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EC number: 500-241-6 | CAS number: 69011-36-5 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- Please refer to the Analogue Approach Justification provided in Section 13.
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Denton Wastewater Treatment Plant, Denton, Maryland, USA
- Pretreatment: The sludge was sieved using a 2-mm screen and then aerated for approximately four hours. After the aeration period, the sludge was homogenized in a blender at medium speed for approximately two minutes and then allowed to settle for approximately 30 minutes.
- Concentration of sludge:
- Initial cell/biomass concentration: - Duration of test (contact time):
- 28 d
- Initial conc.:
- 10 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 2470 mL of NANO®pure water, 3 mL calcium chloride solution (2.75%), 3mL of ferric chloride solution (0.025%), 3 mL of magnesium sulfate solution (2.25%), 30 mL of phosphate buffer (pH 7.4), 30 mL of the activated sludge inoculum
- Test temperature: 18.6 to 21.8°C
- pH: 7.2
- pH adjusted: no
TEST SYSTEM
- Culturing apparatus: amber 4-liter bottles
- Number of culture flasks/concentration: 2
- Details of trap for CO2 and volatile organics if used: 100 mL of 0.5 N KOH
SAMPLING
- Sampling frequency: CO2 traps were removed for analysis on days 1, 6, 8, 13, 15, 19, 22 and 26.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 replicates
- Reference control: 2 replicates
- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 50.7
- Sampling time:
- 28 d
- Remarks on result:
- other: mean of two replicates
- Results with reference substance:
- Biodegradation rate: 92.5 % after 28 days, 78.3% after 15 days
- Validity criteria fulfilled:
- yes
- Remarks:
- please refer to "Any other information on results incl. tables"
- Interpretation of results:
- not readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Study period:
- 12 Jan - 10 Feb 2006
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- Please refer to the Analogue Approach Justification provided in Section 13.
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- no
- Remarks:
- The test facility is certified according to GLP, the performance of the study occurred under GLP-near conditions without the formal procedure of GLP quality assurance.
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from the municipal wastewater treatment plant Breisgauer Bucht, Germany treating predominantly domestic wastewater
- Pretreatment: The activated sludge was washed twice by settling the sludge, decanting the supernatant and resuspending the sludge in aerated tap water
- Concentration of sludge: 30 mg solids/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 - 20.1 mg/L
- Based on:
- other: TOC
- Initial conc.:
- 43.5 - 43.6 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: acc. to guideline
- Test temperature: 21.0 - 21.5 QC
- Suspended solids concentration: 30 mg/L dry solids
- Continuous darkness: diffuse light
TEST SYSTEM
- Culturing apparatus: Gas wash bottles (2000 ml volume) with lateral connecting pieces for butyl rubber septums were used as reactors. The CO2 produced in the reactors was absorbed in two 250 ml gas wash bottles in series.
- Number of culture flasks/concentration: 3
- Details of trap for CO2 and volatile organics if used: 0.2 M NaOH
SAMPLING
- Sampling frequency: on days 0, 4, 7, 11, 14, 21, and 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: 3 replicates
- Toxicity control: 1 replicate - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 60.2
- Sampling time:
- 28 d
- Remarks on result:
- other: mean of three replicates
- Details on results:
- The degradation in the toxicity control was 68.6% within 14 days and 76.1% within 28 days
- Results with reference substance:
- Biodegradation rate after 14 d: 97,43 (mean of three replicates)
- Validity criteria fulfilled:
- yes
- Remarks:
- please refer to "Any other information results incl. tables"
- Interpretation of results:
- readily biodegradable, but failing 10-day window
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 03 Mar - 31 Mar 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- Please refer to the Analogue Approach Justification provided in Section 13.
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from the aeration tank of a municipal waste water treatment plant (ARA Werdhölzli, Zürich), not adapted, not pre-conditioned
- Concentration of sludge: 30 mg/I of suspended solids - Duration of test (contact time):
- 28 d
- Initial conc.:
- 18.5 - 18.7 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Aerobic mineral salts medium prepared with deionised water
- Test temperature: 22±0.5 DC
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes, temperature-controlled dark room
TEST SYSTEM
- Culturing apparatus: 2500 ml closed glass bottle containing a total volume of test solution of 2000 ml;
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: aerated with CO2-free air
- Measuring equipment: gas-absorption bottles containing 125 ml of 0.15 M NaOH
- Details of trap for CO2 and volatile organics if used: NaOH
SAMPLING
- Sampling frequency: daily
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 replicates
- Procedure control: 1 replicate
- Abiotic sterile control: no
- Toxicity control: no - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 75
- Sampling time:
- 28 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 82
- Sampling time:
- 28 d
- Results with reference substance:
- The reference substance reached 97% after 14 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- Please refer to "Any other information on results incl tables"
- Interpretation of results:
- readily biodegradable
Referenceopen allclose all
Table 1: Ultimate biodegradation after x days [% of ThCO2]
|
Cumulative Percent of Theoretical Carbon Dioxide Evolved1 |
|||
Day |
Reference 1 |
Reference 2 |
Test substance |
Test substance |
1 |
0.0 |
-0.2 |
-0.1 |
0.4 |
6 |
56.4 |
52.5 |
10.0 |
9.9 |
8 |
64.6 |
62.9 |
13.6 |
13.2 |
13 |
71.0 |
78.1 |
25.8 |
24.0 |
15 |
75.3 |
81.3 |
30.6 |
29.7 |
19 |
79.3 |
39.1 |
38.3 |
37.8 |
22 |
84.7 |
88.6 |
41.8 |
41.1 |
26 |
87.4 |
89.8 |
45.3 |
46.0 |
29 |
91.3 |
93.6 |
51.6 |
49.7 |
Mean |
92.5 |
50.7 |
Table 2: Validity criteria for OECD 30 B1.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
< 5% |
yes |
Percentage degradation of the reference compound reached the pass level by day 14 (≥ 60%). |
78.3% after 15 days |
yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
n.a. |
n.a. |
The IC content of the test substance suspension in the mineral medium at the beginning of the test must be less than 5% of the TC. |
0 |
yes |
The total CO2 evolution in the inoculum blank at the end of the test should not normally exceed 40 mg/L medium. |
5.2 mg/L |
|
Table 1: Ultimate biodegradation after x days [% of ThCO2]
Flask |
Day |
|||||||
|
0 |
4 |
7 |
11 |
14 |
21 |
28 |
28 (after acidification) |
Test 1 |
0 |
18.3 |
33.5 |
46.0 |
51.0 |
61.6 |
67.7 |
66.5 |
Test 2 |
0 |
17.0 |
29.8 |
40.5 |
51.3 |
56.4 |
59.7 |
55.5 |
Test 3 |
0 |
17.2 |
32.5 |
42.0 |
49.6 |
53.9 |
57.8 |
58.7 |
Reference 1 |
0 |
79.4 |
90.6 |
93.4 |
93.3 |
100.6 |
98.3 |
97.3 |
Reference 2 |
0 |
79.1 |
92.8 |
100.1 |
100.9 |
108.8 |
105.6 |
106.1 |
Reference 3 |
0 |
83.1 |
98.1 |
96.1 |
98.1 |
108.9 |
105.4 |
107.9 |
Toxicity control |
0 |
41.2 |
55.3 |
65.6 |
68.6 |
74.0 |
74.7 |
76.1 |
Table 2: Validity criteria for OECD 301.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
<10% |
yes |
Percentage degradation of the reference compound reached the pass level by day 14 (≥ 60%). |
>90% |
yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
68.6% |
yes |
The IC content of the test substance suspension in the mineral medium at the beginning of the test must be less than 5% of the TC. |
0 |
yes |
The total CO2 evolution in the inoculum blank at the end of the test should not normally exceed 40 mg/L medium. |
29.5 mg/L |
yes |
Table 1: Validity criteria for OECD 301 B
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
<5% |
yes |
Percentage degradation of the reference compound reached the pass level by day 14 (≥ 60%). |
97% |
yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
n.a. |
n.a. |
The IC content of the test substance suspension in the mineral medium at the beginning of the test must be less than 5% of the TC. |
0 |
yes |
The total CO2 evolution in the inoculum blank at the end of the test should not normally exceed 40 mg/L medium. |
18.5 mg/L |
yes |
|
|
|
Table 2 CO2 produced by the test units, the inoculum blank and the procedure control
|
Inoculum blank |
Procedure control blank * (sodium benzoate) |
Test unit 1 |
Test unit 2 |
|
|||
Time (days |
Total CO, release in test sample [mgIC/I] |
Total CO, release in test sample [mgIC/I] |
Degradation [%] |
Total CO, release in test sample [mgIC/I] |
Degradation [%] |
Total CO, release in test sample [mgIC/I] |
Degradation [%] |
Mean Degradation [%] Test unit 1+2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0.3 |
6.5 |
33.2 |
-0.3 |
-3.2 |
0 |
-1.6 |
-2.4 |
2 |
1.9 |
12.3 |
55.6 |
1.9 |
0.0 |
2.2 |
1.6 |
0.8 |
5 |
6.3 |
21.2 |
79.7 |
9.5 |
17.1 |
10.8 |
24.3 |
20.7 |
8 |
8.3 |
26.0 |
94.7 |
14.9 |
35.4 |
14.2 |
31.6 |
33.6 |
12 |
10.5 |
28.6 |
96.8 |
21.0 |
56.3 |
20.6 |
54.5 |
55.4 |
16 |
12.0 |
30.2 |
97.4 |
24.9 |
69.1 |
24.4 |
66.9 |
68.0 |
19 |
13.6 |
31.9 |
97.9 |
27.5 |
74.5 |
27.0 |
72.3 |
73.4 |
22 |
14.4 |
32.7 |
97.9 |
28.5 |
75.5 |
28.1 |
73.9 |
74.7 |
26 |
17.0 |
35.4 |
98.4 |
31.3 |
76.6 |
31.0 |
75.5 |
76.1 |
28 |
18.5 |
36.9 |
98.4 |
32.5 |
75.0 |
32.5 |
75.5 |
75.3 |
Description of key information
Readily biodegradable: 75% (CO2 evolution) in 28 days (OECD 301B)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Studies investigating the ready biodegradability of Isotridecanol, ethoxylated, < 2.5 EO (CAS 69011-36-5) are not available. The assessment is therefore based on tests available for the structural analogue Isotridecanol, ethoxylated, 3 EO (CAS 24938-91-8). Three studies conducted according to OECD guideline 301B, conducted with domestic activated sludge as inoculum, are available.
In the key study, a degradation rate of 75% based on CO2 evolution was determined. Based on DOC removal a degradation rate of 82% was recorded after 28 days of incubation (OECD 301B, 1999). Based on the results the substance is readily biodegradable. In a supporting study, a degradation rate of 60.2 mg/L based on CO2 evolution was determined. The test substance can be considered as readily biodegradable but failing the 10-day window (OECD 301B, 2006). In a third study conducted according to OECD 301 B a degradation rate of 50.7% was determined (OECD 301b, 2005).
Based on the results for the read-across substance, Isotridecanol, ethoxylated, < 2.5 EO (CAS 69011-36-5) is considered readily biodegradable according to OECD criteria.
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