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EC number: 263-218-7 | CAS number: 61792-31-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 Jan - 28. Feb 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Adopted April 13, 2004
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-[3-(dimethylamino)propyl]dodecanamide N-oxide
- EC Number:
- 263-218-7
- EC Name:
- N-[3-(dimethylamino)propyl]dodecanamide N-oxide
- Cas Number:
- 61792-31-2
- Molecular formula:
- C17H36N2O2
- IUPAC Name:
- N-[3-(dimethylamino)propyl]dodecanamide N-oxide
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Control and the following test item concentrations: 32; 56 and 100 mg/L
- Sampling method: Single samples (2 mL) were taken after 0 and 48 h
- Sample storage conditions before analysis: Test samples were stored in the freezer (≤ -15°C).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Preparation of test solutions started with the highest concentration of 100 mg/L (i.e. 340 mg test item/L) applying approximately 3 to 3¼ hours of magnetic stirring to completely dissolve the test item in test medium. Lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium. All test solutions were clear and colorless at the end of the preparation procedure. Note that the appearance of the highest test concentration was not recorded directly after preparation.
- Controls: Medium without test item
- Evidence of undissolved material: No, during the test period it was clear and colourless
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Straus
- Age at study initiation (mean and range, SD): Daphnia, less than 24 hours old
- Method of breeding: Approx. 250 newborn daphnids in 5 L of medium, after 7 days half of the medium was renewed, daily feeding, max age of cultures: 4weeks, Temp: 18-22 °C, Culture medium: M7
- Source: In-house laboratory culture with a known history
- Age of parental stock: More than two weeks old, at least third generation, obtained by a cyclical parthenogenesis under specified breeding conditions
- Feeding during test : No
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES, INCLUDING CULTURING CONDITIONS: Daphnia, less than 24 hours old, from parental daphnids of more than two weeks old.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/L (CaCO3)
- Test temperature:
- 20 - 21 °C
- pH:
- 8.0 - 8.1 (Control)
7.8 - 8.1 (Test item concentrations) - Dissolved oxygen:
- 8.9 - 9.6 mg/L (Control)
7.4 - 9.7 mg/L (Test item concentrations) - Nominal and measured concentrations:
- Control, 10, 18, 32, 56 and 100 mg/L (nominal)
Since the measured test item cocnentration were within a range of 80 to 120% of nominal the toxicological endpoints were evaluted based on the nominal test item concentrations.
The measured test item concentrations were determined at test end (48h): 33.4 (32), 63.4 (56) and 107 (100) mg/L (nominal test item concentrations). - Details on test conditions:
- TEST SYSTEM
Test vessel:
- Type: open
- Material, size, headspace, fill volume: 60 mL all-glass test vessels, 10 mL headspace
- Volume of solution: 50 mL of test solution
- No. of organisms per vessel: 5 daphnids per vessel, 20 per test group
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: At least 2 mL of test solution per daphnid
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water purified by Reverse Osmosis (RO-water, GEON Waterbehandeling, Berkel-Enschot, The Netherlands), additionally following salts were added (analytical grade):
CaCl2 x 2H2O: 211.5 mg/L; MgSO4x7H2O: 88.8 mg/L; NaHCO3: 46.7 mg/L; KCl: 4.2 mg/L
- Culture medium different from test medium: Yes
- Intervals of water quality measurement: pH and oxygen concentration were measured at test start and test end
OTHER TEST CONDITIONS
- Photoperiod: 16 h
EFFECT PARAMETERS MEASURED: Immobility
RANGE-FINDING STUDY
- Test concentrations: Control, 0.1; 1.0; 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: No immobility was observed in the control and at the three lowest test concentrations throughout the test period. Complete immobility was found at the highest test concentration after 48 h. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 71 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95%-confidence interval
- Remarks:
- 65 - 76
- Details on results:
- - Immobilisation of control:
No immobility was observed in the control
- Reported statistics and error estimates:
- The 48h-EC50 value was calculated by applying the Spearman-Karber procedure (non-linear; without trimming) on the percentages of affected daphnids and the logarithms of the corresponding nominal test item concentrations. ToxRat Professional v 3.2.1 (ToxRat Solutions® GmbH, Germany) was used to perform the analyses.
Any other information on results incl. tables
Biological Result
Tabe 1: Number of Introduced Daphnids and Incidence of Immobility in the Final Test
Time (h) |
Replicate |
N-[3-(dimethyloxidoamino)propyl]-dodecanamide Nominal concentration (mg/L) |
|||||
|
|
Control |
10 |
18 |
32 |
56 |
100 |
0 |
A |
5 |
5 |
5 |
5 |
5 |
5 |
|
B |
5 |
5 |
5 |
5 |
5 |
5 |
|
C |
5 |
5 |
5 |
5 |
5 |
5 |
|
D |
5 |
5 |
5 |
5 |
5 |
5 |
Total introduced |
|
20 |
20 |
20 |
20 |
20 |
20 |
24 |
A |
0 |
0 |
0 |
0 |
1 |
5 |
|
B |
0 |
0 |
0 |
0 |
0 |
4 |
|
C |
0 |
0 |
0 |
0 |
0 |
5 |
|
D |
0 |
0 |
0 |
0 |
1 |
4 |
Total immobilised |
|
0 |
0 |
0 |
0 |
2 |
18 |
Effect % |
|
0 |
0 |
0 |
0 |
10 |
90 |
48 |
A |
0 |
0 |
0 |
0 |
1 |
5 |
|
B |
0 |
0 |
0 |
0 |
0 |
5 |
|
C |
0 |
0 |
0 |
0 |
0 |
5 |
|
D |
0 |
0 |
0 |
0 |
1 |
5 |
Total immobilised |
|
0 |
0 |
0 |
0 |
2 |
20 |
Effect % |
|
0 |
0 |
0 |
0 |
10 |
100 |
Analytical Results:
Samples taken from the nominal concentrations of 32, 56 and 100 mg/L showed that the measured concentrations were in agreement with nominal at the start of the test, i.e. were 109 -113%, and remained stable during the test period, i.e. were 104-113% of nominal at the end of the test. Small test item responses were detected in the control samples. The maximum contribution to the 32 mg/L sample ranged between 0.0012 and 0.00054%. Therefore, the contribution was not considered to affect the study results.
In view of these results, effect parameters were expressed in terms of analytically confirmed nominal concentrations.
Table 2: Validity criteria
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0 |
Yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
7.4 – 8.9 mg/L |
Yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”
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