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Diss Factsheets
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EC number: 200-655-4 | CAS number: 67-48-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-08-17 - 1989-08-21
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The experiment was conducted according to the national guideline DIN 38412 L9. No information about GLP compliance is reported. Not all details are described in test report; however, available data indicate that the study meets scientific principles.
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412 L9
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material: No surrogate or analogue material was used.
- Analytical monitoring:
- no
- Details on sampling:
- Samples were taken and evaluated after 72 and 96h. Fluorometric determination of the cell count every 24 h.
- Vehicle:
- no
- Details on test solutions:
- The stock solution contained 100 % of the test substance. No solvents were used, since the water solubility is unlimited for the test substance.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: Chodat
- Source: Göttingen algae collection, SAG 86.81 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No post exposure observation period described.
- Hardness:
- No details available.
- Test temperature:
- 21.5 °C
- pH:
- 0 mg/L: 8.27 (start), 8.32 (end)
10000 mg/L: 8.06 (start), 8.08 (end)
25000 mg/L: 8.08 (start), 7.96 (end)
50000 mg/L: 8.10 (start), 7.79 (end)
100000 mg/L: 8.05 (start), 7.54 (end)
250000 mg/L: 8.03 (start), 7.06 (end)
500000 mg/L: 8.04 (start), 6.59 (end) - Dissolved oxygen:
- No details available.
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- 0, 10, 25, 50, 100, 250 and 500 mg/L
- Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
EFFECT PARAMETERS MEASURED
- Chlorophyll measurement: Fluorometric (impulse fluorometer)
- Spectrophotometric scan: 300 - 780 nm
RANGE FINDING STUDY
- Test concentrations: 5, 50, 500 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 96 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- No further details are reported.
- Results with reference substance (positive control):
- Not reported.
- Reported statistics and error estimates:
- Tallarida, R.J., Jakob, L.S. (1979): The Dose-Response Relation in Pharmacology pp. 98-103, published by Springer.
- Validity criteria fulfilled:
- yes
- Remarks:
- Scientific criteria for acute toxicity testing to aquatic algae seemed to be fulfilled.
- Conclusions:
- The study report describes a valid study, conducted according to a national guideline (DIN 38412 L9). No information about GLP compliance available. Available data confirm that the experiment was well-performed. The test substance was not toxic to aquatic algae (EC50 > 500 mg/L) up to an exposure duration of 96 h.
- Executive summary:
Scenedesmus subspicatus (new name: Desmodesmus subspicatus) was used in an experiment according to the German guideline DIN 38412 L9 in order to test the toxicity of Choline chloride towards freshwater algae (BASF AG, 1989). Test concentrations were chosen as followed: 0 (control), 10, 25, 50, 100, 250 and 500 mg/L.
Potassium dichromate served as reference substance. Samples were taken and evaluated after 72 and 96h. A fluorometric determination of the cell count was performed every 24 h. As results, the EC50(72h) and EC50(96h) resulted in values > 500 mg/L based on growth rate as well as on biomass, respectively. The same applies to the determined EC10 values after 72 h and 96 h.
Reference
Description of key information
No toxicity observed even at highest
concentration level tested.
Scenedesmus subspicatus_DIN 38412 L9:
ErC10(72h) > 500 mg/L,
EbC10(72h) > 500 mg/L,
ErC50(72h) > 500 mg/L,
EbC50(72h) > 500 mg/L,
ErC10(96h) > 500 mg/L,
EbC10(96h) > 500 mg/L,
ErC50(96h) > 500 mg/L,
EbC50(96h) > 500 mg/L
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 500 mg/L
- EC10 or NOEC for freshwater algae:
- 500 mg/L
Additional information
Scenedesmus subspicatus (new name: Desmodesmus subspicatus) was used in an experiment according to the German guideline DIN 38412 L9 in order to test the toxicity of Choline chloride towards freshwater algae (BASF AG, 1989). Test concentrations were chosen as followed: 0 (control), 10, 25, 50, 100, 250 and 500 mg/L.
Potassium dichromate served as reference substance. Samples were taken and evaluated after 72 and 96h. A fluorometric determination of the cell count was performed every 24 h. As results, the EC50(72h) and EC50(96h) resulted in values > 500 mg/L based on growth rate as well as on biomass, respectively. The same applies to the determined EC10 values after 72 h and 96 h.
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