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EC number: 700-903-6 | CAS number: 255830-15-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- The study was conducted according to an appropriate national standard method but full details are not available. It was not conducted under GLP and no analytical monitoring was carried out.
- Qualifier:
- according to guideline
- Guideline:
- other: In house protocol: MIC Environmental Assessment Method for Conducting Acute Toxicity Tests with Daphnia magna (see note below)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: US EPA (1975) Methods for acute toxicity tests with fish, macroinvertebrates and amphibians. EPA 660/3-75-009.
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not applicable
- Principles of method if other than guideline:
- The In house protocol references Grueber and Adams (1980)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: For each test concentration the appropriate amount of the test compound was pipetted into 1L of dilution water and shaken vigorously for 1 min. The solution was then divided into 2 200 ml aliquots in triplicate beakers to provide the appropriate replication. The remaining 400 ml were used for 0 h DO, pH, alkalinity and hardness determinations.
- Controls: Just dilution water. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: The daphnids used in the test were obtained from a laboratory culture at the MIC laboratory.
- Age at study initiation (mean and range, SD): <24 h
- Method of breeding: according to lab SOP
- Feeding during test: no
ACCLIMATION
- Acclimation period: not reported
- Acclimation conditions (same as test or not): not reported
- Type and amount of food: Daphnia were fed a mixture of Purina trout chow and alfalfa daily.
- Health during acclimation (any mortality observed): not reported - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- The alkalinity ranged from 236 to 304 mg/L and 250 to 300 mg/L in the low and high test concentration vessels respectively.
- Test temperature:
- Average temperature of 21.2 degrees C.
- pH:
- The pH in the control medium ranged between 7.2-8.4. The pH in the test media ranged between 2.0 and 8.2.
- Dissolved oxygen:
- The dissolved concentrations for the treatments were not reported due to measurement interference from the test materials. However, DO on the control water ranged from 6.2-9.4 mg/L. DO was measured by the Winkler titration method.
- Nominal and measured concentrations:
- Nominal test concentrations were 125, 250, 500, 1000 and 2000 mg/l as active acid
- Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Material, size, headspace, fill volume: 250 ml beakers containing 200 ml of test medium.
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water from St. Peters, Missouri.
- Alkalinity: 303 mg/L CaCO3, hardness 297 mg/L CaCO3
- Culture medium different from test medium: not reported
- Intervals of water quality measurement: DO, pH, alkalinity, hardness and temperature of test solutions were monitored at the initiation (control only) and termination of the test in the high, middle and low concentrations.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: not reported
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility at 0, 24 and 48 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: not reported
- Results used to determine the conditions for the definitive study: EC50 833 mg/L - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 125 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: immobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 297 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: immobility
- Remarks on result:
- other: 95% CL 262-342
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 545 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: immobility
- Details on results:
- - Mortality of control: 0%
- Reported statistics and error estimates:
- EC50 values and 95% confidence intervals calculated using an LC50 computer programme developed by Stephen et al (1978).
- Validity criteria fulfilled:
- no
- Remarks:
- pH very low at the highest concentrations tested. However the reported EC50 value is high enough to determine toxicity that the test substance is of low intrinsic toxicity.
- Conclusions:
- A 48 h EC50 value of 297 mg active acid/L has been determined for the effects of ATMP acid on the immobility of the freshwater flea D. magna.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995-10-18 to 1995-10-20
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Test procedure in accordance with national standard methods (based on ISO guideline) with acceptable restrictions. No data on GLP or analytical monitoring available. The test was conducted in a reputable and established laboratory according to an appropriate guideline, on the basis of this we can assign a reliability of 2 since no deviations from the guideline are stated even though the data presented is from a summary report only.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: draft method ISO/CD 14669
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Brixham Environmental Laboratory SOP BA207:03.
- Deviations:
- no
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- other: Acartia tonsa
- Details on test organisms:
- TEST ORGANISM
- Common name: Acartia
- Age at study initiation (mean and range, SD): 24.5 (+/-3) days - Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
- Hardness:
- Not reported, assumed to meet guideline criteria.
- Test temperature:
- 20 deg C (+/- 1)
- pH:
- Not reported, assumed to meet guideline criteria.
- Dissolved oxygen:
- Not reported, assumed to meet guideline criteria.
- Salinity:
- Not reported, assumed to meet guideline criteria.
- Nominal and measured concentrations:
- Nominal test concentrations were 56, 100, 180, 320, 560 and 1000 mg/l (as test substance).
- Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 20 - Reference substance (positive control):
- yes
- Remarks:
- 3,5, dichlorophenol
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 94 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: (95% CL 74.5-115)
- Details on results:
- - Mortality of control: 5%
- Reported statistics and error estimates:
- Not reported, assumed to meet guideline criteria.
- Validity criteria fulfilled:
- not specified
- Remarks:
- not enough data to validate, assumed to meet guideline criteria.
- Conclusions:
- A 48 h LC50 value of 94 mg/L (as active acid) has been determined for the effects of the test substance on mortality of the marine invertebrate Acartia tonsa.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Control data were not reported and pH was lower than the recommended range stated in the guideline. It was not possible to exclude that the effects seen are related to pH effects.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- yes
- Remarks:
- low pH, controls data not reported, no analytical monitoring
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: the test substance was dissolved directly into the water. Unspecified whether a stock solution or vessels.
- Controls: only dilution water contro (negative control) + dilution water acidified waters to the pH of the highest control (positive control) - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: bred in the laboratory
- Age at study initiation (mean and range, SD): <24 hours
- Method of breeding: the test animals were bred in glass containers. To avoid the necessity of adaptation prior to test, the water used in the test was used for breeding.
- Feeding during test: no
ACCLIMATION
- Acclimation period: none
- Acclimation conditions (same as test or not): same water used in breeding and in test. Photoperiod differed (12:16 h, light:dark during breeding, while the study was conducted in darkness).
- Type of food: during breeding animals were fed with green algae (S. capricornutum) laboratory grown
- Feeding frequency: 3 times per week
- Health during acclimation (any mortality observed): not reported - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20⁰C
- pH:
- range: 7.7 to 7.8 in negative control, 4.4 to 4.5 in positive control, 4.4 to 5 in exposure media
- Dissolved oxygen:
- range:9.4 to 10.2 mg/l in negative control, 6 to 9.5 mg/l in positive control, 8 8.1 to 9.8 mg/l in exposure media
- Nominal and measured concentrations:
- Nominal concentrations test substance: 0, 678, 821, 994, 1203 and 1445 mg/l.
Nominal concentrations as active salt: 0, 290, 355, 430, 515 and 620 mg/l.
Nominal concentrations as active acid: 0, 185, 225, 270, 325 and 390 mg/l. - Details on test conditions:
- TEST SYSTEM
- Test vessel: not reported
- Material, fill volume: glass vessels with 10 ml water
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate: 1 animal/2ml
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Culture medium different from test medium: no
- Intervals of water quality measurement: DO, temperature and pH were measured at 0 and 48 hours.
OTHER TEST CONDITIONS
- Adjustment of pH: HCl 1N
- Photoperiod: none, the study was conducted in darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation was measured at 24h and 48 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.21
- Range finding study: the concentrations for the study were based on preliminary findings, however details are not reported. - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 970 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: (887-1063)
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 420 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- salt
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 260 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- acid
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 485 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: not reported
- Reported statistics and error estimates:
- Bliss-Finney probit analysis. However 100% mortality was not recorded
- Validity criteria fulfilled:
- no
- Remarks:
- the pH of the test medium was below 5
- Conclusions:
- A 48 h EC50 value of 970 mg/l as test substance, 420 mg /l as active salt and 260 mg/l as active acid has been determined for the effects of the test substance to the freshwater invertebrate D. magna. It cannot be excluded that the effects seen have been influenced by the pH of the test system (4-5 across the exposure concentrations). However the study does support the low short term toxicity of the substance.
Referenceopen allclose all
Table 1. Summary of findings (average).
Nominal concentrations (mg/L) | Percent immobilisation | |
24 h | 48 h | |
Control | 0 | 0 |
125 | 0 | 6.7 |
250 | 0 | 13.3 |
500 | 40 | 60 |
1000 | 100 | 100 |
2000 | 100 | 100 |
Result expressed as nominal concentration. Properties of the test substance and evidence from other studies (where concentrations were
measured) indicate that nominal and
measured concentrations are likely to be in good agreement.
The pH data suggest that the acidity of the higher concentration test solutions is likely to have contributed to the effects observed in the test.
Result expressed as nominal concentration. Properties of the test substance and evidence from other studies (where concentrations were
measured) indicate that nominal and measured concentrations are likely to be in good agreement. Percentage mortality at each test concentration at the end of the test (48 hours):
Table 1. Results
Nominal conc. (mg/L) | % mortality |
|
24 h | 48 h | |
3,5 Dichlorophenol (1 mg/L) | 40 | 100 |
Control | 0 | 5 |
56 | 0 | 5 |
100 | 0 | 20 |
180 | 0 | 10 |
320 | 100 | 100 |
560 | 100 | 100 |
1000 | 100 | 100 |
24 LC50 (95% confidence intervals): 240 (180-320) mg/L as test substance. Equivalent to 120 mg active acid/L.
Table 1. Summary of immobility recorded.
Nominal concentration (mg/l) | Immobile # | Total % | |
24 h | 48 h | ||
678 | 0 | 3 | 15 |
821 | 1 | 5 | 30 |
994 | 2 | 8 | 50 |
1203 | 6 | 9 | 75 |
1456 | 9 | 9 | 90 |
Description of key information
Key (m) 48h LC50 94 mg/L, Acartia tonsa, read-across from ATMP-H, (reliability 2, Zeneca 1995a).
WoE (fw) 48 h EC50 260 mg active acid/L, Daphnia magna (reliability 4, RBM 1991);
WoE (fw) 48h EC50 297 mg active acid/L, Daphnia magna, read-across from ATMP-H, (reliability 2, Monsanto 1981);
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 260 mg/L
Marine water invertebrates
Marine water invertebrates
- Effect concentration:
- 94 mg/L
Additional information
Data are available with ATMP-N-oxide-5K (CAS 255830-15-0) and the structurally analogous substance ATMP-H (CAS 6419-19 -8).
A 48 h EC50 value of 260 mg/l as active acid, has been determined for the effects of the test substance to the freshwater invertebrate Daphnia magna.
No control results have been reported. Therefore it cannot be excluded that the effects seen may have been influenced by the pH of the test system (4-5 across the exposure concentrations) and the study has been assigned a reliability Klimisch score of 4. The study does however support the low short term toxicity of the substance. The results also agree with the data read-across from the structurally analogous substance ATMP-H (CAS 6419-19 -8). The study does therefore add further weight of evidence to the low short-term toxicity of the substance, albeit observational.
Data with ATMP-H has been brought to add for weight of evidence to support the category’s low short-term toxicity to freshwater invertebrates. A short-term toxicity to marine invertebrates is also available and has been assigned as key as it is the only study available with marine organisms. A 48 h EC50 value of 297 mg active acid/L has been determined for the effects of ATMP-H (CAS 6419-19-8) on the immobility of the freshwater flea Daphnia magna. A 48 h LC50 value of 94 mg active acid/L has been determined for the effects of ATMP-H (CAS 6419-19-8) on the immobility of the marine invertebrate Acartia tonsa.
The read-across justification is attached in IUCLID Section 13.
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