Fluorobenzene

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Reference 2

Reference Type:

secondary source

Title:

Toxicological Evaluations Vol. 13

Author:

BG Chemie

Year:

1998

Bibliographic source:

Springer-Verlag Berlin Heidelberg, ISBN 3-540-62658-1

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

observation period 16 days

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Fluorobenzene

EC Number:

207-321-7

EC Name:

Fluorobenzene

Cas Number:

462-06-6

Molecular formula:

C6H5F

IUPAC Name:

fluorobenzene

Details on test material:

- Name of test material (as cited in study report): Fluorobenzene
- Analytical purity: 99.7%
- Batch No. 01420
- Physical state: colourless liquid
- Storage condition of test material: in the refrigerator at 4°C

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Interfauna UK Limited, Wyton, Huntingdon
- Age at study initiation: young adult
- Housing: single housing in aluminium cages with grid floors beneath which were peat moss filled trays
- Diet: Standard Rabbit Diet (Special Diets Services, Witham, Essex) ad libitum
- Water: Tap water ad libitum
- Acclimation period: 12 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18-20
- Humidity (%): 52 (mean)
- Photoperiod (hrs dark / hrs light): 12 / 12


IN-LIFE DATES: From: To:

Test system

Type of coverage:

semiocclusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated skin of same animals

Amount / concentration applied:

TEST MATERIAL
- Amount applied: 0.5 ml
- Concentration: undiluted

Duration of treatment / exposure:

4 hours

Observation period:

16 days; skin reactions were assessed 1 h, 24 h, 48 h, 72 h, 7 d and 16 d after patch removal

Number of animals:

3 males

Details on study design:

TEST SITE
- Area of exposure: dorsal area of the trunk
- Type of wrap if used: 2.5 cm x 2.5 cm patch of gauze covered with Micropore tape, and the trunk was loosely bound with Elastoplast Elastic Bandage


REMOVAL OF TEST SUBSTANCE
- Washing: with water dampened tissues
- Time after start of exposure: 4 hours


SCORING SYSTEM: OECD recommended scoring system

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

24/48/72 h

Score:

0.11

Max. score:

1

Reversibility:

fully reversible within: 16 days

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Reversibility:

other: not applicable

Irritant / corrosive response data:

Slight erythema was noted in one rabbit 72 h and 7 days after patch removal. This reaction had fully reversed by 16 days after patch removal. No other reactions were noted.

Any other information on results incl. tables

Skin Reaction Scores:

Rabbit No.	Erythema						Edema
	1 h	24 h	48 h	72 h	7 d	16 d	1 h	24 h	48 h	72 h	7 d	16 d
1	0	0	0	0	0	0	0	0	0	0	0	0
2	0	0	0	0	0	0	0	0	0	0	0	0
3	0	0	0	1	1	0	0	0	0	0	0	0

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information

Conclusions:

Fluorobenzene is practically non-irritationg to rabbit skin.