Iron(III) manganese(II) hexacyanoferrate(II) sodium salts

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Report date:

2020

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Deviations:

no

Test guideline 4

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Version / remarks:

1996

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Iron(III) manganese(II) hexacyanoferrate(II) sodium salts

EC Number:

847-973-5

Cas Number:

2363126-51-4

Molecular formula:

NaxMny1Fey2[Fe(CN)6]z X = 1-2; Y1 = 0.5-0.9; Y2 = 0.1-0.5; Z = 0.67-1.0

IUPAC Name:

Iron(III) manganese(II) hexacyanoferrate(II) sodium salts

impurity 1

Reference substance name:

Water

EC Number:

231-791-2

EC Name:

Water

Cas Number:

7732-18-5

Molecular formula:

H2O

IUPAC Name:

Water

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

See section "Test material information""

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

250 mg/L as CaCO3

pH:

7.8 ±0.2 adjusted (if necessary) with NaOH or HCl

Dissolved oxygen:

Test medium preparation: aerated until the dissolved oxygen concentration was approximately air-saturation value

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 12 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 12 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

12 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Daphnia magna were exposed to the test item in a valid study without showing any toxicity up to the saturation limit and therefore the test item is considered to be not hazardous to daphnia magna.

Executive summary:

Method

Preliminary solubility work conducted indicated that it was not possible to obtain a testable solution of the test item using traditional methods of preparation e.g. ultrasonication and high shear mixing.

A preliminary media preparation trial indicated that a dissolved test item concentration of approximately 12 mg/L was obtained from a slow-stir saturated solution method of preparation indicating this to be the limit of water solubility of this item under test conditions.

Following a preliminary range‑finding test, 20 daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test item at nominal concentrations of 10, 18, 32, 56 and 100% v/v saturated solution for 48 hours at a temperature of approximately 20 °C to 22 °C under semi‑static test conditions. The test item solutions were prepared by stirring an excess (100 mg/L (anhydrous content)) of test item in test water via magnetic stirrer using a stirring rate such that a vortex was formed to give a dimple at the water surface for 3 hours. After the stirring period any undissolved test item was removed by filtration through a 0.2 µm Gelman Acrocap filter (the first approximate 500 mL used to pre‑condition the filter was discarded), to give a 100% v/v saturated solution of the test item. This saturated solution was then further diluted as necessary, to provide the remaining test concentrations. Immobilization and any adverse reactions to exposure were recorded after 24 and 48 hours.

Result

Chemical analysis of the fresh test preparations at 0 and 24 hours showed measured test concentrations to range from 1.1 to 12 mg/L. Analysis of the old test preparations at 24 and 48 hours showed measured concentrations to range from 1.1 to 10 mg/L. There was no significant change in the measured concentrations at 24 and 48 hours and so the results are based on the 0‑Hour measured test concentrations.

Exposure of Daphnia magna to the test item gave EC₅₀values based on the 0‑Hour measured test concentration of greater than 12 mg/L.

The No Observed Effect Concentration (NOEC) was 12 mg/L. This study showed that there were no toxic effects at saturation.