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EC number: 268-799-0 | CAS number: 68140-48-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 December 1993 to 15 December 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- The upper test concentration levels appear to exceed the aqueous solubility of the test item, which may have led to an insoluble fraction present in the exposure solutions. Two duplicate samples per test level with 10 daphnids each compared with 4 replicates of 5 daphnids each, typical of more recent studies. Report is missing additional information.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1-[2,3-dihydro-1,1,2,6-tetramethyl-3-(1-methylethyl)-1H-inden-5-yl]ethan-1-one
- EC Number:
- 268-799-0
- EC Name:
- 1-[2,3-dihydro-1,1,2,6-tetramethyl-3-(1-methylethyl)-1H-inden-5-yl]ethan-1-one
- Cas Number:
- 68140-48-7
- Molecular formula:
- C18H26O
- IUPAC Name:
- 1-[1,1,2,6-tetramethyl-3-(propan-2-yl)-2,3-dihydro-1H-inden-5-yl]ethan-1-one
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- TRASEOLIDE :
Batch / Sample Code : N47
Purity : 97% active ingredient
Date of Expiry : Not known
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- HPLC
- Details on sampling:
- Test substance concentrations were determined on Day -1, 0, 1 and 2.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Preparation of Stock and Test Solutions :
The stock solution used in this study was prepared by dispersing 0.02 g of Traseolide in 2 L of ASTM medium-hard by sonication for approximately 45 minutes to make a 10 mg/L stock solution.
Test solutions were made by adding known volumes of this stock to 2.0 L of ASTM medium-hard medium.
These test solutions were used to prepare the glassware for 3 days prior to the start of the test. A second stock was used to mprepare the test solutions for the definitive test. Test solutions were prepared 24 hours before the Daphnia were added to permit test substance equilibration.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna were cultured in the laboratory. Cultures were maintained in a synthetic medium with the same chemical composition and similar pH and dissolved oxygen concentration as that used to prepare the test solutions. Culture medium consists of 800 mL ASTM medium-hard medium with the addition of an organic additive, derived from the marine microalga Ascophyllum nodosum. The cultures are fed daily with cells of the green alga Chlorella vulgaris. Cultures are re-set using 10 neonates, when they become gravid their numbers are reduced from 10 to 5 and the production of neonates results in their daily removal.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Remarks:
- ASTM medium-hard medium
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Total Hardness betwee 165 - 184 mg/L as CaCO3
- Test temperature:
- Temperature range during exposure : 20.5 - 23.0 °C
- pH:
- pH range during exposure : 8.1 - 8.4
- Dissolved oxygen:
- Dissolved Oxygen range during erxposure : 7.2 - 9.0 mg/L
- Nominal and measured concentrations:
- The biological effects are, thus, expressed based on mean measured concentrations :
Nominal Concn (mg/L) Mean Measured Concn. (mg/L)
Control 0
0.10 0.14
0.18 0.15
0.32 0.22
0.56 0.32
1.0 0.50
1.8 0.90
3.2 1.92 - Details on test conditions:
- Exposure vessel : 100 mL glass crystallising dish
Duplicates : 2
No. of Neonates per Vessel : 10
Temperature : 20.5 - 23 °C
Light : Normal laboratory light intensity
Light / Dark Periods : 16 hour light / 8 hour dark
The tes solutions were prepared the day before the neonates were exposed to them. At the start of the test the Daphnids were added to the test vessels and watchglasses were placed over the test vessels to isolate the Daphnids and also to prevent them from coming into contact with the air-water interface.This avoids possible indirect effects from being caught in any surface film which might have developed. During the addition of the Daphnids, care was taken not to disturb the test medium. The exposure vessels were placed in the test area according to a random distribution. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.42 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limits : 0-4 - 0.5 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.22 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 1.92 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- A clear dose-response was observed.
Nominal Concn. Mean Msd. Concn. % Daphnids Immobile % Daphnids Immobile
(mg/L) (mg/L) after 24 hours after 48 hours
Control 0.0 0 10
0.1 0.14 0 5
0.18 0.15 5 5
0.32 0.22 5 10
0.56 0.32 5 15
1.0 0.50 20 85
1.8 0.90 45 85
3.2 1.92 50 100
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- EC50 (48 hour) = 0.42 mg/L (95% Confidence Limits : 0.4 - 0.5 mg/L)
EC0 (48 hour) = 0.22 mg/L
EC100 (48 hour) = 1.92 mg/L - Executive summary:
EC50 (48 hour) = 0.42 mg/L (95% Confidence Limits : 0.4 - 0.5 mg/L)
EC0 (48 hour) = 0.22 mg/L
EC100 (48 hour) = 1.92 mg/L
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