1-(9,9-Dibutyl-9H-fluoren-2-yl)-2-methyl-2-morpholin-4-yl-propan-1-one

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1-(9,9-dibutyl-9H-fluoren-2-yl)-2-methyl-2-(morpholin-4-yl)propan-1-one

EC Number:

819-558-9

Cas Number:

2020359-04-8

Molecular formula:

C29H39NO2

IUPAC Name:

1-(9,9-dibutyl-9H-fluoren-2-yl)-2-methyl-2-(morpholin-4-yl)propan-1-one

Test material form:

solid: particulate/powder

Details on test material:

a off-white powdery solid, without irritating odor

Test solutions

Vehicle:

yes

Details on test solutions:

This study was conducted semi-static system. Preparation method of test solution was calculated from the purity of 99.84 %.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Fish(Zebrafish, Danio rerio) has been used in freshwater toxicity studies, and a large comparable historical database exists; therefore, it was selected for this study.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

Fish in the treatment groups and control group were observed for general symptoms at 3, 6, 24, 48, 72 and 96 hrs after exposure and the mortality was recorded.

Test conditions

Test temperature:

21.7 ~ 22.4 °C

pH:

7.67 ~ 7.93

Dissolved oxygen:

8.37 ~ 8.63 mg/L(94.5 ~ 97.4 %)

Nominal and measured concentrations:

10.0 and 100.0 mg/L.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.006 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 0.006 mg/L

Details on results:

Based on the range finding test, limit test was performed in control, 100.0 mg/L nominal concentration. As a result of the stability test, concentration of the test substance(Purity: 99.84 %) in the test solution was carried out 100.0 mg/L. The measured concentrations of the test substance at the 100.0 mg/L were 0.00631 mg/L at 0 hr, 0.00653 mg/L at 48 hrs, 0.00151 mg/L at 96 hrs, based on 0 hr the test substance was not stable at 96 hrs to 23.9 %, so limit test was conducted with semi-static system. As a result of the limit test, The geometric mean of measured concentration of the test substance at the 100.0 mg/L was 0.00604 mg/L for 96 hrs. These result was 0.00604 % about nominal concentration. Therefore, all test results were
determined based on the geometric mean measured concentration.
At 96 hrs after exposure, the total mean size and weight of negative control fish were 2.8 ± 0.1 cm and 0.153 ± 0.030 g(mean±SD), respectively. treatment group fish were 2.8 ± 0.1 cm and 0.155 ± 0.029 g(mean±SD), respectively.
During the exposure period, the water temperature, dissolved oxygen concentration and pH value of test solution were in the ranges of 22.1 ~ 22.8 °C, 6.80 ~ 8.76 mg/L(converted to air saturation value: 77.4 ~ 100.2 %) and 7.37 ~ 8.07, respectively. These values were within the permissible ranges of this study.
LC50 was calculated based on the geometric means measured concentration.

Applicant's summary and conclusion

Conclusions:

During an exposure period, The LC50 was determined to be > 0.00604 mg/L at 48, 96 hrs after exposure. The highest concentration causing no mortality was determined to be ≥ 0.00604 mg/L at 48 and 96 hrs after exposure, respectively. The lowest concentration causing 100 % mortality was determined to be > 0.00604 mg/L at 48 and 96 hrs after exposure, respectively.