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EC number: 931-472-4 | CAS number: 182700-89-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
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- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
An activated sludge respiration inhibition test reported a 3-hour EC50 of >100 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
In a GLP study conducted according to OECD Guideline 209 and EU Method C.11, the test material was studied for its potential inhibitory effect on the respiration of activated sludge microflora from a predominantly domestic waste sewage source.
Since the test material was insoluble, it was prepared in an aqueous solution of tetrapropylenebenzene sulphonate, sodium salt (TPBSNa) and ultrasonicated. Calcium chloride was added to stabilise the dispersion, further sonicated and diluted with distilled water, sonicated then stirred vigorously for 30 minutes. The test material was added to Drechsel bottles containing 300 mL distilled water to give final concentrations of 1, 3.4, 10, 20, 50 or 100 mg/L after addition of the activated sludge inoculum. At 15-minute intervals, 200 mL of activated sludge was added to each bottle and the contents aerated with moist air. The final concentration of flocculated activated sewage sludge suspended solids was 0.8 g/L. Duplicate bottles were prepared for the controls: synthetic sewage, inoculum and water or synthetic sewage, inoculum, water and TPBSNa. After 3 h exposure oxygen consumption was measured using a dissolved oxygen electrode and meter connected to a chart recorder, the respiration rate determined from the linear portion of the graph divided by the concentration of the flocculated activated sewage sludge suspended solids and the percentage inhibition of total oxygen consumption was calculated.
The test material did not inhibit the specific respiration rate at any of the doses tested, which was probably due to its insolubility and lack of bioavailability. No analysis was carried out on of the actual concentrations in the aqueous medium which were likely to be considerably less than the nominal concentrations. The EC50 based on the nominal doses (which includes dissolved, undissolved and adsorbed concentrations of the test material was greater than 100 mg/L (no upper limit was investigated). An EC50 of 20 mg/L (95 % CL 14 to 28 mg/L) was obtained for the positive control by interpolation of the dose-response curve using non-linear regression software. The respiration rates of control and vehicle control were within 15 % of their respective means, demonstrating the validity of the assay.
In conclusion, an EC50 of >100 mg/L was calculated for the test material in an activated sludge respiration inhibition test. However, since the test material was insoluble, the actual concentration bioavailable (if any) was likely to be considerably lower than the nominal concentrations.
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