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EC number: 500-213-3 | CAS number: 68439-50-9 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01 Mar - 13 Mar 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations
- Sampling method: sampling at 0 h and 24 (fresh solutions) and after 48 h (aged solutions)
- Sample storage conditions before analysis: The samples were mixed with 10 mL acetonitrile and stored deep-frozen (≤ - 18 °C) until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution with a nominal concentration of 10 mg/L was prepared by adding the necessary amount of test item to a volumetric flask. Test medium was added and the solution was shaken and stirred for 60 minutes at 1000 rpm. The solution was observed to be clear and transparent; foam was observed. Test item concentrations were prepared by dilution with test medium. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Clone V
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: Daphnia magna was bred as single culture (1 daphnid per 100 mL) in Elendt M4 medium. pH-value: 6.0 – 9.0, dissolved oxygen: > 60 % saturation, total hardness 140 - 250 mg/L (as CaCO3), fed with single cell green algae (Desmodesmus subspicatus, formerly Scenedesmus subspicatus) at least three times a week.
- Source: bred at testing faciltiy, originally obtained from the Federal Environment Agency in Berlin/Germany.
- Feeding during test: none
ACCLIMATION
- Acclimation period: none - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/L CaCO3
- Test temperature:
- 19.6 – 20.4 °C
- pH:
- 7.73 – 7.95
- Dissolved oxygen:
- ≥ 8.03 mg/L
- Nominal and measured concentrations:
- Nominal test substance concentrations: 0.0625, 0.125, 0.250, 0.500, 1.00 mg/L
Time weighted mean concentrations: 0.0274, 0.0455, 0.0689, 0.116, 0.201 mg/L (based on 2 representative constituents) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers (nominal: 100 mL)
- Type (delete if not applicable): covered by glass plate
- Volume of solution: ≥ 50 mL
- Aeration: none
- Renewal rate of test solution (frequency): after 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Loading rate: ≥ 10 mL / animal
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: Temperature, pH-value and oxygen concentration were measured after 0, 24 hours (aged and fresh) and 48 hours
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light and 8 hours darkness
- Light intensity: 1181 ± 104 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mobility and behavioural changes 24 and 48 h
RANGE-FINDING STUDY
- Test concentrations: 0.010, 0.10, 1.00, 10.0 mg/L
- Results used to determine the conditions for the definitive study: EC50 (48 h) >0.10 mg/L < 1 mg/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.125 mg/L
- 95% CI:
- >= 0.11 - <= 0.142
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: based on two representative constituents
- Remarks:
- C12EO2 and C12EO3
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.045 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: based on two representative constituents
- Remarks:
- C12EO2 and C12EO3
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: After 24 hours 20% immobile daphnids were observed at a nominal test item concentration of 0.500 mg/L. 100% immobilisation was observed at a nominal test item concentration of 1.00 mg/L. After 48 hours 20% immobile daphnids were observed at nominal test item concentrations of 0.250 and 0.500 mg/L.
- Mortality of control: none
- Other adverse effects control: After 24 hours the mobile daphnids in the nominal test item concentration of 0.500 mg/L were sluggish. On immobile daphnids at nominal test item concentration 1.00 mg/L particles were observed. After 48 hours the mobile daphnids in the nominal test item concentrations of 0.250 and 0.500 mg/L were sluggish. On some immobile daphnids at nominal test item concentration 0.500 mg/L particles were observed.
- Immobilisation of control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The appearance of the test solutions was assessed daily and found clear and transparent from 0.0625 to 1.0 mg/L at each assessment - Results with reference substance (positive control):
- EC50 (24 h) of the reference item potassium dichromate: 0.6 - 2.1 mg/L (test started on 23 Feb 2021).
- Reported statistics and error estimates:
- The NOEC and LOEC were determined by using a multiple comparison method (Step-down Cochran-Armitage test procedure). The value for EC50 after 24 h was determined by Weibull analysis using linear max. likelihood regression. The EC50-value after 48 h was determined following the trimmed Spearman-Karber procedure.
- Validity criteria fulfilled:
- yes
- Remarks:
- Please see table 1 at "any other information on results incl. tables"
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 13 - 15 FEB 2008
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted 2004
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: An acetonic stock solution was prepared. The respective amounts of this stock solution were directly applied to the vessels and the acetone was evaporated. Subsequently test medium was added and the test solutions were moderately stirred overnight followed by filtration (glassfibre filter). The resulting water accomodated fractions wre used in the test. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: waterflea
- Length at study initiation (length definition, mean, range and SD): < 2 mm
- Method of breeding: parental and young Daphnia were held in 40 L glass aquaria at 20 +/- 2 °C.
ACCLIMATION
- Acclimation period: 4 h
- Type and amount of food: 15 g Sera micropan (Sera GmbH, D-52525 Heinsberg) homogenized in 1 L deionized water
- Feeding frequency: once a day, except weekends - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20 ± 0.5 °C
- pH:
- 6.7 - 7.9
- Dissolved oxygen:
- 5.9 - 6.4 mg/L
- Nominal and measured concentrations:
- Nominal test substance concentrations: 1.02, 2.56, 6.40, 16.0, 40.0 and 100 mg/L (WAF)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks (100 ml)
- Fill volume: 50 ml
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Intervals of water quality measurement: at test start and test end
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation after 24 and 48 h
RANGE-FINDING STUDY
- Test concentrations: 1, 10 an d100 mg/L
- Results used to determine the conditions for the definitive study: 65% immobile Daphnia after 48 h at 10 mg/L - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.49 mg/L
- 95% CI:
- >= 1.74 - <= 3.47
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.838 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Remarks:
- Please see table 1 at "any other information on results incl. tables"
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 22 - 24 JUN 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: EG-Guideline 92/69/EWG
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by diluting 20 mg/L in demineralised water. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: waterflea
-- Strain/clone: clone 5
- Source: in-house culture
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: none
ACCLIMATION
- Type and amount of food: Scenedesmus subspicatus ad libitum
- Feeding frequency: daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20 +/- 1 °C
- pH:
- 7.4 - 7.5
- Dissolved oxygen:
- 7.1 - 8.4 mg/L
- Nominal and measured concentrations:
- nominal: 0.1, 0.2, 0.4, 0.8, 1.6 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:glass beaker
- Fill volume: 10 mL test solution
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: one test organism per 2 mL test solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: CaCl2 x 2 H2O: 294 mgL; MgSO4 x 7 H2O: 123 mg/L, NaHCO3: 63 mg/L and KCl: 5.5 mg/L
- Ca/mg ratio: 4 : 1
- Intervals of water quality measurement: test start and test end
OTHER TEST CONDITIONS
- Photoperiod: continous darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility after 24 and 48 h - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.53 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.61 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- The reference substance was tested in a separate test (1993-04-20)
- Mortality: 0.9 mg/L - 30%; 1.9 mg/L - 100% - Validity criteria fulfilled:
- not specified
- Remarks:
- Please see table 1 at "any other information on results incl. tables"
- Conclusions:
- The 48-hour acute toxicity of alcohols, C12-14, ethoxylated (2 EO) to Daphnia magna, was determined in accordance with EU Guideline 92/69/EWG. The 48-hour EC50 value was determined to be 0.53 mg/L. The test is considered valid.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 09 - 11 DEC 2002
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 25 ml of each of the four stock solutions were mixed and made up to one litre with distilled water. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Age at study initiation (mean and range, SD): 6-24 h
- Source: Istituto Ricera sulle Acque, Brugherio, Italy
- Feeding during test : no
ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food: green algae (Selenastrum capricornutum) and yeast (Saccharomyces cerevisae)
- Feeding frequency: daily
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 180 mg CaCO3/L
- Test temperature:
- 21°C
- pH:
- 7.0
- Dissolved oxygen:
- 6.6 mg/L
- Nominal and measured concentrations:
- Nominal test substance concentration: 2 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers (100 ml)
- Fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: filtered tap water
OTHER TEST CONDITIONS
- Photoperiod: 12 h light/12 h darkness
- Light intensity: fluorescent light
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility after2, 4, 8, 24 and 48 h.
- Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Remarks:
- Please see table 1 at "any other information on results incl. tables"
Referenceopen allclose all
Table 1: Validity criteria for OECD 202 (2004)
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0% |
yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
≥ 8.03 mg/L |
yes |
Table 2: Results of the test, 48 h values
|
Nominal test item concentration [mg/L] |
|||||
|
Control |
0.0625 |
0.125 |
0.250 |
0.500 |
1.00 |
|
Immobilised daphnids after 48 h |
|||||
Replicate 1 |
0 |
0 |
0 |
1 |
2 |
5 |
Replicate 2 |
0 |
0 |
0 |
1 |
1 |
5 |
Replicate 3 |
0 |
0 |
0 |
1 |
1 |
5 |
Replicate 4 |
0 |
0 |
0 |
1 |
0 |
5 |
Σ |
0 |
0 |
0 |
4 |
4 |
20 |
% |
0 |
0 |
0 |
20 |
20 |
100 |
Table 1: Validity criteria for OECD 202
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
5 -10% |
yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
5.9 - 6.4 mg/L |
yes |
Table 2 Results
Nominal concentration |
Replicate |
Test period (h) |
|
24 h |
48 h |
||
Immobility [%] |
Immobility [%] |
||
0 |
A B C |
5 0 5 |
5 10 5 |
1.02 |
A B |
0 0 |
5 0 |
2.56 |
A B |
60 45 |
60 65 |
6.40 |
A B |
90 75 |
95 80 |
16.0 |
A B |
85 90 |
100 95 |
40.0 |
A B |
95 100 |
100 95 |
100 |
A B |
100 95 |
100 100 |
Table 1: Validity criteria for OECD 202
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0% |
yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
6.6 mg/L |
yes |
Table 2: Immobilisation
Nominal concentration |
Test period (h) |
|
24 h |
48 h |
|
Immobility [%] |
Immobility [%] |
|
Control |
0 |
0 |
0.1 |
0 |
0 |
0.2 |
0 |
0 |
0.4 |
10 |
20 |
0.8 |
75 |
95 |
1.6 |
100 |
100 |
Table 1: Validity criteria for OECD 202
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0% |
yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
6.6 mg/L |
yes |
Table 2: cummulative immobilisation
|
Observation time [h] |
||||
|
2 |
4 |
8 |
24 |
48 |
Control |
0 |
0 |
0 |
0 |
0 |
2 mg/L |
0 |
0 |
0 |
0 |
0 |
Description of key information
EC50 (48 h): 0.125 mg/L (TWA; OECD 202)
Key value for chemical safety assessment
Additional information
The key study, investigating the acute toxicity of Alcohols, C12-14, ethoxylated to Daphnia magna, was conducted according to OECD guideline 202. The Daphnids were exposed to nominal test substance concentrations of 0.0625, 0.125, 0.250, 0.500 and 1.00 mg/L for 48 hours. The test solutions were renewed every 24 h. the test concentrations were analytically verified by HPLC. Based on two representative constituents time weighted mean concentrations of 0.0274, 0.0455, 0.0689, 0.166, and 0.201 mg/L were determined. An EC50 (48 h) of 0.125 mg/L (TWA) was determined.
Three supporting studies are available. A static test following EG-Guideline 92/69/EWG determined an EC50 (48 h) of 0.53 mg/L (nominal). A static test conducted according to OECD guideline 202 determined an EC50 (48 h) of 2.49 mg/L (nominal). A second supporting study following OECD guideline 202 determined an EC50(48 h) > 2 mg/L (nominal) in a static limit test.
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