Morpholine, 4-[(triethoxysilyl)methyl]-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007-06-28 to 2007-11-20

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 92/69/EWG, C.2; OECD 202 (2004)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

480-370-1

EC Name:

-

Cas Number:

21743-27-1

Molecular formula:

Hill formula: C11H25NO4Si CAS formula: C11H25NO4Si

IUPAC Name:

4-[(triethoxysilyl)methyl]morpholine

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

All concentrations at 0 and 48 h

Test solutions

Vehicle:

no

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: No vehicle used.
The highest test concentration of nominal 144 mg/l was prepared by dissolving 316.7 mg of test item into 2200 ml of culture medium and by intense stirring for 24 h.
Since the test item undergoes rapid hydrolysis and as there was no specific analytical method available, analyses was performed by determination of the DOC (Dissolved organic carbon) content as sum parameter in the test media.
At the start of the test just before introduction of the Daphnia 97% of the nominal test concentration were found. After 48 hours test duration 90 % of the nominal values were determined. Thus during the test period of 48 hours the Daphnia were exposed to a mean of 93% of nominal.
Only at 0h in the test concentrations 100-144 mg/L slight prcipitation was observed after preaparation of the test item

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus, clone 5
- Source: ECT Oekotoxikologie GmbH, Flörsheim, Germany
- Age at study initiation (mean and range, SD): 2.5 to 18 hours
- Feeding during test: no


ACCLIMATION
not necessary

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

no

Test conditions

Hardness:

250 mg CaCO3/L

Test temperature:

19-21 °C

pH:

7.7-8.4

Dissolved oxygen:

7.9-9.1 mg/L

Salinity:

0.9 mmol/L

Nominal and measured concentrations:

0, 69, 83, 100, 120, 144 mg/L nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers of 100 mL containing 80 mL of culture medium
- Type (delete if not applicable): closed with a lid to reduce loss of water
- Renewal rate of test solution (frequency/flow rate):-
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
see above


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h
- Light intensity: 350 - 570 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The immobility of the Daphnia was determined by visual controls after 24 and 48 h. Those animals not able to swim within 15 seconds after gentle agitation of the test beaker were considered to be immobile.


TEST CONCENTRATIONS
- Spacing factor for test concentrations: < 2.2
- Range finding study
- Test concentrations: <100 mg/L nominal

Reference substance (positive control):

yes

Remarks:

potassium dichromate (twice a year)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

120 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 144 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 144 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: no
- Other biological observations: no
- Mortality of control: 0
- Other adverse effects control: no
- Abnormal responses: no
- Effect concentrations exceeding solubility of substance in test medium: no
At the test concentration of 144 mg/L one daphnia was immobile, which was not assessed as significant by statistical evaluation

Results with reference substance (positive control):

no details

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

N-(Morpholinomethyl)triethoxysilane VP was not toxic to the water flea Daphnia magna under the conditions of the test.

Executive summary:

To assess the acute toxicity of N-(Morpholinomethyl)triethoxysilane to aquatic invertebrates (Daphnia magna) a test according to OECD 202 (EU Guideline 92/92/EEC C.2) was performed. In the control and the concentrations 69, 83, 100 and 120 mg/L (nominal) no immobile daphnias were observed. At the test concentration of 144 mg/L one daphnia was immobile, which was not assessed as significant by statistical evaluation. The measured concentrations were at least 93% of the nominal. Based on this N-(Morpholinomethyl)triethoxysilane is not considerd to be toxic to Daphnia magna.