Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 807-276-9 | CAS number: 1421695-64-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Details on test material:
- - Purity: 100%
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Kurume central sewage treatment center (Kurume-shi, Fukuoka, Japan)
- Method of cultivation: Basal culture medium (2 L) was prepared by the method as follows: 10 mL of solution A and each 1 mL of solutions B, C and D prescribed in Guideline 301F were made up to 1 L with purified water (The Japanese Pharmacopoeia, Takasugi Pharmaceutical), and then the pH of this solution was adjusted to 7.4.
- Concentration of sludge in test vessel: 30 mg/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Basal culture medium (2 L): 10 mL of solution A and each 1 mL of solutions B, C and D prescribed in Guideline 301F were made up to 1 L with purified water (The Japanese Pharmacopoeia, Takasugi Pharmaceutical), and then the pH of this solution was adjusted to 7.4.
- Test temperature: 22±1°C
- pH: 7.4
- pH adjusted: yes
- Continuous darkness: yes
- Suspended solids concentration: Decision of additive amount of activated sludge: Additive amount of activated sludge into the test vessel was 3.06 mL on the basis of the concentration of suspended solid in the activated sludge which was determined by in accordance with Japanese Industrial Standards (llS) K 0102-2008 Section 14.1
Result - 2940 mg/L
TEST SYSTEM
- Culturing apparatus: Glass vessel
- Number of culture flasks/concentration: Test solution (sludge + test item) (n=2, Vessel Nos. 1 and 2)
In each test vessel, 30 mg of the test sample was accurately weighed by an electronic analytical balance and added to the mineral medium [the volume subtracting the volume (3.06 mL) of activated sludge from 300 mL], so that the concentration of the test item reached 100 mg/L.
- Measuring equipment: Closed system oxygen consumption measuring apparatus: Temperature controlled bath with measuring unit 0M3100A (OhkuraElectric)
- Test performed in closed vessels due to significant volatility of test substance: yes
- Details of trap for CO2 and volatile organics if used: Absorbent for carbon dioxide: Soda lime No. 1 (for absorption of carbon dioxide, Wako Pure chemical Industries)
- Other: Incubation duration 28 days (under dark conditions); Volume of test solution - 300 mL
CONTROL AND BLANK SYSTEM
- Test solution (sludge + sodium benzoate) (n=l, Vessel No.5)
In one test vessel, 30 mg of sodium benzoate was accurately weighed by an electronic analytical balance and added to the mineral medium [the volume subtracting the volume (3.06 mL) of activated sludge from 300 mL], so that the concentration of sodium benzoate reached 100 mg/L.
- Test solution (control blank) (n=2, Vessel Nos. 3 and 4)
In each test vessel, nothing was added to the mineral medium [the volume subtracting the volume (3.06 mL) of activated sludge from 300 mL].
Observation of test solution: During the incubation period, the appearance of the test solution was observed once a day.
Measurement of biochemical oxygen demand (BOD): During the incubation period, BOD of the test solutions was measured continuously by a closed system oxygen consumption measuring apparatus. The incubation temperature was measured and recorded once a day.
Measurement of pH: After the end of the incubation, the pH of the test solutions (sludge + test item) and the test solutions (control blank) were measured.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- or acetic acid, sodium salt
Results and discussion
- Test performance:
- This test was judged to be valid because all the values in this test met the criteria. No adverse effects on the reliability of this test were noted.
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 88
- Sampling time:
- 28 d
- Remarks on result:
- other: The percentage biodegradation by the BOD were 85% and 90%. Mean = 88%
- Details on results:
- The results indicate that almost all the test item underwent complete degradation under the test conditions of this study. Substances are considered to be readily biodegradable if percentage biodegradation by BOD is equal to or greater than 60% of the theoretical value within ten days of the level achieving 10%. The percentage biodegradations after the l0-day window were 62% and 70% in this study. Therefore, the test item is a readily biodegradable substance.
BOD5 / COD results
- Results with reference substance:
- Percentage biodegradation of sodium benzoate by BOD after 14 days was 92%.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The results of this test indicate that the test item is a readily biodegradable substance.
- Executive summary:
A 28-day biodegradation study was conducted according to OECD Guidelines for Testing of Chemicals, No 301F, July 17, 1992, "Ready Biodegradability: MANOMETRIC RESPIROMETRY TEST". Test, control and reference vessels were incubated at 22±1°C in the dark. The oxygen content was measured continuously by a closed system oxygen consumption measuring apparatus. The percentage biodegradation was calculated and rounded off to the whole number. Blank and reference substance tests met validation criteria and the test was judged acceptable.
The percentage biodegradations by the BOD were 85% and 90% for the two test vessels. These results indicate that almost all the test item underwent complete degradation under the test conditions of this study. Substances are considered to be readily biodegradable if percentage biodegradation by BOD is equal to or greater than 60% of the theoretical value within ten days of the level achieving 10%. The percentage biodegradations after the l0-day window were 62% and 70% in this study. Therefore, the test item is a readily biodegradable substance.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)
Although ECHA is providing a lot of online material in your language, part of this page is only in English. More about ECHA’s multilingual practice.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
the-echa-website-uses-cookies
find-out-more-on how-we-use-cookies