Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 404-110-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-02-23 to 1989-02-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed according to relevant guidelines and compliant with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- (former 84/449/EWG)
- Deviations:
- no
- Remarks:
- according to OECD guideline 202, adopted 2004
- GLP compliance:
- yes
Test material
Reference
- Name:
- Unnamed
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared at 2g/l test item (0.2% solution). The dispersion of the test item occurred through ultrasonication. Test concentrations ranged from 1 to 1000 mg/L test item using a spacing factor of 1.8.
- Eluate: dilution water according to DIN 38412 Teil 11, 8.2
- Controls: yes
- Evidence of undissolved material (e.g. precipitate, surface film, etc): after 24h precipitation of test item particles was observed for all concentration levels.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUSS
- Source: Bundesgesundheitsamt, Berlin
- Method of breeding: in 2000 ml vessels at 20° C, aerated vessels (0.45 µm filter), water renewal three times a week.
- Food type during breeding: dry food, two times per day
- Age at the beginning of the experiment: not specified.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- Treatment groups: 22.2 °C
Controls: 22.2 °C - pH:
- Treatment groups: 7.71 - 7.92
Controls: 7.71 - Dissolved oxygen:
- Treatment groups: 7.9 - 8.1 mg/l
Controls: 8.1 mg/l - Nominal and measured concentrations:
- Nominal test item concentrations were achieved by dilution of a test item stock solution prepared at 2 g/l test item (0.2%).
Range of nominal concentrations: 1.0, 1.8, 3.2, 5.8, 10, 18, 32, 58, 100, 180, 320, 580, 1000 mg/l test item (spacing factor: 1.8) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml vessels (DIN 12 331)
- Fill volume: 20 ml
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: DIN 38412 Teil 11, 8.2
- Conductivity: <5 µS/cm
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12 h, diffuse
- Light intensity: 10 µE/qm *s,
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilization, after 24h and 48h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study: yes
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % CL could not be determined
- Details on results:
- No significant dose-response-curve could be generated: precipitation of test item particles occurred with respect to all concentrations after 24h. The particles adsorbed at the cuticle of the test organisms and hindered the daphnid's motility.
- Effect concentrations exceeding solubility of substance in test medium: yes
- Mortality of control: no
- Other adverse effects control: no - Results with reference substance (positive control):
- - Results with reference substance valid? Yes.
- EC0: 0.7 mg/l, EC50: 1,2 mg/l, EC100: 2.0 mg/l
- Other: Reference test has been carried out in parallel to the main test for 24h. - Reported statistics and error estimates:
- Applied statistics were according to "Ausgewählte Methoden der Wasseruntersuchung", Band II (1982), 379-382, VEB Fischer Verlag.
Any other information on results incl. tables
Test concentrations for the determination of effect concentrations
Conc. |
Immobilization |
Diss. Ox. |
pH |
Temp. |
|||
|
24h |
48h |
|
|
|
||
|
1. |
2. |
1. |
2. |
|
|
|
1000 |
40 |
30 |
40 |
40 |
7.9 |
7.85 |
20.2 |
580 |
40 |
50 |
50 |
60 |
7.9 |
7.87 |
„ |
320 |
40 |
40 |
60 |
60 |
7.8 |
7.86 |
„ |
180 |
20 |
30 |
30 |
30 |
7.9 |
7.85 |
„ |
100 |
20 |
30 |
50 |
40 |
8.1 |
7.84 |
„ |
58 |
40 |
20 |
50 |
30 |
8.0 |
7.83 |
„ |
32 |
20 |
40 |
20 |
40 |
7.9 |
7.81 |
„ |
18 |
10 |
0 |
20 |
20 |
8.0 |
7.80 |
„ |
10 |
10 |
0 |
30 |
10 |
8.1 |
7.80 |
„ |
5.8 |
0 |
10 |
20 |
20 |
7.9 |
7.92 |
„ |
3.2 |
0 |
0 |
20 |
10 |
8.0 |
7.85 |
„ |
1.8 |
0 |
0 |
0 |
0 |
8.1 |
7.80 |
„ |
1.0 |
0 |
0 |
0 |
0 |
8.0 |
7.77 |
„ |
Control |
0 |
0 |
0 |
0 |
8.1 |
7.71 |
„ |
The following effect concentrations have been derived:
EC0-24h: 3.2 mg/l
EC0-48h: 1.8 mg/l
EC50-24h: >1000 mg/l
EC50-48h: >500 mg/l
EC100-24h/48h: >1000 mg/l
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The derived EC50 (48h) for the immobilization of Daphnia magna was >500 mg/l.
- Executive summary:
In the present study the immobilization of Daphnia magna has been evaluated after 24h and 48h of exposure to the test item. The concentrations tested were prepared from a super-saturated solution prepared at 2 g/l test item using ultrasonication for dispersion. The nominal concentrations tested were as follows: 1.0, 1.8, 3.2, 5.8, 10, 18, 32, 58, 100, 180, 320, 580, 1000 mg/l test item. No analytical measurements were performed based on the very low solubility of the test item (<1µg/l, see section Physical and chemical properties).
After an exposure of 24h, precipitation of test item particles occurred in all test concentrations, which can be explained by the low water solubility of the test item (<1 µg/l, see section Physical and chemical properties). Furthermore, adsorption of the test item occurred on the cuticle of the test organisms hindering the motility of the organisms. Thus, observed immobility of Daphnia magna may be caused by the physical impact of undissolved test item particles instead of inherent toxicity. Summarizing, the following effect concentrations were determined:
EC0-24h: 3.2 mg/l
EC0-48h: 1.8 mg/l
EC50-24h: >1000 mg/l
EC50-48h: >500 mg/l
EC100-24h/48h: >1000 mg/l
The study was performed according to 84/449/EWG (later EU Method C.2 (Acute toxicity for Daphnia) and GLP.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.