Bis(1-oxotetradecanyloxyphenyl)phenylmethane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study with detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Fraunhofer IME

Test material

Test material information

Constituent 1

Reference substance name:

2-{phenyl[2-(tetradecanoyloxy)phenyl]methyl}phenyl tetradecanoate

EC Number:

941-526-9

Molecular formula:

C47H68O4

IUPAC Name:

2-{phenyl[2-(tetradecanoyloxy)phenyl]methyl}phenyl tetradecanoate

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Due to the very low water solubility the Water Accomodated Fraction (WAF) was applied. The required amount of the test item (6.3, 12.6, 24.9, 50.1 and 100.4 mg) was added in 1 l dilution water and was stirred for approx. 48 hrs at room temperature.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Clone V
- Source: German Federal Environment Agency, Institut für Wasser-, Boden- und Lufthygiene
- Age at study initiation: 4-24 h
- Method of breeding: Adult Daphnia, at least 3 weeks old, were separated from the stock population by sieving. Batches of 30 - 50 animals were held at room temperature in ca. 1.8 L of dilution water for one week. Algae growing in the log-phase were centrifuged and the pellet was re-suspended in a few mL of medium. Thirty millilitres of this suspension was given to 1 L of medium. The water was changed once per week.
- Feeding during test: no
- Food type: algal suspension (Desmodesmus subspicatus) and LiquizellR (HOBBY)

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Test temperature:

19.8-19.9 °C

pH:

8.2 – 8.3

Dissolved oxygen:

8.3 – 8.9 mg/L

Nominal and measured concentrations:

100.4, 50.1, 24.9, 12.6, 6.3 mg/L (nominal concentrations)

Details on test conditions:

TEST SYSTEM
- Test vessel: 60 mL glass beakers
- Aeration: not during test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Purified drinking water (filtration with activated charcoal, passage through a lime-stone column and aeration)

OTHER TEST CONDITIONS
- Photoperiod: light/dark cycle 16/8 hours
- Light intensity: 499 – 509 lux

EFFECT PARAMETERS MEASURED :
Immobilisation and sublethal effects, determined after 24 and 48 h

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Results with reference substance (positive control):

Control: Dilution water only

Any other information on results incl. tables

No statistically significantly increased mortality up to a nominal loading of 100.4 mg test item per liter.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

AVG-42 is not acute toxic to Daphnia magna under the chosen test conditions. NOEC and EC50 are >= 100.4 mg/l based on nominal loading.