6-[[(4-methylphenyl)sulphonyl]amino]hexanoic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012-06-26 to 2012-10-10

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: own rearing, original source Water Research Institute, Bratislava, Slovak Republic
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

151 mg/L (CaCO3)

Test temperature:

21.2 - 22.0 °C

pH:

8.12 - 8.23

Dissolved oxygen:

5.49 - 6.65 mg/L

Nominal and measured concentrations:

definite test: 432, 648, 972, 1458, 2187 mg/L (nominal concentration, initial)
measured concentrations: -15.02 to +13.78% of nominal

Details on test conditions:

TEST SYSTEM
- Glass test vessel: 10 mL
- Type: closed
- Materia: glass or other chemically inert material
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL/animal

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water, ISO 6341 test water
- Chlorine: < 10 µg/L
- Intervals of water quality measurement: oxygen and pH measured at beginning and end of test

OTHER TEST CONDITIONS
- Adjustment of pH: yes, 1 N NaOH to pH 8.2
- Photoperiod: 16 h light / 8 h dark

EFFECT PARAMETERS MEASURED:
immobilisation recorded at 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5
- Range finding study: yes

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

1 618 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1 173 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- 48 h-EC50: 0.68 mg/L (mean of 3 determinations)

Immobilisation data:

Nominal concentration [mg(L]	Immobilisation 24 h [%]	Immobilisation 48 h [%]
control	0	0
432	5	5
648	10	15
972	20	30
1458	35	50
2187	75	95

 

Validity criteria fulfilled:

yes

Executive summary:

The acute toxicity of ASC Plus to Daphnia magna was tested according to OECD guideline 202. The 48h-EC50 was determined to be 1173 mg/L.

Description of key information

The acute toxicity of ASC Plus to Daphnia magna was tested according to OECD guideline 202.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 173 mg/L