2,2'-(vinylenedi-p-phenylene)bisbenzoxazole

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data of read across chemicals

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: As mention in below principle

Principles of method if other than guideline:

WoE report is prepared based on toxicity to microorganisms study:
1 and 2nd

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Photobacterium phosphoreum

Details on inoculum:

1 and 2nd study: Species: Photobacterium phosphoreum commonly known as Vibrio fischerii
Strain: NRRL-B-11177

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

30 min

Post exposure observation period:

5, 15 and 30 minutes

Test temperature:

15°C

pH:

5-9

Nominal and measured concentrations:

Measured

Reference substance (positive control):

yes

Remarks:

Most frequently used and recommended reference chemicals are phenol and sodium pentachlorophenate

Key result

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

3.35 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: toxicity to luminescent bacteria

Remarks on result:

other: 1st study

Key result

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

5.43 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: toxicity to luminescent bacteria

Remarks on result:

other: 2nd study

1. study

- pT -Measured concentrations (in log(L/mmol) at which there is a 50% reduction of the light emission relative to controls at the stated exposure times of 5, 15 and 30 min

- ppm -The compound's toxicity expressed in parts per million (ppm) or mg/L.

 

Name	5 min- pT	15 min- pT	30 min-pT	ppm
Test chemical	1.76	1.86	1.89	3.35

2. study:

- pT -Measured concentrations (in log(L/mmol) at which there is a 50% reduction of the light emission relative to controls at the stated exposure times of 5, 15 and 30 min

- ppm -The compound's toxicity expressed in parts per million (ppm) or mg/L.

 

Name	5 min- pT	15 min- pT	30 min-pT	ppm
Test chemical	1.54	1.67	1.71	5.43

Validity criteria fulfilled:

not specified

Conclusions:

1. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 3.35 mg/l after 30 minutes exposure with test chemical.
2. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 5.43 mg/l and 6.83 mg/l after 30 minutes exposure with test chemical.
Thus based on the overall studies, it was concluded that the test chemical was toxic and the toxic concentration ranges from 3.35 mg/l to 6.83 mg/l.

Executive summary:

Various studies available for the test chemical were reviewed to determine the toxic nature of test material on the growth of microorganisms. The studies are as mentioned below:

The comparatively quick and low cost bioassay with Photobacterium phosphoreum, strain NRRL-B-11177 to access the toxicity of test chemical to aquatic bacteria. The luminescent bacterial bioassay requires only a short period of time, i.e. minutes. In terms of testing a broad range of different chemicals with many different modes of action, a somewhat longer assay time, i.e. 30 min, is preferred, mainly to ascertain that potential diffusion problems are eliminated at source. Normally the pT values for a particular chemical rarely vary much with exposure time. The most commonly used operating conditions are 15°C for the EC50 tests with exposure times of 5 to 30 min. The pH of the test system can vary in the range of 5< pH <9 without great effect on the luminescence. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 3.35 mg/l after 30 minutes exposure with test chemical.

Similarly in the second study the comparatively quick and low cost bioassay with Photobacterium phosphoreum, strain NRRL-B-11177 to access the toxicity of test chemical to aquatic bacteria. The luminescent bacterial bioassay requires only a short period of time, i.e. minutes. In terms of testing a broad range of different chemicals with many different modes of action, a somewhat longer assay time, i.e. 30 min, is preferred, mainly to ascertain that potential diffusion problems are eliminated at source. Normally the pT values for a particular chemical rarely vary much with exposure time. The most commonly used operating conditions are 15°C for the EC50 tests with exposure times of 5 to 30 min. The pH of the test system can vary in the range of 5< pH <9 without great effect on the luminescence. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 5.43 mg/l and 6.83 mg/l after 30 minutes exposure with test chemical.

Thus based on the overall studies, it was concluded that the test chemical was toxic and the toxic concentration ranges from 3.35 mg/l to 6.83 mg/l.

Description of key information

1. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 3.35 mg/l after 30 minutes exposure with test chemical.

2. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 5.43 mg/l and 6.83 mg/l after 30 minutes exposure with test chemical.

Thus based on the overall studies, it was concluded that the test chemical was toxic and the toxic concentration ranges from 3.35 mg/l to 6.83 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

6.83 mg/L

Additional information

Various studies available for the test chemical were reviewed to determine the toxic nature of 2,2'-(ethene-1,2-diyldi-4,1-phenylene)bis(1,3-benzoxazole) (CAS no. 1533 -45 -5) on the growth of microorganisms. The studies are as mentioned below:

 

The comparatively quick and low cost bioassay with Photobacterium phosphoreum, strain NRRL-B-11177 to access the toxicity of test chemical to aquatic bacteria. The luminescent bacterial bioassay requires only a short period of time, i.e. minutes. In terms of testing a broad range of different chemicals with many different modes of action, a somewhat longer assay time, i.e. 30 min, is preferred, mainly to ascertain that potential diffusion problems are eliminated at source. Normally the pT values for a particular chemical rarely vary much with exposure time. The most commonly used operating conditions are 15°C for the EC50 tests with exposure times of 5 to 30 min. The pH of the test system can vary in the range of 5< pH <9 without great effect on the luminescence. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 3.35 mg/l after 30 minutes exposure with test chemical.

 

 

Similarly in the second study the comparatively quick and low cost bioassay with Photobacterium phosphoreum, strain NRRL-B-11177 to access the toxicity of test chemical to aquatic bacteria. The luminescent bacterial bioassay requires only a short period of time, i.e. minutes. In terms of testing a broad range of different chemicals with many different modes of action, a somewhat longer assay time, i.e. 30 min, is preferred, mainly to ascertain that potential diffusion problems are eliminated at source. Normally the pT values for a particular chemical rarely vary much with exposure time. The most commonly used operating conditions are 15°C for the EC50 tests with exposure times of 5 to 30 min. The pH of the test system can vary in the range of 5< pH <9 without great effect on the luminescence. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 5.43 mg/l and 6.83 mg/l after 30 minutes exposure with test chemical.

 

Thus based on the overall studies, it was concluded that the test chemical was toxic and the toxic concentration ranges from 3.35 mg/l to 6.83 mg/l.