Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
17.09.2007 - 25.10.2007
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
Formula: C12H14ClN5O4
Appearance: White to pale yellow solid
Expiry date: 31 December 2007

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
15 mg/L
Based on:
other: Nominal carbon concentration
Reference substance
Reference substance:
other: sodium benzoate

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
26.7
Sampling time:
28 d
Details on results:
Points of degradation plot (test substance):
4.1 % degradation after 4 d
12.5 % degradation after 10 d
14.3 % degradation after 15 d
26.7 % degradation after 28 d

BOD5 / COD results

Results with reference substance:
Points of degradation plot (reference substance):
41.8 % degradation after 4 d
73.2 % degradation after 10 d
78.7 % degradation after 15 d
95.2 % degradation after 28 d

Any other information on results incl. tables

The reference substance sodium benzoate was degraded by 73.2 % within 10 days.

Degradation in the toxicity control, which contained sodium benzoate and the test substance, was not indicative of an inhibition of the microbial activity by the test substance.

The biodegradation of "Fam-stage-1" with a nominal concentration of 15 mg carbon/L reached 26.7 % after 28 days of incubation at 22.7 ± 0.3 °C.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not inherently biodegradable
Conclusions:
According to the results of this study, "Fam-stage-1" cannot be classified as readily biodegradable.