Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 916-918-8 | CAS number: -
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 May 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Stock solution prepared with test substance directly weighed into test medium (1000 mg/L nominal).
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Laboratory wastewater treatment plant (Leverkusen, Germany)
Sampling date: 16 May 1991
Concentration: 6 g dry substance / L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Nominal and measured concentrations:
- 10, 18, 32, 56, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: bottles
- Aeration: no
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Test medium according to ISO 8192-1986
OTHER TEST CONDITIONS
- Adjustment of pH: no - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 6.06 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 18.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: 70.3% respiration inhibition at 20 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The 3-h EC10 and EC50 to activated sludge respiration inhibition were determined at 6.06 and 18.1 mg/L, respectively.
Reference
Description of key information
The 3-h EC10 and EC50 to activated sludge respiration inhibition were determined at 6.06 and 18.1 mg/L, respectively.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 18.1 mg/L
- EC10 or NOEC for microorganisms:
- 6.06 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
