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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21/03/2016 - 07/10/2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 2011
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N-(3-methacrylamidopropyl)-N,N-dimethyldodecan-1-aminium bromide
- Cas Number:
- 129684-50-0
- Molecular formula:
- C21H43N2O.Br
- IUPAC Name:
- N-(3-methacrylamidopropyl)-N,N-dimethyldodecan-1-aminium bromide
- Test material form:
- liquid: viscous
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The study was performed with the unicellular, fresh-water green algae Pseudokirchneriella subcapitata strain, cultured in the laboratories of the Test Facility and originally purchased from the Institute of Plant Physiology of the University of Göttingen, Germany.
The algae were cultured in a climatic chamber at 24 °C ± 2 °C under continuous uniform illumination in the range 4440 - 8880 Lux in the spectral range 400-700 nm. The culture medium was the same of the test medium; the stock cultures were weekly transferred to fresh medium and maintained in continuous shaking to ensure the necessary amount of CO2 and to keep algae in suspension. Cultures containing deformed or abnormal cells were discarded. Only exponentially growing algal cultures have been used to start the test.
The copy of extraction certification is attached in Appendix A.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- Not specified (reconstituted water)
- Test temperature:
- The incubation temperature was continuously monitored during the course of the study by a PT100 probe installed in the environmental test chamber. It was in the range 23.9 – 24.0 °C with a mean value of 24.0 °C and a standard deviation of 0.02°C. The temperature range
provided by OECD guideline is 24 ± 2 C. The temperature values during the test were within the provided range by guideline. - pH:
- At the beginning of the test period pH value was 7.91 for the negative control, for the test item solutions ranged between 7.78– 7.90.
At the end of the test, pH mean values were 9.26 for the negative control and for the test item solutions ranged between 7.84 – 9.67 (mean values among the replicates).
The pH in the negative control varied by a maximum of 1.58 units; slightly higher then the
value recommended by OECD guideline stating that the pH values should not increase more than 1.5 units during the test period. - Dissolved oxygen:
- Not specified (reconstituted water)
- Salinity:
- Not specified (reconstituted water)
- Conductivity:
- Not specified (reconstituted water)
- Details on test conditions:
- The treatment vessels were identified by experiment number, group and replicate number. All the described procedures were conducted under a laminar flow bench to ensure the sterility of the algal cultures.
For the test concentrations and for the negative control, 100 mL capacity conical glass flasks capped with air permeable stoppers were used.
Three replicates for each test concentration, six for negative control were prepared. At test start, 50 mL of test solutions were poured into the test flasks and inoculated with algae to obtain a starting cell density of about 10E4 cells/mL.
The algal inoculum was taken from a pre-culture, started 3 days before the beginning of the test in order to ensure that the assay was performed with exponentially growing algal
population. The cell density of the inoculum was around 10E6 cels/L.
The test flasks were incubated in a climatic chamber, under continuous illumination and
constant shaking. The flasks were randomly distributed under the light and were replaced every day during the test.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.068 mg/L
- 95% CI:
- 0.06 - 0.077
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.022 mg/L
- 95% CI:
- 0.021 - 0.022
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50/Pseudokirchneriella subcapitata at 72 hours for growth rate and yield was 0.068 and 0.022 mg/L, respectively.
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