Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 225-035-0 | CAS number: 4621-04-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study completion Date 15 May 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 4-isopropylcyclohexanol
- EC Number:
- 225-035-0
- EC Name:
- 4-isopropylcyclohexanol
- Cas Number:
- 4621-04-9
- Molecular formula:
- C9H18O
- IUPAC Name:
- 4-(propan-2-yl)cyclohexan-1-ol
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- For the determination of the appropriate test concentrations for the main test, a range-finding test was performed. The nominal test item concentrations tested were 1.0, 10 and 100 mg/L. For this, two replicates per treatment were tested under identical exposure conditions than those applied for the main test. The test medium of the highest concentration of 100 mg/L was stirred until dissolution for 10 minutes at room temperature. The test media of the two lower concentrations were prepared in a series of further dilution steps.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Based on the range-finder results the following nominal concentrations of FOLROSIA were selected for the main test: 4.6, 10, 22, 46 and 100 mg/L. Additionally, a control (test water without test item) was tested in parallel. For each treatment, 20 daphnids were randomly distributed into four replicates of five daphnids each. The volume of test medium provided for each daphnid was 10 mL (50 mL per replicate). Thus, the requirement of the test guidelines for a minimum volume of 2 mL test medium per daphnid was fulfilled.
The measured concentrations of the test item FOLROSIA in the test media of the nominal test concentrations of 4.6 to 100 mg/L were between 102 and 114% of the nominal values at the start of the test. Thus, the correct dosage of the test item could be verified. At the end of the test, 69 to 93% of the nominal values were found. The mean measured exposure concentration (geometric mean of Time 0 and 48 hour analyses) was 4.5, 9.4, 21, 43 and 86 mg/L, respectively, at the 4.6, 10, 22, 46 and 100 mg/L (nominal) test concentrations.
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- static
- Water media type:
- other: Reconstituted test water according to ISO 6341
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- The test was performed in a temperature-controlled room with continuous monitoring of the room temperature. The water temperature was in the range of 20-21 °C.
- pH:
- pH in the range of 7.6 to 7.8.
- Dissolved oxygen:
- Dissolved O2 was > or = 8.2 mg/L.
- Nominal and measured concentrations:
- The mean measured exposure concentration (geometric mean of Time 0 and 48 hour analyses) was 4.5, 9.4, 21, 43 and 86 mg/L, respectively, at the 4.6, 10, 22, 46 and 100 mg/L (nominal) test concentrations.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 42 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI : 35 - 50 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 9.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Series of sub-lethal effects observations (e.g. but not limited to discolouration, swimming behaviour)
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 9.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 86 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- For evaluation of the quality of the daphnia clone and the experimental conditions, potassium dichromate is tested as a positive control twice a year. The result of the latest positive control test in October 2016 (24-hour EC50: 1.0 mg/L, IES Laboratories Study 20160278) showed that the sensitivity of the test organisms was within the range given by the guideline (24-hour EC50: 0.60 2.1 mg/L).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
A clear dose-response effect was observed during the study. The test item FOLROSIA had acute toxic effects on Daphnia magna in a 48-hour static test.
The biological results were based on the geometric mean measured concentrations:
EC50 (48 hr): 42 (95% CI : 35 - 50) mg/L
EC0 (48 hr): 9.4 mg/L
EC100 (48 hr): 86 mg/L
NOEC (48 hr) : 9.4 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.