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EC number: 229-129-2 | CAS number: 6416-66-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- other: expert judgement based on available data
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- accepted calculation method
- Guideline:
- other: REACH Guidance on QSARs R.6
- Inoculum or test system:
- other: expert judgement
- Parameter:
- probability of ready biodegradability (QSAR/QSPR)
- Remarks on result:
- not readily biodegradable based on QSAR/QSPR prediction
- Validity criteria fulfilled:
- not applicable
- Remarks:
- estimation by EPI Suite and QSAR
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Based on prediction carried out with EPI Suite and QSAR, the substance resulted as non ready biodegradable.
- Executive summary:
Method
Estimation of probability of rapid biodegradation relying on EPI Suite and QSAR dyes. The overall assessment is based on the responses given by different predictive models for biodegradability under various conditions: linear and non-linear models, ultimate and primary survey models, MITI linear and non-linear models, anaerobic linear model.
Results
The substance was predicted to be not ready biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Remarks:
- prediction
- Type of information:
- (Q)SAR
- Adequacy of study:
- supporting study
- Study period:
- 2020
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- accepted calculation method
- Justification for type of information:
- QSAR estimation
- Qualifier:
- no guideline required
- Principles of method if other than guideline:
- QSAR dyes R&C report produced by version QSAR dyes RC 2.0 Developed by Milano Chemometrics and QASR research group Dept. Earth and Environmental Science, University Milano
Bicocca, Italy. - Parameter:
- probability of ready biodegradability (QSAR/QSPR)
- Remarks on result:
- not readily biodegradable based on QSAR/QSPR prediction
- Validity criteria fulfilled:
- not applicable
- Remarks:
- estimation by prediction tool
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Based on prediction carried out with the prediction tool, the substance resulted as non ready biodegradable.
- Executive summary:
Results
The substance was predicted to be not ready biodegradable.
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- From the 15th of July to the 31th of August, 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Internal method SAV: 13 000 03
- GLP compliance:
- yes (incl. QA statement)
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Sludge from Ara Reinach communal sewage treatment plant, collected on 02/08/1994
- Duration of test (contact time):
- 28 d
- Details on study design:
- TEST CONDITIONS- Test temperature: 22.0 ± 3°C (room temperature)- Lighting: indirect daylight- Test concentrations: 150 mg/L DOC nominal
- Reference substance:
- diethylene glycol
- Preliminary study:
- A preliminary study was performed
- Parameter:
- other: DOC
- Value:
- 5.3
- Sampling time:
- 28 d
- Remarks on result:
- other: everage of 2 tests running in parallel
- Parameter:
- other: adsorption
- Value:
- 6.3
- Sampling time:
- 3 h
- Details on results:
- The biodegradation value has been corrected by the blank control.See table 1 attached for further details on data.
- Results with reference substance:
- DOC (mg/l) is: 99.5 % 8th day (average of 2 tests running in parallel).Corrected by the blank control and measured.
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- The biodegradation of the test substance Acid Red 249 after 28 days was 5.3 % while the biodegradation of the reference substance after 8 days was 99.5 %.
- Executive summary:
The inherent biodegradability of the substance was determined in a Zahn-Wellens, test according to the OECD Guideline for Testing of Chemicals, No. 302B, Paris 1992. The test has been performed in compliance with the Procedures and Principles of Good Laboratory Practice (March 1986) issued by the Swiss Federal Department of the Interior recognizing the " OECD Principles of Good Laboratory Practice " Paris, 1981. The test substance Acid Red 249 and the reference substance Diethylenglycol, respectively, were tested in concentrations of 150 mg/l DOC.
The biodegradation of the test substance after 28 days was 5.3 % while the biodegradation of the reference substance after 8 days was 99.5 %.
- Endpoint:
- biodegradation in water: screening tests
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- The 13th of September 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test not performed under GLP Guideline. Internal method were used, but not detailed.
- Reason / purpose for cross-reference:
- reference to other study
- Principles of method if other than guideline:
- Internal Method A.16
- GLP compliance:
- no
- Remarks:
- Pre GLP.
- Inoculum or test system:
- not specified
- Remarks on result:
- not determinable
- Parameter:
- BOD5
- Value:
- ca. 0 mg O2/g test mat.
- Key result
- Parameter:
- BOD5*100/COD
- Value:
- ca. 0 other: n.a.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- BOD of the test substance is 0 mg/g O2. The calculated Biodegradability index" (B.I.)., BOD5/COD is 0.
- Executive summary:
BOD5 was measured according to Method A-15. Details of the method have been not reported.
The BOD measured was 0 mg/g O2, at concentration of 500 mg/L.
The calculated Biodegradability index" (B.I.)., BOD5/COD is 0.
- Endpoint:
- biodegradation in water: screening tests
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- The 13th of September 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test not performed under GLP Guideline. Internal method were used, but not detailed.
- Reason / purpose for cross-reference:
- reference to other study
- Principles of method if other than guideline:
- Internal method A.16
- GLP compliance:
- no
- Remarks:
- Pre GLP.
- Inoculum or test system:
- not specified
- Remarks on result:
- not determinable
- Parameter:
- COD
- Value:
- ca. 1 087 mg O2/g test mat.
- Key result
- Parameter:
- BOD5*100/COD
- Value:
- 0
- Validity criteria fulfilled:
- not specified
- Conclusions:
- COD of the test substance is 1087 mg/g O2. The calculated Biodegradability index" (B.I.)., BOD5/COD is 0.
- Executive summary:
COD was measured according to Method A-16. Details of the method have been not reported.
The COD measured was 1087 mg/g O2, at concentration of 5 mg/L.
The calculated Biodegradability index" (B.I.)., BOD5/COD is 0.
Referenceopen allclose all
Concentration: 500 mg/L
Concentration: 5 mg/L
Description of key information
Based on expert judgement and QSAR prediction the substance is not readily biodegradable.
Additional tests for the evaluation of inherent biodegradability and BOD/COD evalutaion of the substance itself are available with weight of evidence approach.
Inherent biodegradability: the
biodegradation of the test substance after 28 days was 5.3 % while the
biodegradation of the reference substance after 8 days was 99.5 %.
COD: 1087 mg/g O2, at concentration of 5 mg/L.
BOD5: 0 mg/g O2, at concentration of 500 mg/L.
BOD5/COD: 0
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
Additional information
Based on expert judgement and QSAR prediction the substance is not readily biodegradable.
Different available tests may be valuable, as part of a Weight of Evidence approach that gives information on biodegradability.
Inherent biodegradability
The inherent biodegradability was determined in a Zahn-Wellens, test according to the OECD Guideline for Testing of Chemicals, No. 302B, Paris 1992. The test has been performed in compliance with the Procedures and Principles of Good Laboratory Practice (March 1986) issued by the Swiss Federal Department of the Interior recognizing the " OECD Principles of Good Laboratory Practice " Paris, 1981. The test substance and the reference substance Diethylenglycol, respectively, were tested in concentrations of 150 mg/l DOC.
The biodegradation of the test substance after 28 days was 5.3 % while the biodegradation of the reference substance after 8 days was 99.5 %.
COD/BOD5 ratio
Biochemical Oxygen Demand (BOD5) and Chemical Oxygen Demand (COD) are the most commonly used parameters for the characterization of the biodegradability capacity.
COD results are typically higher than BOD5 values, and the ratio between vary depending on the characteristics of the substrate. This ratio has been commonly used as an indicator for biodegradation capacity, the "Biodegradability index" (B.I.).
Studies on BOD5 and COD are available for this substance, therefore they have been used together with the inherent biodegradability OECD 302.B.
in the weight of evidence approach for this substance.
COD was measured according to Method A-16. Details of the method have been not reported. The COD measured was 1087 mg/g O2, at concentration of 5 mg/L.
BOD5 was measured according to Method A-15. Details of the method have been not reported. The BOD5 measured was 0 mg/g O2, at concentration of 500 mg/L.
The ratio BOD5/COD is 0.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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