Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-338-6
CAS number: 94-49-5
A study was performed to assess the chronic
toxicity of the test item to Daphnia magna. The method followed
was designed to be compatible with the OECD Guidelines for Testing of
Chemicals (2012) No 211, "Daphniamagna Reproduction Test"
referenced as Method C.20 of Commission Regulation (EC) No. 440/2008.
Preliminary solubility work conducted
indicated that it was not possible to obtain a testable solution of the
test item using traditional methods of preparation e.g. ultrasonication
and high shear mixing.
A preliminary media preparation trial
indicated that a saturated solution method of preparation was most
appropriate for this test item.
Based on the results of a preliminary
range‑finding test, Daphnia magna were exposed
(10 replicates of a single daphnid per group) to solutions of the test
item at nominal concentrations of 6.25, 12.5, 25, 50 and 100% v/v
saturated solution for a period of 21 days. The test solutions were
renewed daily throughout the test. The test item solutions were
prepared by stirring an excess (100 mg/L) of test item in culture medium
using a propeller stirrer at approximately 1500 rpm for 48 hours. After
the stirring period any undissolved test item was removed by filtration
(0.2 µm Sartorius Sartopore filter, first approximate 2 liters discarded
in order to pre‑condition the filter) to produce a 100% v/v saturated
solution of the test item. This saturated solution was then further
diluted as necessary, to provide the remaining test groups.
The numbers of live and dead adult daphnia
and young daphnids (live and dead) were determined daily. The daphnia
were fed daily with a mixture of algal suspension and Tetramin®flake
Analysis of the fresh 50 and 100% v/v
saturated solution test preparations on Days 0, 5, 12 and 21 showed
measured test concentrations to range from 0.56 to 2.0 mg/L. A decline
in measured test concentration of the aged test preparations on Days 1,
6, 13 and 21 was observed to between 0.31 and 1.6 mg/Land hence it was
considered appropriate to calculate the results based on the geometric
mean measured test concentrations only in order to give a “worst case”
analysis of the data. The geometric mean measured concentrations were
0.65 and 1.4 mg/L.
Exposure of Daphnia magna to the test
item gave the following results based on the geometric mean measured
Geometric Mean Measured Concentration (mg/L)
95% confidence limits
0.73 – 0.84
Not possible to determine due to the nature of the data
No Observed Effect Concentration
Lowest Observed Effect Concentration
long-term study according to OECD 211 guideline under GLP was conducted
with Daphnia magna.
test was performed with a semi-static system, and test solutions were
geometric mean exposure concentration were calculated and used for
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again