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EC number: 609-904-5 | CAS number: 41263-74-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011-10-04 to 2011-10-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 4-(methylamino)-3-nitrobenzoic acid
- EC Number:
- 609-904-5
- Cas Number:
- 41263-74-5
- Molecular formula:
- C8 H8 N2 O4
- IUPAC Name:
- 4-(methylamino)-3-nitrobenzoic acid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Prior to the test starting, 0.5 litre of a stock solution of 100 mg/L as test item was prepared by direct weighting into reconstituted water (0.0502 g of test item into 502 mL of reconstituted water). The obtained solution appeared orange and it showed orange insoluble grains, therefore it was sonicated for 15 minutes and then it was mixed by the mean of a magnetic stirrer for about 15 minutes in dark conditions to achieve the total solubilisation of test item.
After stirring, it was a clear and homogeneous solution.
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 18.8 - 20.5°C
- pH:
- 7.12 - 7.88
- Dissolved oxygen:
- 6.48 - 6.81
- Details on test conditions:
- -20 animals per vessel (test item and negative control (reconstituted water))
-Test concentration based on range-finding study - Reference substance (positive control):
- yes
- Remarks:
- Daphnia clone sensitivity check with potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- IC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- other: IC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- 24 h IC50 = 1.02 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- IC50 at 24 and 48 hours >100 mg/L
- Executive summary:
The acute toxicity of the test item BIBR 1048 Aminoacido to Daphnia magna was determined in a 48-hour limit static test according to the OECD Guideline for Testing of Chemicals, No. 202 (2004) and to Council Regulation EC 440/2008 (C.2).
For this purpose, juvenile daphnids (< 24 hours old at initiation of the test) were exposed to an aqueous test medium containing the test item at the only concentration of 100.0 mg/L, corresponding to 99.1 mg/L, corrected for its purity, 99.1% w/w.
20 organisms for the test item solution and 20 organisms for negative control were exposed.
The exposed organisms were checked for immobilization 24 and 48 hours after test initiation.
The pH of the test media after preparation was 7.12 for test item solution and 7.40 for negative control. The incubation temperature was in the range 18.0 – 20.5 °C, thus within to the OECD recommended range (20 +- 2°C).
Light intensity during the 48 hours of test period was in the range 904 – 950 Lux, thus within the provided range 200-1500 Lux.
At the end of test period, in the negative control 0% of immobilization was observed and no daphnid was found to be trapped on the test water surface. These values comply with the validity criterion of the test, that provide a maximum immobilization and/or a maximum number of daphnids trapped into water surface of 10% in the negative control medium at the end of the test.
The obtained experimental results allowed to assess the IC0, the IC50 and the IC100 values at 24 and 48 h. The results, based on the nominal concentrations of the test item, were as reported in the result table above.
The IC50 values were determined from the Raw Data by the statistical analysis in comparison with the negative control (linear interpolation analysis) while IC0 and IC100 values at each observation time were directly extracted from the Raw Data.
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