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EC number: 458-890-5 | CAS number: 2138848-94-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Meets the criteria for classification as Reliable without restriction according to Klimisch et al (1997)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 458-890-5
- EC Name:
- -
- Cas Number:
- 2138848-94-7
- Molecular formula:
- C35H31N8O18S3.xNa.yK, where x+y=3
- IUPAC Name:
- dipotassium sodium 3-[(1E)-2-{4-[(1E)-2-{4-[(1E)-2-(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl)diazen-1-yl]-2-sulfonatophenyl}diazen-1-yl]-2,5-bis(2-hydroxyethoxy)phenyl}diazen-1-yl]-4,5-dihydroxynaphthalene-2,7-disulfonate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The concentrations of test substance in the test solutions were measured at 0 and 96 hours using the high performance liquid chromatography.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- This study was run with a single nominal concentration of 120 mg/l of test substance together with a dilution water control. The test solution was prepared by the addition of a known amount of test substance directly to 20 litres of dilution water in the test vessel, resulting in a dark blue/black solution.
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- The test organism was the rainbow trout Oncorhynchus mykiss. The fish (batch 04/04) were obtained from Houghton Springs Fish Farm, Winterbourne Houghton, Blandford Forum, Dorset, DT11 OPD, UK on 20 January 2004 and held in stock tanks at Brixham Environmental Laboratory for at least 12 days before the start of the test. Prior to the test the fish were acc1imatised to the test temperature (15 ± 1°C) for a minimum period of 7 days.
The last medication given to the fish was a 10 day course of amoxycyllin. The treatment was completed on 9 February 2004, 14 days prior to the test. No mortality was observed in the batch of fish in the 7 days prior to the test.
During the holding and acc1imatisation periods the fish were fed appropriate amounts of a commercial fish food. Food was withheld from the fish for at least 24 hours prior to the commencement of the test.
At the end of the exposure period, the fish from the dilution water control were weighed and measured. The range in weight was 1.31 to 2.60 g with a mean of 1.73 g, and the range in length was 45 to 54 mm with a mean of 48 mm.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- The total hardness of the dilution water was 49.3 mg/l CaC03, and the conductivity of the water was 228 uS cm-I @ 25°C.
. - Test temperature:
- Test was undertaken in a temperature controlled room. Test temperature was recorded continuously was within the range 15 ± 1°C.
- pH:
- pH values were recorded at 0, 24, 48, 72 and 96 hours and ranged from 7.3 to 7.7
- Dissolved oxygen:
- At no time during the course of the study was dissolved oxygen concentration in any of the test vessels less than 60% of the air-saturation value as given in the: National Institute of Oceanography of Great Britain and UNESCO (1973). International Oceanographic Tables, Vol 2
- Salinity:
- Not applicable - freshwater test
- Nominal and measured concentrations:
- The overall mean measured concentration was 100% of the nominal value. The percentage loss in the measured concentration over the test period was <1%.
The nominal concentrations were used for the calculation and reporting of the results. - Details on test conditions:
- The test procedure employed was a static system. Single borosilicate glass vessels (external dimensions; 400 mm x 280 mm x 280 mm; length x width x height) were used for the dilution water control and the exposure solution. The vessels had a working volume of 20 litres.
The test was undertaken in a temperature controlled room which was set at the nominal test temperature of 15 ± 1°C. The test solutions were gently aerated. The photoperiod in this study was 16 hours fluorescent light and 8 hours dark with 20 minute dawn and dusk transition periods commencing at 06:00 and 21:40 hours. At the start of the test ten fish were randomly allocated to the single test concentration and the dilution water control. The fish were not fed during the course of the test. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 120 mg/L
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 120 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance showed no toxicity to fish under the test conditions.
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