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EC number: 218-489-6 | CAS number: 2162-98-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Pyridine-2,6-dicarboxylic acid
- EC Number:
- 207-894-3
- EC Name:
- Pyridine-2,6-dicarboxylic acid
- Cas Number:
- 499-83-2
- Molecular formula:
- C7H5NO4
- IUPAC Name:
- 2,6-Pyridinedicarboxylic Acid Pyridin-2,6-dicarbonsäure Pyridine-2,6-dicarboxylic Acid
- Test material form:
- solid: crystalline
Constituent 1
Method
- Target gene:
- TA98:
his D 3052; rfa-; uvrB-; R-factor: frame shift mutations
TA 100:
his G 46; rfa-; uvrB-; R-factor: base-pair substitutions
TA 1535:
his G 46; rfa-; uvrB-: base-pair substitutions
TA 1537:
his C 3076; rfa-; uvrB-: frame shift mutations
TA 102:
his G 428 (pAQ1); rfa-; R-factor: base-pair substitutions
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 Homogenate
- Test concentrations with justification for top dose:
- The test item concentrations to be applied in the main experiments were chosen according to the results of the pre-experiment (see chapter 12.1 Pre-Experiment). 5000 µg/plate was selected as the maximum concentration. The concentration range
covered two logarithmic decades. Two independent experiments were performed with the following concentrations: Experiment I: 3.16, 10.0, 31.6, 100,316, 1000,2500 and 5000 µg/plate (all tester strains except TA 100 without metabolic activation)
0.100,0.316, 1.00,3.16, 10.0,31.6, 100,316, 1000and 2500 µg/plate (only TA 100 without metabolic activation) Experiment II: 8, 25, 70, 200, 600, 1000, 1800 and 5000 llg/plate (all tester strains except TA 98 and TA 100 without metabolic activation) 0.25, 0.8, 2.5, 8, 25, 70, 200, 600, 1000 and 1800 µg/plate (only TA 98 and TA 100 without metabolic activation). As the results of the pre-experiment TA 98 with and without metabolic activation and TA 100 with metabolic activation were in accordance with the criteria described above, these were reported as a part of the main experiment I. - Vehicle / solvent:
- The test item was dissolved in EtOH and diluted prior to treatment. The solvent was compatible with the survival ofthe bacteria and the S9 activity.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- methylmethanesulfonate
- other: 4-nitro-o-pheny1ene-diamine; 2-aminoanthracene
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: In tester strain TA 1535 toxic effects ofthe test item were noted at concentrations of 70 µg/plate and higher (without metabolic activation) and at concentrations of 200 µg/plate and higher (with metabolic activation).
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: TA 153 7 toxic effects of the test item were observed at concentrations of 70 µg/plate and higher (without metabolic activation) and at concentrations of 600 µg/plate and higher (with metabolic activation).
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: at concentrations of 31.6 µg/plate and higher (without metabolic activation).
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: In tester strain TA 100 toxic effects of the test item were noted at concentrations of 10 µg/plate and higher (without metabolic activation) and at concentrations of 100 µg/plate (with metabolic activation) and higher.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
The test item 1, 1 0-Dichlorodecane was investigated for its potential to induce gene mutations according to the plate incorporation test ( experiment I and II) using Salmonella typhimurium strains TA 98, TA 100, TA 1535, TA 1537 and TA 102. In two independent experiments several concentrations of the test item were used. Bach assay was conducted with and without metabolic activation. The concentrations, including the controls, were tested in triplicate. The following concentrations of the test item were prepared and used in the experiments: Experiment 1: 3.16, 10.0, 31.6, 100,316, 1000,2500 and 5000 µg/plate (all tester strains except TA 100 without metabolic activation) 0.100, 0.316, 1.00, 3.16, 10.0, 31.6, 100,316, 1000 and 2500 µg/plate (only TA 100 without metabolic activation) Experiment II: 8, 25, 70, 200, 600, 1000, 1800 and 5000 µg/plate (all tested strains except TA 98 and TA 100 without metabolic activation) 0.25, 0.8, 2.5, 8, 25, 70, 200, 600, 1000 and 1800 µg/plate (only TA 98 and TA 100 without metabolic activation). No precipitation of the test item was observed in any tester strain used in experiment I and II (with and without metabolic activation). Toxic effects of the test item were noted in four of five tester strains evaluated in experiment I and II. In experiment I toxic effects of the test item were observed in tester strain TA 98 at concentrations of 31.6 µg/plate and higher (without metabolic activation). In tested strain TA 100 toxic effects of the test item were noted at concentrations of 10 µg/plate and higher (without metabolic activation) and at concentrations of 100 µg/plate (with metabolic activation) and higher. In tester strain TA 1535 toxic effects of the test item were noted at concentrations of 2500 µg/plate and higher (without metabolic activation) and at concentrations of 316 µg/plate (with metabolic activation). In tester strain TA 153 7 toxic effects of the test item were observed at concentrations of 316 µg/plate and higher (with and without metabolic activation). In experiment II toxic effects of the test item were noted in tester strain TA 98 at concentrations of 70 µg/plate and higher (without metabolic activation). In tester strain TA 100 toxic effects of the test item were noted at concentrations of 25 µg/plate and higher (without metabolic activation) and at concentrations of 200 µg/plate and higher (with metabolic activation). In tester strain TA 1535 toxic effects ofthe test item were noted at concentrations of 70 µg/plate and higher (without metabolic activation) and at concentrations of 200 µg/plate and higher (with metabolic activation). In tester strain TA 1537 toxic effects of the test item were observed at concentrations of 70 µg/plate and higher (without metabolic activation) and at concentrations of 600 µg/plate and higher (with metabolic activation). No biologically relevant increases in revertant colony numbers of any of the five tester strains were observed following treatment with 1,10-Dichlorodecane at any concentration level, neither in the presence nor absence of metabolic activation in experiment I and II. The reference mutagens induced a distinct increase of revetiant colonies indicating the validity ofthe experiments.
Applicant's summary and conclusion
- Conclusions:
- In conclusion, it can be stated that during the described mutagenicity test and under the
experimental conditions reported, 1,1 0-Dichlorodecane did not cause gene mutations by
base pair changes or frameshifts in the genome of the tester strains used.
Therefore, 1,1 0-Dichlorodecane is considered to be non-mutagenic in this bacterial
reverse mutation assay - Executive summary:
Summary Results
In order to investigate the potential of 1,10-Dichlorodecane for its ability to induce gene
mutations the plate incorporation test ( experiment I and II) was performed with the
Salmonella typhimurium strains TA 98, TA 100, TA 1535, TA 1537 and TA 102.
In two independent experiments several concentrations of the test item were used. Bach
assay was conducted with and without metabolic activation. The concentrations,
including the controls, were tested in triplicate. The following concentrations of the test
item were prepared and used in the experiments:
Experiment 1:
3.16, 10.0, 31.6, 100, 316, 1000,2500 and 5000 f.tg/plate
(all tester strains except TA 100 without metabolic activation)
0.100, 0.316, 1.00, 3.16, 10.0, 31.6, 100, 316, 1000 and 2500 f.tg/plate
( only TA 100 without metabolic activation)
Experiment II:
8, 25, 70, 200, 600, 1000, 1800 and 5000 f.tg/plate
(all tester strains except TA 98 and TA 100 without metabolic activation)
0.25, 0.8, 2.5, 8, 25, 70,200,600, 1000 and 1800 f.tg/plate
( only TA 98 and TA 100 without metabolic activation)
No precipitation of the test item was observed in all tester strains used in experiment I
and II (with and without metabolic activation).
Toxic effects of the test item were noted in four tester strains used in experiment I
and II:
• In experiment I toxic effects of the test item were observed at concentrations of
10.0 f.tg/plate and higher (without metabolic activation) and at concentrations of
100 f.tg/plate and higher (with metabolic activation), depending on the particular
tester strain.
• In experiment II toxic effects of the test item were noted at concentrations of
25 f.tg/plate and higher (without metabolic activation) and at concentrations of
200 f.tg/plate and higher (with metabolic activation), depending on the particular
tester strain.
No biologically relevant increases in revertant colony numbers of any of the five tester
strains were observed following treatment with 1,10-Dichlorodecane at any
concentration level, neither in the presence nor absence of metabolic activation in
experiment I and II.
The reference mutagens induced a distinct increase of revertant colonies indicating the
validity of the experiments.
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