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EC number: 606-059-4 | CAS number: 185756-31-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 Apr 2004 to 22 Apr 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1993
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 1987
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Version / remarks:
- 1986 (Method B)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Appearance: White, powdered solid
- Water-solubility: 29 mg/L
- Homogeneity: homogeneous
- Storage conditions: at room temperature - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was added in the required amount to the test vessel with about 180 mL deionized water. The mixture was stirred for 22 ± 2 hours to obtain an optimal solution. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge from laboratory wastewater plant treating municipal sewage.
- Preparation of inoculum for exposure: The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated during the night. 50 mL were added to a total volume of 250 mL to obtain the desired dry substance concentration. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.0 - 7.7
- Dissolved oxygen:
- 6.7 - 8.8
- Nominal and measured concentrations:
- - Nominal concentrations: 0 (control), 100, 500 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 mL)
- Test volume: 250 mL
- Replicates: 3 for the control, 1 for each test concentration, 1 for each reference substance concentration
- Synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium
- Duration of measurement during oxygen consumption: 8 - 10 minutes
- Sludge concentration in the test vessel: 1 g/L Dw
OTHER TEST CONDITIONS
- Adjustment of pH: pH was adjusted after addition of synthetic medium, before the inoculum was added and the test started
EFFECT PARAMETERS MEASURED:
- The oxygen concentration (mg O2/L) was measured at the test start and after 3 hours. The duration of the measurement of oxygen consumption was 8 - 10 minutes. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 750 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Inhibition of the total respiration was observed at all test concentrations, starting from 12% inhibition at 100 mg/L and increasing to 12% at 500 mg/L and 23% inhibition at 1000 mg/L, respectively. Effect concentrations exceeding solubility of substance in test medium: EC20, EC50 and EC80 were all above the water solubility of the test substance (29 mg/L).
- Results with reference substance (positive control):
- The 3-h EC50 of the reference substance was determined to be about 7 mg/L.
- Reported statistics and error estimates:
- The nominal concentrations which indicate a respiration inhibition of 20, 50 and 80 % were taken from the graph of the inhibition curve.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The inhibition of the degradation activity of activated sludge is not anticipated when introduced to biological treatment plants in appropriate low concentrations.
Reference
Table: measured data on oxygen consumption in the test vessels
Vessel no.: |
BC1 |
BC2 |
BC3 |
mv BC |
TS1 |
TS2 |
TS3 |
RS1 |
RS2 |
RS3 |
Concentration of test substance (mg/L) |
- |
- |
- |
- |
1000 |
500 |
100 |
1 |
10 |
100 |
Added volume of stock solution (mL/vessel) |
- |
- |
- |
- |
- |
- |
- |
0.5 |
5.0 |
50.0 |
Direct addition (mg/vessel) |
- |
- |
- |
- |
250.0 |
125.0 |
25.0 |
- |
- |
- |
Synthetic medium (mL/vessel) |
8 |
8 |
8 |
- |
8 |
8 |
8 |
8 |
8 |
8 |
Inoculum (mL/vessel) |
50 |
50 |
50 |
- |
50 |
50 |
50 |
50 |
50 |
50 |
Filled up to the total volume with deionized water (mL/vessel) |
250 |
250 |
250 |
- |
250 |
250 |
250 |
250 |
250 |
250 |
Oxygen concentration start (mg O2/L) |
7.3 |
7.3 |
6.7 |
- |
6.7 |
7.0 |
7.1 |
6.9 |
8.6 |
8.8 |
Oxygen concentration after 6 min (mg O2/L) |
4.6 |
4.6 |
4.2 |
- |
4.7 |
4.8 |
4.8 |
4.5 |
7.5 |
8.5 |
Oxygen consumption rate (mg O2/L*6 min) |
2.7 |
2.7 |
2.5 |
2.6 |
2.0 |
2.2 |
2.3 |
2.4 |
1.1 |
0.3 |
Oxygen consumption rate (mg O2/L*h) |
27 |
27 |
25 |
26 |
20 |
22 |
23 |
24 |
11 |
3 |
Specific oxygen consumption rate (mg O2/g*h) |
27 |
27 |
25 |
26 |
20 |
22 |
23 |
24 |
11 |
3 |
Change in oxygen consumption rate compared with the blank control (%) |
- |
- |
- |
- |
23 |
15 |
12 |
8 |
58 |
88 |
BC = blank control, mv = mean value, TS = test substance, RS = reference substance
ADDITIONAL EFFECT VALUES
- 3-h EC20: ca. 750 mg/L (nominal)
- 3-h EC80: > 1000 mg/L (nominal)
Description of key information
The inhibition of the degradation activity of activated sludge is not anticipated when introduced to biological treatment plants in appropriate low concentrations.
Key value for chemical safety assessment
Additional information
The toxicity of the substance to microorganisms was investigated in an activated sludge respiration inhibition study according to OECD TG 209 and in compliance with GLP criteria. In this study non-adapted activated sludge from a predominantly domestic sewage was exposed to nominal test substance concentrations of 0 (control), 100, 500 and 1000 mg/L for 3 hours. Test concentrations were not analytically verified. The effects on microorganisms was determined by measuring total respiration rates and comparing these to an unexposed control. Inhibition of respiration rates increased concentration-dependently reaching 23% at the highest concentration tested. Based on these findings, the 3-h EC50 and EC20 values were determined at >1000 mg/L and ca. 750 mg/L, respectively.
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