Registration Dossier
Registration Dossier
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EC number: 246-857-6 | CAS number: 25333-42-0
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- (R)-quinuclidin-3-ol
- EC Number:
- 246-857-6
- EC Name:
- (R)-quinuclidin-3-ol
- Cas Number:
- 25333-42-0
- Molecular formula:
- C7H13NO
- IUPAC Name:
- quinuclidin-3-ol
- Test material form:
- solid: crystalline
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Test solution were prepared by adding a 100 mg/l stock solution (preapared by direct addition of the test material to water, with stirring) to diution water in appropriate quantities to obtain the desired test concentrations.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: Reading University
- Age of parental stock (mean and range, SD): Less 24h at start of test
- Feeding during test: no
- Food type: prior to testing, a suspension of Chlorella vulgaris
- Amount: 1mg organic carbon per litre of culture water
- Frequency: each working day
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 231 mg/L CaCO3
- Test temperature:
- 20 +/- 1°C
- pH:
- 7.2 +/- 0.2
- Dissolved oxygen:
- minimum 60%
- Nominal and measured concentrations:
- Nominal: 0, 10, 18, 32, 56, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL crystalline dishes
- Type: open - transparent perspex sheet was placed on top of dishes
- Material, size, headspace, fill volume: glass, 25mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h light, 8h dark
RANGE-FINDING STUDY
- Test concentrations: 0, 0.32, 1.0, 3.2, 10, 32, 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Two main studies conducted as the results of the first main study (no immobility in any test concentration was seen) was not expected based on the results of the preliminary study. Both main studies gave the same results EC50 >100 mg/L.
- Results with reference substance (positive control):
- - Results with reference substance valid?
yes
- Relevant effect levels: 48h EC50 0.8 mg/L
Any other information on results incl. tables
Table 1: Preliminary range finding study - cumulative % immobilisation
| Concentration (mg/L) | No. immobilised | % immobilisation | ||
| 24 hours | 48 hours | 24 hours | 48 hours | |
| 0 | 0 | 1 | 0 | 20 |
| 0.32 | 0 | 1 | 0 | 20 |
| 1.0 | 0 | 1 | 0 | 20 |
| 3.2 | 1 | 1 | 20 | 20 |
| 10 | 0 | 1 | 0 | 20 |
| 32 | 2 | 3 | 40 | 60 |
| 100 | 2 | 5 | 40 | 100 |
Table 2: First main study - cumulative % immobilisation
| Concentration (mg/L) | No. immobilised | % immobilisation | ||
| 24 hours | 48 hours | 24 hours | 48 hours | |
| 0 | 0 | 0 | 0 | 0 |
| 10 | 0 | 0 | 0 | 0 |
| 18 | 0 | 0 | 0 | 0 |
| 32 | 0 | 0 | 0 | 0 |
| 56 | 1 | 1 | 5 | 5 |
| 100 | 0 | 0 | 0 | 0 |
Table 3 - Second main study - culumative % immobilisation
| Concentration (mg/L) | No. immobilised | % immobilisation | ||
| 24 hours | 48 hours | 24 hours | 48 hours | |
| 0 | 0 | 0 | 0 | 0 |
| 10 | 0 | 0 | 0 | 0 |
| 18 | 0 | 0 | 0 | 0 |
| 32 | 0 | 0 | 0 | 0 |
| 56 | 0 | 0 | 0 | 0 |
| 100 | 0 | 0 | 0 | 0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48h EC50 value for the test substance was >100mg/L.
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