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EC number: 210-502-3 | CAS number: 617-04-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2021-06-29 to 2021-08-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 2019
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentration: 100 mg/L (limit test)
- Sampling method: The Limit concentration and the control were analytically verified in
the fresh media at the start of exposure and at the renewal of the test solutions (0 and 72 hours) as well as in the 24-hours old media at the renewal and at the end of the exposure (24 and 96 hours).
- Sample storage conditions before analysis: All samples were stored at room temperature until the start of the analysis, if necessary. Prepared samples were stored in the autosampler at room temperature until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Limit test: 100 mg test item/L. An appropriate amount of the test item was weighted out and transferred into a test vessel filled with an appropriate amount of the dilution water. The test media was mixed with an ultraturrax (1 min, 17000 rpm). This procedure was repeated for every water renewal.
- Controls: Dilution water without test item
- Evidence of undissolved material: none - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Source: Umweltbundesamt, Schichauweg 58, D-12307 Berlin, Germany, brood stock maintained at test facility
- Age at study initiation: juvenile
- Average length at study end: 1.79 cm
- Average weight at study end: 0.067 g
ACCLIMATION
- Acclimation period: at least 9 days
- Acclimation conditions: same as test
- Type of food during acclimation: Sera Vipan (SERA GMBH, 52518 Heinsberg, Germany)
- Feeding frequency during acclimation: Food was provided 3 times per week. Food was given to satisation (4 % of the fish body weight) per feeding day. The test fish were not fed 24 h before test start.
- Health during acclimation: Healthy, no mortality observed
FEEDING DURING TEST: None - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not applicable
- Hardness:
- 67 mg/L
- Test temperature:
- 22.8 - 23.5 °C
- pH:
- 7.46 - 7.91
- Dissolved oxygen:
- 95 - 100 % oxygen saturation
- Salinity:
- Not applicable
- Conductivity:
- 183 μS/cm
- Nominal and measured concentrations:
- Nominal: 100 mg/L (results are based on nominal concentration)
Measured: 88.3 - 105 mg/L (88 to 105 % of nominal) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria of 13 L were used, filled with approximately 10 L of dilution water (dimensions: 25.5/18/28 cm, depth of water: approx. 20 cm) and covered with glass plates
- Type: closed
- Test volume: 10 L per test vessel
- Aeration: No
- Renewal rate of test solution: Every 24 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.047 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Dilution water: Tap water of local origin. The water was filtered on activated charcoal and aerated for at least 24 h to remove chlorine.
- Chlorine: < 0.01 mg/L
- Total organic carbon: < 2 mg/L
- Total Hardness: 67 mg/L
- Acidity: 0.2 mmol/L
- Alkalinity: 0.8 mmol/L
- Conductivity: 183 μS/cm
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: light/dark cycle: 16/8 h
- Light intensity: 741 to 787 Lux
EFFECT PARAMETERS MEASURED: Mortality, all vessels with living fish inspected twice per day
TEST CONCENTRATIONS
- Range finding study: No
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: Concentration based on the derivation of a threshold concentration (TC) from an algae toxicity test (ErC50 (0-72 h) > 100 mg/L) and a daphnia toxicity (EC50 (0-48 h) > 100 mg/L). - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Other abnormalities: None
- Mortality of control: No
- Abnormal responses: No
- Any observations that might cause a difference between measured and nominal values: None
- Effect concentrations exceeding solubility of substance in test medium: No - Sublethal observations / clinical signs:
Table 1: Measured concentration of the test item during the test
Sampling date 0 hours (fresh)
24 hours (aged) 72 hours (fresh) 96 hours (aged) Nominal test item concentration Measured conc. [mg/L] % of nominal Measured conc. [mg/L] % of nominal Measured conc. [mg/L] % of nominal Measured conc. [mg/L] % of nominal 100 mg/L 102 102 88.3 88 105 105 104 104 Control < LOQ < LOQ < LOQ < LOQ LOQ (limit of quantification): 10.0 mg test item/L
Table 2: Cumulative mortality
Nominal test item concentration Cumulative mortality [%] at observation time [hours] Day 0 Day 1 Day 2 Day 3 Day 4 2 h 4 h 23 h 28 h 47.5 h 54 h 70 h 77 h 95 h 96 h 100 mg/L 0 0 0 0 0 0 0 0 0 0 Control 0 0 0 0 0 0 0 0 0 0 The LC50 was estimated to be above the tested concentration in the limit test (> 100 mg test item/L).
Validity:
The study was performed according to the OECD-Guideline No. 203 (2019) for Testing of Chemicals and GLP principles and met the validity criteria:
- The O2 saturation should be > 60 % (this study: ≥ 95 %).
- The mortality in the control group should not exceed 1/7 fish (this study: 0/7 fish).
- Recovery rates of the test item were 80 - 120 % throughout the test.- Validity criteria fulfilled:
- yes
- Conclusions:
- In the 96-hour toxicity test on zebrafish (Danio rerio) according to OECD TG 203, the LC50 was higher than the tested concentration in the limit test (> 100 mg/L).
- Executive summary:
An experimental study was conducted according to OECD 203 and GLP under semi-static conditions as limit test. The limit test was performed with the nominal concentration of 100 mg/L test item. The test concentration was analysed by LC-MS/MS from fresh and aged test solutions. The effect concentrations were based on the nominal concentration as the measured concentrations of the test item were within ± 20 % of the nominal value. All validity criteria of the test guideline were met. Therefore, the study is considered to be fully valid. No effects of the test item were found and after 96 h no mortality was observed. Thus, the LC50 (96 h) was estimated to be > 100 mg/L (nominal).
Reference
Description of key information
In the 96-hour toxicity test on zebrafish (Danio rerio) according to OECD TG 203, the LC50 was higher than the tested concentration in the limit test (> 100 mg/L) (reference 6.1.1-1).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- > 100 mg/L
Additional information
One experimental study was conducted according to OECD 203 and GLP under semi-static conditions as limit test. The limit test was performed with the nominal concentration of 100 mg/L test item. The test concentration was analysed by LC-MS/MS from fresh and aged test solutions. The effect concentrations were based on the nominal concentration as the measured concentrations of the test item were within ± 20 % of the nominal value. All validity criteria of the test guideline were met. Therefore, the study is considered to be fully valid. No effects of the test item were found and after 96 h no mortality was observed. Thus, the LC50 (96 h) was estimated to be > 100 mg/L (nominal).
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