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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

The 72 hour ErC50 and the EbC50 of cyclohexylidenebis[tert-butyl] peroxide in a static test system are > 10.0 mg/L expressed as initial loading rate (EL50), corresponding to approximately 0.5 mg/L expressed as actual concentration (the assumed limit of solubility), for Pseudokirchneriella subcapitata.

Key value for chemical safety assessment

EC50 for freshwater algae:
0.5 mg/L
EC10 or NOEC for freshwater algae:
0.5 mg/L

Additional information

The acute toxicity of cyclohexylidenebis[tert-butyl] peroxide was evaluated in the algal strain Pseudokirchneriella subcapitata using a 72 hour static test according to OECD guideline no. 201 and EU method C.3. As the equilibrium between the test item and the aqueous phase depends on the ratio of the test item to liquid a WSF (water-soluble fraction) was prepared for each test solution separately. Test solutions were stirred between 20 and 24 hours and WSFs were obtained by filtration through filters of porosity 0.45 µm.

The 72 hour ErC50 and the EbC50 of cyclohexylidenebis[tert-butyl] peroxide in a static test system are > 10.0 mg/L expressed as initial loading rate (EL50), corresponding to approximately 0.5 mg/L expressed as acutual concentration (the assumed limit of solubility), for Pseudokirchneriella subcapitata. The NOEC at 72 hours is >= 10.0 mg/L expressed as initial loading rate (NOEL), corresponding to approximately 0.5 mg/L expressed as actual concentration, based on the specific growth rat and biomass data.