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EC number: 251-780-6 | CAS number: 33996-33-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-07-03 to 2017-08-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004-04-13
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 2008-05-30
- Qualifier:
- according to guideline
- Guideline:
- other: SANCO/3029/99 rev.4 11/07/00: Residues: Guidance for generating and reporting methods of analysis in support of pre-registration data requirements for Annex II (part A; Section 4) and Annex III (part A; Section 5) of directive 91/414
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Nippon Rika Co., Ltd. / lot 6716-16E
- Expiration date of the lot/batch: 2018-10-23
- Purity test date: 2016-11-17
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At 20°C +/- 5°C, in the dark
- Solubility and stability of the test substance in the solvent/vehicle: highly soluble - Analytical monitoring:
- yes
- Details on sampling:
- The samples were taken from the biological phase of the study.
Duplicate samples from the freshly prepared test medium of the only test concentration and the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentration during the test period, duplicate samples from the test medium of the only test concentration and the control were collected at the end of the test (after 48 hours).
All samples were diluted by a factor of 2 with acetonitrile.
Additional samples of the control and the dilution solvent were taken at test start and test end without any sample treatment.
All samples were stored in a freezer (≤ - 20 °C), protected from light until analysis was performed. Afterwards the samples were again stored deep frozen (≤ -20 °C) and were kept stored up to the date of the final report.
The concentrations of the test item Oxaceprol were analysed in the duplicate test media samples from all test concentrations, and in the duplicate control samples, from both sampling times (0 and 48 hours). - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test item was weighed and dissolved
- Controls: yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): reconstituted water Elendt "M4"
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s)
including control(s)): 100 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): none - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: clone 5
- Source: ibacon
- Feeding during test: at least on all working days
- Food type: green algae (Desmodesmus subspicatus)
- Frequency: at least on all working days - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 2.5 mmol/L (= 250 mg/L) as CaCO3
- Test temperature:
- 20.8 to 20.9 °C at test start
20.1 to 20.3 °C at test end - pH:
- 7.7 to 8.4 at test start
7.8 at test end - Dissolved oxygen:
- 8.9 to 9.2 mg/L at test start
9.2 to 9.4 mg/L at test end - Nominal and measured concentrations:
- Nominal: 100 mg/L
At the start of the test 109% of the nominal test concentrations were found (of the nominal concentration of 100 mg test item/L). After 48 hours test duration, 114% of the nominal value was determined (of the nominal concentration of 100 mg test item/L). During the test the daphnids were exposed to a mean of 112% of nominal. Therefore, all reported results refer to nominal concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Glass beakers of 100 mL volume containing approximately 60
mL of test medium. The test vessels were covered with a lid to reduce the loss of water due to evapor
ation and to avoid the entry of dust into the solutions.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates): 4 per treatment group
- No. of vessels per vehicle control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
Analytical grade salts and additives were added at the following nominal concentrations in deionised
water (conductivity < 5 μScm-1):
Main compounds:
CaCl2·2 H2O 293.80 mg/L
MgSO4·7 H2O 123.30 mg/L
KCl 5.80 mg/L
NaHCO3 64.80 mg/L
Na2SiO3·9 H2O 10.00 mg/L
NaNO3 0.27 mg/L
KH2PO4 0.14 mg/L
K2HPO4 0.18 mg/L
Trace elements:
H3BO3 2860 μg/L
MnCl2·4 H2O 361 μg/L
LiCl 306 μg/L
RbCl 7 1 μg/L
SrCl2·6 H2O 152 μg/L
NaBr 16 μg/L
Na2MoO4·2 H2O 63 μg/L
CuCl2·2 H2O 17 μg/L
ZnCl2 13 μg/L
CoCl2·6 H2O 10 μg/L
KI 3.3 μg/L
Na2SeO3 2.2 μg/L
NH4VO3 0.6 μg/L
Na2EDTA·2 H2O 2.5 mg/L
FeSO4·7 H2O 1.0 mg/L
Vitamins:
Thiamin HCl 75.0 μg/L
Cyanocobalamin (B12) 1.0 μg/L
Biotin (B6) 0.75 μg/L
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
- Light intensity: 500 - 670 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
VEHICLE CONTROL PERFORMED: yes. Most recent Test with the Reference Item Potassium dichromate performed in January 2017 - Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50, EC20, EC10, NOEC, LOEC
- Limit test: no
- Dose-response test: yes
- ECx: EC50 (48 h) = 1.06 mg/L; EC20 (48 h) = 0.967 mg/L; EC10 (48 h) = 0.922 mg/L
- Other: NOEC (48 h) = 0.5 mg/L; LOEC (48 h) = 1.0 mg/L - Reported statistics and error estimates:
- Statistical analysis was not necessary.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Oxaceprol did not exhbit aquatic toxicity to Dahnia magna at a test concentration of 100 mg/L under the conditions of the test according to OECD 202.
- Executive summary:
The toxic effect of the test item Oxaceprol to Daphnia magna was assessed in a static limit test. The 48-hour NOEC was determined to be ≥ 100 mg test item/L. The 48-hour LOEC was determined to be > 100 mg test item/L and the 48-hour EC50 value was determined to be > 100 mg test item/L.
The quantification of the test item Oxaceprol in the test samples was performed using liquid chromatography with MS/MS detection.
At the start of the test 109% of the nominal test concentrations were found (of the nominal concentration of 100 mg test item/L). After 48 hours test duration, 114% of the nominal value was determined (of the nominal concentration of 100 mg test item/L). During the test the daphnids were exposed to a mean of 112% of nominal. Therefore, all reported results refer to nominal concentrations.
Oxaceprol did not exhbit aquatic toxicity to Dahnia magna at a test concentration of 100 mg/L under the conditions of the test according to OECD 202.
Reference
Description of key information
Oxaceprol did not exhbit aquatic toxicity to Dahnia magna at a test concentration of 100 mg/L under the conditions of the test according to OECD 202.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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