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EC number: 202-338-6
CAS number: 94-49-5
Respiration rates of the activated sludge,
inhibition percentages at various concentrations of ethylene glycol
dibenzoate after 3 hours contact time.
In order to predict effects of chemicals in
the environment and in biological waste water treatment plants, an
activated sludge respiration inhibition test was performed. The toxicity
to activated sludge was determined in accordance with OECD Test
Guideline 209, and in compliance with the OECD principles of Good
The test is valid as shown by the EC50 of
the reference compound 3,5-dichlorophenol (10 mg/L) and the coefficient
of variation of the replicates of the control oxygen uptake rates (7%).
The average oxygen uptake rate for the control replicates is lower (16
mg O2/g dry weight/hour) than the prescribed rate (>20 mg O2/g dry
weight /hour). This does not influence the sensitivity of the activated
sludge as shown by comparable EC50 values of the reference substance
3,5-dichlorophenol at oxygen uptake rates lower and higher than the
prescribed rate (AkzoNobel 2012). The lower average oxygen uptake rate
not compromise the test outcome in terms of
robustness of the endpoints obtained for the test and reference
substance and does not invalidate the test.
The toxicity of ethylene glycol dibenzoate
to activated sludge was determined at a contact time of 3 hours, using
various concentrations of the test substance. The inhibitory effect of
ethylene glycol dibenzoate at a particular concentration is expressed as
a percentage of the two controls. From the results EC values were
calculated. The EC10, EC20, EC50 and EC80 values of ethylene glycol
dibenzoate for activated sludge after 3 hours contact time are all >1280
mg/L , the highest concentration tested. Ethylene glycol dibenzoate is
therefore considered not harmful to activated sludge.
The toxicity to activated sludge was
determined in accordance with OECD Test Guideline 209, and in compliance
with the OECD principles of Good Laboratory Practice.
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