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EC number: 229-551-7 | CAS number: 6606-59-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11-Nov. to 15 Nov 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study OECD 203, GLP, analytical monitoring.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1,6-hexanediyl bismethacrylate
- EC Number:
- 229-551-7
- EC Name:
- 1,6-hexanediyl bismethacrylate
- Cas Number:
- 6606-59-3
- Molecular formula:
- C14H22O4
- IUPAC Name:
- 6-[(2-methylprop-2-enoyl)oxy]hexyl 2-methylprop-2-enoate
- Details on test material:
- - Name of test material (as cited in study report): 1,6-Hexanedioldimethacrylate
- Substance type: organic
- Physical state at room temperature: liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- Dilution water: purified drinking water
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Brachydanio rerio (Teleostei, Cyprinidae)
- Strain: Brachydanio rerio (Teleostei, Cyprinidae) (HAMILTON-BUCHANAN 1822)
- Source: West Aquarium GmbH, D-37431 Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): 2.6 ± 0.2 cm (min: 2.3 cm; max: 2.9 cm)
- Weight at study initiation (mean and range, SD): 0.13 g and 0.31 g with a mean weight: 0.22 ± 0.06 g
- Method of breeding: Laboratory bred
- Feeding during test: fish were not fed during the test (beginning 24 h prior to initiation)
- Food type: TetraMin® Hauptfutter
- Amount: no data
- Frequency: daily until 24 h before the test
ACCLIMATION
- Acclimation period: no data
- Acclimation conditions (same as test or not): in dilution water of the same quality as used for the test
- Health during acclimation (any mortality observed): no data
Study design
- Test type:
- semi-static
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- varied between 22.0 ± 0.1 °C
- pH:
- varied between 8.03 and 8.78
- Dissolved oxygen:
- oxygen saturation in all test vessels was between 91.6% and 99.5%
- Nominal and measured concentrations:
- 0.0, 1.1, 2.1, 3.7, 6.7 and 12.0 mg/L (nominal concentrations)
- Details on test conditions:
- Dilution water was prepared as follows. Purified drinking water was used as dilution water. The purification included filtration with charcoal, aeration and passage through a lime stone column. The water was aerated to oxygen saturation prior to preparation of the test solutions.
Test concentrations:
The pure test substance according to the chosen test concentration were pipetted in the test vessel and filled up with dilution water.
Test priciples and procedure:
10 fish were used for each test concentration including controls.
Only fish in good health and free from any apparent malformation were used.
15-l glass aquaria were used as test vessels and filled with 10 l of test solution. The test animals were added to the previously prepared test solution; controls were kept in dilution water (blank). Loading: 0.22 ± 0.06 g*LE-1
The aquaria were aerated via a glass capillary throughout the test.
The fish were exposed to various concentrations of the test substance for a period of 96 hours under semi static conditions. The test medium was changed completely every day. Mortalities and visual abnormalities were recorded at 24 h, 48 h, 72 h, and 96 h.
The test temperature was 22.0 ± 0.1 ° C. The pH of the dilution water was 0.03 to 8.78. The oxygen saturation in all test vessels was between 91.6 % and 99.5 %.
The light/dark rhythm was 14h/10h.
The pH, temperature and the oxygen concentrations of the control and of all the test concentrations were measured daily.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL: 3.88 - 5.28 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 3.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 6.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Cumulative Mortality of 1,6 -HDDMA on Brachydanio rerio (Zebra Fish)Total number of fish per concentration: n= m10. Cumulative mortality of fish Nr Test concentration (mg/l) 24h 48h 72h 96h 0/0 blanc 0 0 0 0 1 1.1 0 0 0 0 2 2.1 0 0 0 0 3 3.7 0 0 0 0 4 6.7 4 4 9 10 5 12.0 8 10 10 10
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- -Criteria of OECD 203: Mortality of controls <1
- Conclusions:
- In a valid guideline study (OECD 203, semi-static conditions), the 96 hr LC50 for Brachydanio rerio was 4.5 mg/L . Normal behaviour of the animals was observed during the whole test period.
- Executive summary:
In a 96-h acute toxicity study, Brachydanio rerio were exposed to 1,6 -Hexanedioldimethacrylate
at 0 (control), 1.1, 2.1, 3.7, 6.7 and 12.0 mg/L nominal concentrations under semi-static conditions. The 96-h LC50was 4.5 mg a.i./L (95% confidence limt: 3.88 - 5.28 mg/L) based on mortality effects. The effect concentrations refer to the nominal concentrations. Based on the results of this study, 1,6 -Hexanedioldimethacrylate would be classified as toxic to Brachydanio rerio (Zebra fish) in accordance with the classification system of the EU. EU Category: chronic 2This toxicity study is classified as acceptable and satisfies the guideline requirement for OECD 203, Brachydanio rerio (Zebra fish) toxicity study.
Results Synopsis
Test organism size/age: mean weight: 0.13 g and 0.31 g with a mean weight: 0.22 ± 0.06 g,
length: 2.6 ± 0.2 cm (min: 2.3 cm; max: 2.9 cm)
Test Type: semi-static
LC50: 4.5 mg a.i./L 95% C.I.: 3.88 - 5.28 mg/L mg a.i./L
LC10: 3.71 mg a.i./L}
Endpoint(s) Effected: mortality
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