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EC number: 291-716-4 | CAS number: 90459-71-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From July 01 to July 19, 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Octadecanoic acid, reaction products with triethylenetetramine, chloromethane-quaternized
- EC Number:
- 291-716-4
- EC Name:
- Octadecanoic acid, reaction products with triethylenetetramine, chloromethane-quaternized
- Cas Number:
- 90459-71-5
- Molecular formula:
- Not applicable - UVCB substance
- IUPAC Name:
- Octadecanoic acid, reaction products with triethylenetetramine, chloromethane-quaternized
- Test material form:
- solid
1
- Specific details on test material used for the study:
- Test item name: Octadecanoic acid, reaction products with triethylenetetramine, chloromethane-quaternized
Batch number: I1-0000196125
Manufacturing date: July 28, 2018
Expiry date : July 28, 2019
C.A.S. number: 90459-71-5
EEC number : 291-716-4
Molecular weight: UVCB substance
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- 0.01, 0.03, 0.10, 0.30 and 1.00 mg test item/L.
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The study was performed with the unicellular, fresh-water green algae Pseudokirchneriella subcapitata strain (formerly known as Selenastrum capricornutum), cultured in the laboratories of the Test Facility and originally purchased from the Institute of Plant Physiology of the University of Göttingen, Germany.
The algae were cultured in a climatic chamber at 24 °C ± 2 °C under continuous uniform illumination in the range 4440 - 8880 Lux in the spectral range 400-700 nm. The culture medium was the same of the test medium; the stock cultures were weekly transferred to fresh medium and maintained in continuous shaking to ensure the necessary amount of CO2 and to keep algae in suspension. Cultures containing deformed or abnormal cells were discarded. Only exponentially growing algal cultures have been used to start the test.
Study design
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 23.9 – 24.1 °C.
- pH:
- 7.93 – 7.96 at the test start
7.08 – 7.65 at the test end - Nominal and measured concentrations:
- The biological results were referred to nominal concentrations of test item.
The main final test included five nominal test item concentration (constant factor 3.2) corresponding to 0.01, 0.03, 0.10, 0.30 and 1.00 mg test item/L.
The test medium was prepared as follows:
prior to the test start, a 10.0 mg test item/L stock solution was prepared by direct weighting of 0.0050 g of test item into 500 mL of algal medium. The obtained solution appeared clear with particles if test item. This solution was sonicated for about five minutes and after was stirring with magnetic stirrer for about 2 hours. After stirring the stock solution appeared clear and transparent. Start by this stock solution the other concentrations were prepared by dilution as reported in the following table. - Details on test conditions:
- All the described procedures were conducted under a laminar flow bench to ensure the sterility of the algal cultures.
For the test concentrations and for the negative control, 100 mL capacity conical glass flasks capped with air permeable stoppers were used.
Three replicates for test concentrations and six for negative control were prepared. At test start, 50 mL of test solutions were poured into the test flasks and inoculated with algae to obtain a starting cell density of about 104 cells/mL.
The algal inoculum was taken from a pre-culture, started 3 days before the beginning of the test in order to ensure that the assay was performed with exponentially growing algal population. The cell density of the inoculum was around 106 cells/mL.
The test flasks were incubated in a climatic chamber, under continuous illumination and constant shaking. The flasks were randomly distributed under the light and were replaced every day during the test.
Light conditions:
continuous illumination; light intensity was ranged between 6340 and 6850 Lux, within the OECD recommended range (4440 - 8880 Lux). - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol pestanal
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.44 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.09 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.03 mg/L
- Nominal / measured:
- nominal
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxicity of test item Octadecanoic acid, reaction products with triethylenetetramine, chloromethane-quaternized was tested on Pseudokirchneriella subcapitata.
The 72-hour EC10, EC20 and EC50, the NOEC and LOEC, calculated in terms of the nominal concentrations of test item, for the two end-points, are reported in the following table:
Endpoint 0 - 72 h EC10 (mg/L) 0 - 72 h EC20 (mg/L) 0 - 72 h EC50 (mg/L) 0 - 72 h NOEC (mg/L) 0 - 72 h LOEC (mg/L)
Growth rate 0.08 (0.05 – 0.16)* 0.14 (0.07 – 0.23)* 0.44 (0.21 – 0.58)* 0.03 0.10
Yield** 0.03 (0.00 – 0.05)* 0.04 (0.02 – 0.07)* 0.09 (0.06 – 0.20)* 0.03 0.10
(*) 95% confidence limits.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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