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Long-term toxicity to fish

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Reference
Endpoint:
adult fish: sub(lethal) effects
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
GLP compliance:
no
Specific details on test material used for the study:
no details given
Analytical monitoring:
yes
Details on sampling:
- Concentrations: five concentrations, geometrically increasing with a factor of 1.8
Vehicle:
yes
Remarks:
acetone
Details on test solutions:
no details given
Test organisms (species):
Poecilia reticulata
Details on test organisms:
TEST ORGANISM
- Common name: guppy
- Source: laboratory-reared
- Age at study initiation (mean and range, SD): 2-3 months
- Feeding during test : yes
- Food type: commercial food (TetraPhyll, Tetra Werke, Germany)
- Frequency: daily

ACCLIMATION
- Acclimation period: at least 12 days
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
14 d
Test temperature:
21 - 23°C
pH:
6.5 -7.5
Dissolved oxygen:
Oxygen content was sometimes found to be very low, approx. 3 mg/L, 24 h after preparation of the solutions
Salinity:
not specified
Conductivity:
not specified
Nominal and measured concentrations:
not specified
Details on test conditions:
TEST SYSTEM
- Test vessel: glass vessels
- Size of vessel: 1.5 L
- Type (delete if not applicable): closed with glass lids
- Material, size, headspace, fill volume: 1.4 L
- Renewal rate of test solution (frequency/flow rate): daily renewal
- No. of vessels per concentration (replicates): 10 fish per concentration
- Control: control fish were exposed to 72 µl/L acetone,

TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: 8 times daily (4 times before and 4 times after water renewal)

OTHER TEST CONDITIONS
- Photoperiod: 12h/12h (light/dark)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : LC50

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
Reference substance (positive control):
no
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
97.7 µmol/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Remarks:
The concentrations used in the calculations were based on the added amount of material. The resulting LC50 was then corrected for loss of the compound through evaporation etc. by multiplication with its recovery factor.
Basis for effect:
mortality
Key result
Duration:
14 d
Dose descriptor:
LC50
Remarks:
calculated based on the MW of 100.16 g/mol
Effect conc.:
9.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
The concentrations used in the calculations were based on the added amount of material. The resulting LC50 was then corrected for loss of the compound through evaporation etc. by multiplication with its recovery factor.
Basis for effect:
mortality
Details on results:
no details given
Results with reference substance (positive control):
not specified
Reported statistics and error estimates:
LC50 data were calculated by logit transformation.
Validity criteria fulfilled:
not specified
Conclusions:
An LC50 (14 d) of 9.8 mg/L was determined in P. reticulata under semi-static conditions.
Executive summary:

A long-term toxicity (14 d) assay on the guppy (Poecila reticulata) was conducted with the test item hexanal simliar to OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study). 5 analytically verified concentrations were tested on 10 fish per concentration under semi-static conditions. The LC50 after 14 d was determined by logit transformation. The study is well documented and meets generally accepted scientific principle and is therefore sufficient for the assessment. An LC50 (14 d) value of 97.7 µmol/L, which corresponds to 9.8 mg/L was derived.

Description of key information

A long-term toxicity (14 d) assay on the guppy (Poecila reticulata) was conducted with the test item hexanal simliar to OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study). 5 analytically verified concentrations were tested on 10 fish per concentration under semi-static conditions. The LC50 after 14 d was determined by logit transformation. The study is well documented and meets generally accepted scientific principle and is therefore sufficient for the assessment. An LC50 (14 d) value of 97.7 µmol/L, which corresponds to 9.8 mg/L was derived.

Key value for chemical safety assessment

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