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EC number: 482-140-6 | CAS number: 13641-96-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- The test substance was considered reactive when added to water with potential gaseous products generated. Therefore, an alternative aeration strategy, to maximise exposure of the test substance to sludge, was considered appropriate.
- Qualifier:
- according to guideline
- Guideline:
- other: EC Directive 88/302, ‘Biodegradation - Activated Sludge Respiration Inhibition Test
- Principles of method if other than guideline:
- The test substance was considered reactive when added to water with potential gaseous products generated. Therefore, an alternative aeration strategy, to maximise exposure of the test substance to sludge, was considered appropriate, which involved vigorous shaking rather than air sparging.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 482-140-6
- EC Name:
- -
- Cas Number:
- 13641-96-8
- Molecular formula:
- Hill formula: C6 H7 N O3 CAS formula: C6 H7 N O3
- IUPAC Name:
- 2-isocyanatoethyl prop-2-enoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Information supplied by the Sponsor indicated that the test substance was a liquid that reacted with water. Therefore, in order to maximise test substance contact with the inoculum, the tests were conducted using two litre Erlenmeyer flasks and the contents aerated on an orbital
shaker for 3 hours at a constant speed of ca. 200 rpm. Test concentrations, based on the specific gravity of AOI (1.132), were established by the
addition of appropriate volumes of the test substance using an automatic pipette to flasks containing 284 mL RO water immediately before test set up. Additions of synthetic sewage and the inoculum were then made at fifteen minute intervals to give a final volume of 500 mL.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: No, A sample of activated sludge was obtained from Worlingworth Sewage Treatment Works, which treats predominantly domestic waste.
- Pretreatment: In the laboratory, the samples were maintained under aerobic conditions until required. Synthetic sewage (50 mL/L) was added to each stock of activated sludge and these were aerated overnight.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 19.9 - 20.9
- pH:
- 7.6 - 8.0
- Nominal and measured concentrations:
- Nominal concentrations: 6.25, 12.5, 25, 50, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size: two litre Erlenmeyer flasks
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reverse Osmosis (RO) water
- other: Synthetic sewage feed for activated sludge was prepared by dissolving the following in one litre of RO water:
peptone - 16.0 g
meat extract - 11.0 g
urea - 3.0 g
sodium chloride - 0.7 g
calcium chloride dihydrate - 0.4 g
magnesium sulphate heptahydrate - 0.2 g
di-potassium hydrogen phosphate - 2.8 g
OTHER TEST CONDITIONS
- Adjustment of pH: No - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (3,5-DCP)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 12.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 3.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50 for 3,5-DCP : 6.6 mg/L in the preliminary test and 7.3 mg/L in the definitive test.
Any other information on results incl. tables
Test substance:
Concentration-related inhibition of respiration rates occurred at each AOI concentration in the definitive test. The highest tested concentration of AOI in the definitive test (100 mg/l) caused 91% inhibition.
The EC50 of the test substance was calculated from the results of the definitive test to be 12.9 mg/l (95% confidence limits, 10.0 - 16.5 mg/l). The EC20 and EC80 values were calculated to be 3.9 and 43.3 mg/l (95% confidence limits, 2.2 – 5.9 and 31.5 – 63.5 mg/l).
Reference substance
The three-hour EC50 for 3,5-DCP (6.6 mg/l in the preliminary test and 7.3 mg/l in the definitive test) fulfilled the validity criterion relating to sensitivity to inhibition (acceptable EC50 range 5 to 30 mg/l), and that relating to the respiration rates in the control (variation not greater than 15%) was also satisfied.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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