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EC number: 610-555-6 | CAS number: 50650-75-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996-05-09 to 1996-06-11
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed acc. to Guideline, is well documented and run under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-methyl-6-(2,4,4-trimethylpentyl)-2H-pyran-2-one
- EC Number:
- 610-555-6
- Cas Number:
- 50650-75-4
- Molecular formula:
- C14H22O2
- IUPAC Name:
- 4-methyl-6-(2,4,4-trimethylpentyl)-2H-pyran-2-one
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
Concentration of stock solution: 20000 mg/L
Preparation of stock solution:
20000mg of test substance was weighed in together with 800mL water.
Solution was stirred for 5 min. twice at 19°C
Afterwards it was treated 15min. twice by ultrasound
The pH was adjusted from 4.3 to 7.5 by using 10mol/L NaOH-solution.
The solution was then treated with ultrasound (15min) and was stirred (5min).
The test substance was only partially solublized. It was then filtrated and brought up to 1L.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge was used.
source: municipal waste treatment plant Frankfurt/Sindlingen
Treatment: According to the guideline. The bacterial culture was adapted neither to the test substance nor to the reference substance before the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Remarks on exposure duration:
- The exposure period was reduced to 30 Min because of the volatility of the test substance. Results are related to the analytical measurements.
Test conditions
- Test temperature:
- 19.7 - 20.3°C
- pH:
- 7.3 - 7.4
- Nominal and measured concentrations:
- Test was run in duplicates. Measured test concentrations were 5.0, 10.2, 26.5, 37.2, 47., 84.7, 218.6 and 593.0
- Details on test conditions:
- Because of the high volatility of the test substance in aqueous solution, the incubation period was shortened to 30 minutes. Initially and after each test at the end the test substance content was determined via the dissolved organic carbon (DOC). Based on the analytical results the stability of the test concentrations cannot be guaranteed over the test time. At some concentration levels, a significant decrease (> 20%) of the test substance during the test period was found. Therefore, all concentration data refer to the values which were analytically determined directly after recording the activity curves.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorphenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- 36 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks on result:
- other: confidence interval 15 - 67 mg/L
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 337 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks on result:
- other: confidence interval 162 - 1597 mg/L
- Duration:
- 30 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 555 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The calculated EC50 is 360 mg/L wiith a confidence intervall of 173-1706 mg/L.
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