.7 mg test item/L). The nominal concentrations of 9.4 and 4.3 mg test item/L were below the Limit of Quantification (LOQ). As these concentrations were below the 48-hours NOEC (see Table 1), dose verification was not required according to the study plan. Since the nominal concentrations of 100, 45.5 and 20.7 mg test item/L were within 100±20% recovery at test start and test end, all biological results were calculated based on nominal concentrations.
Reference
Description of key information
The toxic effect of the test item Eastman (TM) EEH Solvent to Daphnia magna was assessed in a static concentration-response test. The 48-hour NOEC was determined to be 20.7 mg test item/L. The 48 -hour LOEC was determined to be 45.5 mg test item/L and the 48-hour EC50 value was calculated to be 45.50 mg test item/L. All test concentrations were based on nominmal concentrations.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 45.5 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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