Registration Dossier
Registration Dossier
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EC number: 935-211-5 | CAS number: -
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07.02.2011 - 10.03.2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- phosphoric acid 6-(diphenoxy-phosphoryloxy)-hexahydro-furo[3,2-b]furan-3-yl ester diphenyl ester
- IUPAC Name:
- phosphoric acid 6-(diphenoxy-phosphoryloxy)-hexahydro-furo[3,2-b]furan-3-yl ester diphenyl ester
- Details on test material:
- Batch number: 10264/10/006
Molecular formula: C30H28O10P2
Molecular weight [g/mol]: 610.5
Aggregate state: solid
Colour: white
Purity: 99.7 mol %
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Activated sludge from a municipal sewage plant. Origin: Municipal waste treatment plant Mannheim, Germany
The inoculum was collected on 09 February 2011 from the aeration tank of the plant and areated in the laboratory until use.
Concentration of sludge: 6.0 g/L dry weight. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- ca. 34 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
Test temperature: 22 +/- 2 °C
pH: 7.4 +/- 0.2
pH adjusted: yes
TEST SYSTEM
number of culture flasks/concentration: 2
Test performed in open system: yes
The test bottles were connected to two serial scrubbing bottles (total volume 250 ml) filled with 100 ml sodium hydroxide solution (0.05 mol) for the adsorption of carbon dioxide from biodegradation process.
SAMPLING
sampling frequency: 2/week
CONTROL and BLANK SYSTEM
Inoculum blank: yes
Abiotic sterile control: no
Toxicity control: yes
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 30 - 40
- Sampling time:
- 28 d
- Details on results:
- Duration of the adaptation phase: 11 days
Degradation (CO2 evolution): 9% after 9 days, 21% after 14 days, 30% after 20 days, 33% after 25 days, 35% after 28 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: Not readily biodegradable according to the OECD criteria
- Conclusions:
- Partly/moderately biodegradable
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