Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 241-659-6 | CAS number: 17675-60-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 April 2017 / 13 September 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- July 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 16VL8189
- Expiration date of the lot/batch: 003 August 2017
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: ambient (=< 30 deg C), dark, dry
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: No - Analytical monitoring:
- not required
- Details on sampling:
- Not applicable
- Vehicle:
- no
- Details on test solutions:
- Limit test: A weight of 499.3 – 501.1 mg of the test item was added directly into the test vessels 7 - 11 with a test volume of 500 mL to obtain a final concentration of 998.6 – 1002.2 mg/L. The pH of the test solution was measured before addition of inoculum. Full test: A Stock solution with 20 g/L test item was prepared (0, 2, 4, 7.2, 14.4 and 25.92 mL test item in stock solution). Five replicates of each concentration were prepared (80.0, 160.0, 288.0, 576.0 and 1037.0 mg/L). The pH of the test solutions were measured before addition of inoculum.
Reference item
Limit test: A weight of 24.8 mg of 3,5-dichlorophenol were dissolved in ultrapure water and filled up to 25 mL using a magnetic stirrer to get a stock solution with 1.0 g/L. 0.5, 5 and 15 mL of the stock solution were added to the reference vessels with a test volume of 500 mL in order to obtain final concentrations of 1, 10 and 30 mg/L.
Full test: A weight of 49.9 mg of 3,5-dichlorophenol were dissolved in deionised water and filled up to 50 mL using a magnetic stirrer to get a stock solution with 1.0 g/L. 0.5, 5 and 15 mL of the stock solution were added to the reference vessels in order to obtain final concentrations of 1, 10 and 30 mg/L. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge from the municipal wastewater treatment plant Staufener Bucht was used as test system. The treatment plant has a capacity of 140 000 inhabitant equivalents and clarifies predominantly domestic wastewater. Sampling date of activated sludge was 26 April 2017 (limit test) and 29 May 2017 (full test). The activated sludge was kept aerated until use at test temperature. Dry solid of the activated sludge was determined as 3.8 g/L (limit test) and 4.4 g/L (full test) by weight measurements before and after drying at 105°C (mean of triplicate measurements). The activated sludge was washed twice by settling the sludge, decanting the supernatant and re-suspending the sludge in chlorine-free tap water. Before using, the activated sludge was diluted to 3 g/L dry solids with chlorine-free tap water, in order to obtain a final concentration of 1.5 g/L dry solids in the test.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- None
- Post exposure observation period:
- Not applicable
- Hardness:
- Not measured
- Test temperature:
- Limit test: 19.7 – 21.8°C.
Full test: 21.4 - 21.8°C - pH:
- Limit test: 7.0 – 7.1. (adjusted)
Full test: 6.2- 7.1 (measured), 7.0 - 7.2 (adjusted) - Dissolved oxygen:
- 5.78 - 8.36 mg/L
- Salinity:
- Not applicable
- Conductivity:
- Not measured
- Nominal and measured concentrations:
- Limit test: 1000 mg/L
Full test: 80.0, 160.0, 288.0, 576.0 and 1037.0 mg/L - Details on test conditions:
- Experimental set up
The different vessels werestarted separately with an interval of exactly 15 min in order to be able to measure the respiration rates one by one while making sure that the aeration time is 3 h in every vessel. At time "0", 16 ml synthetic sewage and the test item or reference compound were added to the test vessel and made up to 250 mL with chlorine-free tap water. After measurement and adjustment of pH, 250 mL of activated sludge (3 g/L dry solids) were added consequently obtaining a total volume of 500 ml with 1.5 g/L dry solids. For the controls the same procedure was made as with the test vessels, however without test or reference substance. All vessels were stirred and aerated throughout the contact time with room air to assure an oxygen saturation of 60-70% using a membrane pump. Before starting the test the oxygen saturation rate was measured.
After 3 h the aeration of the first vessel was shut off and the respiration rate was measured in a BOD-bottle with an oxygen electrode while mixing with a magnetic stirrer. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 145.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other:
- Remarks:
- 95%-CL: 1.175 – 15679.727 mg/L
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 80 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- Limit Test
A limit test with the concentration of 1000 mg/L was performed. The pH in the test vessels was between 6.2 and was adjusted to 7.0 – 7.1.
An inhibition of the total respiration of activated sludge between 62 – 75% was observed in the test vessels with 1000 mg/L test item concentration. In accordance with the sponsor a full test with five concentrations was prepared afterwards.
The respiration rate of six blanks were within 31.2 – 54.7 mg/(L*h) that is equivalent to 20.8 – 36.5 mg/(L*h) per one gram activated sludge. The coefficient of variation (CV) was 19% and therefore less than 30%. The pH in the blank vessels was 7.3.
The temperature range within the test duration was between 19.7 – 21.8°C.
Test item
The inhibiting effect of the test item on the total respiration of activated sludge was observed with five concentrations (80.0, 160.0, 288.0, 576.0 and 1037.0 mg/L).
EC50, EC10, LOEC and NOEC are given by ToxRat Professional Version 3.2.1, © ToxRat Solutions GmbH
The results for the inhibition of the total respiration of activated sludge are therefore:
EC50 = 145.4 mg/L (95%-CL: 1.175 – 15679.727 mg/L)
EC10 = 0.603 mg/L (95%-CL: 0.011 – 31.807 mg/L)
LOEC <= 80.0 mg/L
NOEC < 80.0 mg/L
Blanks
The respiration rate of six blanks were within 19.2 – 32.0 mg/(g sludge*h). The coefficient of variation (CV) was 21.3% and therefore less than 30%. The pH in the blank vessels was between 7.3 – 7.4.
The temperature range within the test duration was between 21.4 and 21.8°C.
Criteria met for the validity of the study
The coefficient of variation (CV) of the blank respiration rates was less than 30%
The blank controls oxygen uptake rate was higher than 20 mg oxygen per one gram of
activated sludge per hour The EC50 of the reference item was within the acceptable range of 2 to 25 mg/l
- Results with reference substance (positive control):
- In the limit test the EC50 of 3,5-dichlorphenol was calculated as 4.10 mg/l and was therefore within the acceptable range of 2 to 25 mg/l.
In the full test the EC50 of 3,5-dichlorphenol was calculated as 3.047 mg/l (Appendix III) and was therefore within the acceptable range of 2 to 25 mg/l. The pH in the reference vessels was 7.4. - Validity criteria fulfilled:
- yes
- Conclusions:
- Limit test with test concentration 1000 mg/L:
An inhibition between 62 and 75% was observed in the test vessels with 1000 mg/L test concentration (five replicates).
Full test with five concentrations (80.0, 160.0, 288.0, 576.0 and 1037.0 mg/L):
EC50 = 145.4 mg/L (95%-CL: 1.175 – 15679.727 mg/L)
LOEC <= 80.0 mg/L
NOEC < 80.0 mg/L
(ToxRat Professional Version 3.2.1, © ToxRat Solutions GmbH) - Executive summary:
An OECD 209 Activated sludge inhibition test (2010) was conducted with the test item. This was to assess the inhibition effect of different concentrations of test item (80.0, 160.0, 288.0, 576.0 and 1037.0 mg/L) to micro-organisms of activated sludge by measuring the respiration rate after adding a standard amount of synthetic sewage feed. The test met all guideline validity criteria.
The results for the inhibition of the total respiration of activated sludge were:
EC50 = 145.4 mg/L (95%-CL: 1.175 – 15679.727 mg/L)
EC10 = 0.603 mg/L (95%-CL: 0.011 – 31.807 mg/L)
LOEC <= 80.0 mg/L
NOEC < 80.0 mg/L
Reference
Description of key information
An OECD 209 Activated sludge inhibition test (2010) was conducted with the test item. This was to assess the inhibition effect of different concentrations of test item (80.0, 160.0, 288.0, 576.0 and 1037.0 mg/L) to micro-organisms of activated sludge by measuring the respiration rate after adding a standard amount of synthetic sewage feed. The test met all guideline validity criteria.
The results for the inhibition of the total respiration of activated sludge were:
EC50 = 145.4 mg/L (95%-CL: 1.175 – 15679.727 mg/L)
EC10 = 0.603 mg/L (95%-CL: 0.011 – 31.807 mg/L)
LOEC <= 80.0 mg/L
NOEC < 80.0 mg/L
Key value for chemical safety assessment
- EC50 for microorganisms:
- 145.4 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.