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EC number: 224-662-7 | CAS number: 4441-12-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Dec 18, 2001 - Apr 02, 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Principles of method if other than guideline:
- none
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Trimorpholinophosphine oxide
- EC Number:
- 224-662-7
- EC Name:
- Trimorpholinophosphine oxide
- Cas Number:
- 4441-12-7
- Molecular formula:
- C12H24N3O4P
- IUPAC Name:
- trimorpholinophosphine oxide
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1000 mg lest item were dissolved in 200 mL deionized water. Thus, a concentration of 5.0 g/L was obtained.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Species: activated sludge, micro organisms from a domestic waste water treatment plant.
Origin: supplied by the sewage plant Groß Zimmern, Germany
The sludge was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and centrifuged again. The latter procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge (g) to its dry weight (g) determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 3 g dry material/L were made up with tap water. The pH of the activated sludge was determined to be 7.8. The activated sludge was used directly after adjustment without storage.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- 250 mg/L
- Test temperature:
- 19 - 20 °C
- pH:
- saturated (7-8 mg/L)
- Dissolved oxygen:
- >2.5 mg O2/L
- Nominal and measured concentrations:
- 0, 10, 32, 100, 320, 1000 mg/L nominal (test material)
0, 3.2, 10, 32 mg/L nominal (reference 3,5-Dichlorophenol) - Details on test conditions:
- Measurement of Respiration Rate:
For the measurement of the respiration rate a well-mixed sample of each test medium was poured into a Karlsruher flask after exactly 3 hours incubation time, and was not further aerated. The oxygen concentration was measured with an oxygen electrode, and was recorded for about ten minutes. During measurement, the samples were continuously stirred on a magnetic stirrer.
The oxygen consumption (in mg 02 per L per minute) was determined from the most linear part of the respiration curve.
Measurement of pH, dissolved oxygen and water temperature:
The pH and the oxygen concentrations were determined at the start and at the end of the incubation period in all treatments. The water temperature was measured in one control medium at the start and at the end of the incubation period. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Inhibition of respiration rate:
In comparison to the inoculum controls the respiration rate of the activated sludge was not inhibited (-4.9%) at the lowest concentration of 10 mg/L. At the nominal concentrations of 32 and 320 mg/L, the respiration rate was inhibited by 8.7%. St the highest nominal test concentration of 1000 mg/L an inhibition rate of 16.5% was determined. The test item concentrations exceeding 1000 mg/L were not tested.
3-hour EC20 and 3-hour EC50:
Based on measured inhibition rates, the 3-hour EC20 and EC50 could not be quantified, because up to the highest nominal test concentration (1000 mg/L) less than 20% inhibition was noted after 3 hours of incubation. Nevertheledd, the 3-hour EC20 and EC50 are clearly higher than 1000 mg/L nominal under the experimental conditions chosen. - Results with reference substance (positive control):
- Inhibition of respiration rate:
In comparison to the controls the respiration rate of the activated sludge was moderately inhibited by 34% at the lowest nominal concentration of 3.2 mg/L. At the nominal concentrations of 10 and 32 mg/L, the respiration rate was inhibited by 67% and 88.4%, respectively.
3-hour EC20 and 3-hour EC50:
The 3-hour EC50 of 3,5-Dichlorophenol was calculated to be 6.0 mg/L with 95% confidence limits from 2.0 to 9.0 mg/L.
Any other information on results incl. tables
The influence of the test item on the activity of activated sludge was evaluated by measuring the respiration rate under defined conditions. The respiration rate (oxygen consumption) of an aerobic activated sludge fed with a standard amount of synthetic sewage was measured in the presence of various concentrations (10, 32, 100, 320 and 1000 mg/L) of the test item after an incubation period of 3 hours. This study was conducted according to Good Laboratory Practice (GLP) and the method applied followed the OECD Guideline for Testing of Chemicals, Section 2, No.209: "Activated Sludge, Respiration Inhibition Test", adopted April 04, 1984.
In comparison to the inoculum controls, the respiration rate of the activated sludge was inhibited by up to 16.5% at a tested concentration of nominal 1000 mg/L. Higher concentrations were not tested. Based on measured inhibition rates, the 3 hour EC20 and EC50 values of the test item could not be quantified as the inhibition at the highest nominal concentration of 1000 mg/L was less than 20%. However, the 3 -hour EC20 and EC50 are clearly higher than 1000 mg/L.
The 3 -hour EC50 of the test material was higher than 1000 mg/L under the present test conditions.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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