5-amino-3-[[4-[[4-[[4-anilino-2-hydroxyphenyl]azo]phenyl]amino]-3-sulphophenyl]azo]-4-hydroxynaphthalene-2,7-disulphonic acid, sodium salt

Short-term toxicity to fish

Description of key information

Not harmful/toxic for short-term exposure to fish

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

117.7 mg/L

Additional information

The following data was obtained for the Similar Substance 01. It is expected that the Target substance will present similar effect levels to fish. Justification for the use of a read-across approach is provided in Section 13 of IUCLID.

The toxicity of the substance to fish was assessed in a static acute toxicity test using Zebrafish, according to the EU Method C.1. 10 fish per concentration were used. Fish were exposed to the following concentration, over a period of 96 hours: 56, 100, 178, 316 and 562 ppm. Water samples were taken from the bath at the beginning and at the end of the experiment and the tested concentrations resulted to be maintained at least 80 % of the nominal one, over the test period. No abnormal responses of the fish were observed. The LC0 at 96 hours resulted to be 100 ppm, while the LC100 resulted to be 178 ppm. The LC50 at 96 hours resulted to be 117.7 ppm and 116 ppm, based on geometrical mean and graphically determined, respectively.

A second test is available. The experiment was performed using five Zebrafish per test concentration, under semi-static conditions. Fish were exposed to three concentrations, i.e. 46, 64 and 100 mg/l. One death occurred at the concentration of 64 mg/l after 24 hours; at the highest concentration of 100 mg/l 3 fish died after 24 hours and the remaining fish died after 48 hours. The LC50 was determined to be 86.4 mg/l of active ingredient.