6-[[(4-methylphenyl)sulphonyl]amino]hexanoic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Source: fish farmer Branislav Settey, SKLOCHEM-AGROEKOLAB, Zvolen
- Length at study initiation: 2.0 +- 1.0 cm
- Feeding during test: none

ACCLIMATION
- Acclimation period: held in laboratory 12 days before test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

151 mg/L (CaCO3)

Test temperature:

21.1°C - 22.3°C

pH:

8.13 - 8.22

Dissolved oxygen:

63.75 - 82.61% of saturation

Nominal and measured concentrations:

4200.0, 4326.0, 4455.8, 4589.0, 4727.1, 4869.0 mg/L (nominal concentrations, initial)
measured concentrations: max. -10.00% of nominal

Details on test conditions:

TEST SYSTEM
- Type: closed
- Materia: glass or other chemically inert material
- Aeration: not during test
- No. of organisms per concentration: 7
- Biomass loading rate: max. 1.0 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO 6341 test water
- Chlorine:< 10 µg/L
- Intervals of water quality measurement: oxygen and pH measured daily

OTHER TEST CONDITIONS
- Adjustment of pH: yes, 1 N NaOH to pH 8.2
- Photoperiod: 16 h light / 8 h dark:

EFFECT PARAMETERS MEASURED:
mortality recorded after 24, 48, 72 and 96 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.03
- Range finding study: yes

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

4 693 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

4 436 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

- Mortality: 96h-EC50 = 115.40 mg/L (mean of 3 determinations)

Sublethal observations / clinical signs:

Mortality data:

Nominal concentration [mg/L]	Mortality 48 h [%]	Mortality 96 h [%]
4200.0	0	0
4326.0	14	43
4455.8	14	57
4589.5	29	71
4727.1	43	86
4869.0	86	100
Control	0	0

 

Validity criteria fulfilled:

yes

Executive summary:

The acute toxicity of ASC Plus to the fish Poecilia reticulata was tested according to OECD guideline 203. The test resulted in a 96h-LC₅₀ of 4436 mg/L.

Description of key information

The acute toxicity of ASC Plus to the fish Poecilia reticulata was tested according to OECD guideline 203.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

4 436 mg/L