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EC number: 907-284-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- please see below "principles of method if other than guideline"
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Principles of method if other than guideline:
- Deviations from the Study Plan
The following deviations were observed:
• The temperature in the experiment was in the range 21.5 – 24.7 °C, and therefore not in the desired range (21.0 - 24.0 °C). As all validity criteria were met and expo-nential growth was observed in the blank control this was stated as uncritical.
• The temperature during pre-culture incubation was in the range 19.7 - 22.5 °C, and therefore not in the desired range (21.0 - 24.0 °C). As all validity criteria were met and exponential growth was observed in the blank control this was stated as uncriti-cal. The deviations were assessed and signed by the study director on 15. May 2019. 13.2
Deviations from the Guideline
The following deviations were observed:
• The stock solutions to be autoclaved were autoclaved for 20 minutes instead of the required 15 minutes. This can be considered uncritical, as the goal of sterility is achieved.
• The temperature in the experiment was in the range 21.5 – 24.7 °C, and therefore not in the desired range (21.0 - 24.0 °C). As all validity criteria were met and expo-nential growth was observed in the blank control this was stated as uncritical.
• The temperature during pre-culture incubation was in the range 19.7 – 22.5 °C, and therefore not in the desired range (21.0 - 24.0 °C). As all validity criteria were met and exponential growth was observed in the blank control this was stated as uncriti-cal - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Methyl (9Z,12Z,15Z)-9,12,15-octadecatrienoate
- EC Number:
- 206-102-3
- EC Name:
- Methyl (9Z,12Z,15Z)-9,12,15-octadecatrienoate
- Cas Number:
- 301-00-8
- Molecular formula:
- C19H32O2
- IUPAC Name:
- methyl octadeca-9,12,15-trienoate
- Reference substance name:
- Methyl linoleate
- EC Number:
- 203-993-0
- EC Name:
- Methyl linoleate
- Cas Number:
- 112-63-0
- Molecular formula:
- C19H34O2
- IUPAC Name:
- methyl octadeca-9,12-dienoate
- Reference substance name:
- Methyl oleate
- EC Number:
- 203-992-5
- EC Name:
- Methyl oleate
- Cas Number:
- 112-62-9
- Molecular formula:
- C19H36O2
- IUPAC Name:
- methyl (Z)-octadec-9-enoate
- Reference substance name:
- Methyl palmitate
- EC Number:
- 203-966-3
- EC Name:
- Methyl palmitate
- Cas Number:
- 112-39-0
- Molecular formula:
- C17H34O2
- IUPAC Name:
- methyl palmitate
- Test material form:
- liquid
Constituent 1
Constituent 2
Constituent 3
impurity 1
- Specific details on test material used for the study:
- Batch no. 18010144
Appearance colourless to pale yellow liquid
Composition METHYL OLEATE [CAS 112-62-9] 17 - 23 %;
METHYL LINOLEATE [CAS 112-63-0] 12 - 20 %;
METHYL LINOLENATE [CAS 301-00-8] 47-53 %;
OTHER METHYL ESTERS 4 - 16 %
CAS No. not applicable
EC-No. 907-284-3
Purity GC - 50.25 % linolenate methyle
Homogeneity homogeneous
Production date 15. Feb. 2018
Expiry date 15. Feb. 2020
Storage Room Temperature (20 ± 5°C)
Specific gravity 0.8872
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- 6 g NaCl was added to 100 mL test solution resp. blank control solution; then, the clear solution was extracted two times with the solvent dichloromethane (2 x 4 mL), the organic phase was collected into a flask after drying with Na2SO4, the solvent dichloromethane was evaporated at 40 °C and the residue was dissolved in 2 mL of 10 mg/L ISTD (internal standard) stock solution and the solution was measured via GC/MSD.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Unicellular freshwater green alga.
Genus Desmodesmus
Species subspicatus
SAG Strain Number 86.81
Taxonomic position Chlorophyta - Chlorophyceae
Origin and Culture
The culture of Desmodesmus subspicatus was obtained in January 2016 by MBM Sci-encebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen). The algae are kept as stock culture on solid agar at 2 - 8 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21.5 – 24.7 °C
- pH:
- 7.7
- Nominal and measured concentrations:
- nominal concentration: 100mg/L
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)
Results and discussion
Effect concentrations
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
The toxicity to aquatic algae and cyanobacteria was performed according to the OECD 101 guideline.
The test substance has the following EC50 72h: >100 mg/L.
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