Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: screening test, other
Remarks:
anaerobic degradation
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
no data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Microbial degradation of nitrogen, oxygen and sulfur heterocyclic compounds under anaerobic conditions: studies with aquifer samples
Author:
Kuhn, E. & J. Suflita.
Year:
1989
Bibliographic source:
U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/353
Report Date:
1989

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The anaerobic biodegradation under sulfate-reducing and methanogenic-conditions were assessed at lab scale using aquifer samples
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
anaerobic
Inoculum or test system:
natural sediment: freshwater
Details on inoculum:
- Aquifer solids and site water sampled from 2 well-characterized sites adjacent to the Normann municipal landfill.
- Methanogenic site: 1.35m below surface, temperature 18.3°C, pH of 6.8, TOC = 288 mg/L, sulfate concentration < 0.1 mM
- Sulfate-reducing site: 1.15m below surface, temperature 15.6°C, pH of 7.2; TOC = 14.4 mg/L, sulfate concentration of 2.1 mM
Duration of test (contact time):
8 mo
Initial test substance concentration
Initial conc.:
0.2 mmol/L
Based on:
test mat.
Parameter followed for biodegradation estimationopen allclose all
Parameter followed for biodegradation estimation:
test mat. analysis
Remarks:
substrate remaining
Parameter followed for biodegradation estimation:
CH4 evolution
Remarks:
CH4 formation

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (test mat. analysis)
Value:
0
Sampling time:
1 mo
Remarks on result:
other: sulfate-reducing aquifer slurries
Parameter:
% degradation (test mat. analysis)
Value:
71
Sampling time:
3 mo
Remarks on result:
other: sulfate-reducing aquifer slurries
Parameter:
% degradation (test mat. analysis)
Value:
66
Sampling time:
8 mo
Remarks on result:
other: sulfate-reducing aquifer slurries
Parameter:
% degradation (test mat. analysis)
Value:
0
Sampling time:
1 mo
Remarks on result:
other: methanoganic aquifer slurries
Parameter:
% degradation (test mat. analysis)
Value:
0
Sampling time:
3 mo
Remarks on result:
other: methanoganic aquifer slurries
Parameter:
% degradation (test mat. analysis)
Value:
0
Sampling time:
8 mo
Remarks on result:
other: methanoganic aquifer slurries

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Interpretation of results:
other:
Remarks:
anaerobic biodegradation is only observed in the sulfate-reducing aquifer slurries. No anaerobic biodegradation is observed in methanogenic aquifer slurries
Conclusions:
The substance 2-methylfuran showed anaerobic biodegradation under sulfate-reducing conditions: roughly 70 % degraded within 3 months of exposure. Under methanoganic conditions, no anaerobic biodegradation was observed.
Executive summary:

The potential anaerobic biodegradation of 2 -methylfuran was studied under sulfate-reducing or methanogenic conditions in aquifer slurries. The disappearance of the substance was assessed over 8 month-period.

The substance 2-methylfuran showed anaerobic biodegradation under sulfate-reducing conditions: roughly 70 % degraded within 3 months of exposure. Under methanoganic conditions, no anaerobic biodegradation was observed.