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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Study period:
Jan 1970
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Documentation of composition/type of substance insufficient for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1973
Report date:
1973

Materials and methods

Principles of method if other than guideline:
Method: single intravenous injection, test substance injected in physiological saline, 4 dose groups, 15 rats/group, observation time 7 days, determination of signs of intoxication, calculation of LD50
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Paraffin oils, sulfochlorinated, saponified
EC Number:
269-144-1
EC Name:
Paraffin oils, sulfochlorinated, saponified
Cas Number:
68188-18-1
Molecular formula:
typical example: C15H31Na03S
IUPAC Name:
n-C14-C17 alkanes, secondary monosulphonic acids, sodium salts
Details on test material:
purity not specified
Specific details on test material used for the study:
Information from study report:
Mersolat-Paste (pH 8.25), technical grade

Test animals

Species:
mouse
Strain:
NMRI
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Winkelmann, Kirchborchen, Kreis Paderborn, Germany
- Weight at study initiation: 18-22 g

Administration / exposure

Route of administration:
intravenous
Vehicle:
physiological saline
Details on exposure:
INJECTION VELOCITY: 1 mL/min
Doses:
100, 150, 200, 250, and 350 mg/kg bw
No. of animals per sex per dose:
15 males
Details on study design:
- Duration of observation period following administration: 7 days
- Determination of signs of intoxication
Statistics:
Calculation of LD50 according to Litchfield and Wilcoxon (J. Pharmacol. Exper. Therap. 96, 99, 1949)

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
195 mg/kg bw
95% CL:
173.1 - 219.6
Mortality:
Mortalities in dose groups: 100 mg/kg 0/15, 150 mg/kg 1/15, 200 mg/kg 8/15, 250 mg/kg 14/15, and 350 mg/kg 15/15. Deaths occurred immediately after injection.
Minimum lethal dose: 150 mg/kg
Clinical signs:
Animals were severly affected 1 to 10 minutes after injection. Typical signs of intoxication could not be noted. After 3 to 5 hours the animals returned to normal.
Body weight:
no data
Gross pathology:
no data

Applicant's summary and conclusion

Executive summary:

An LD50 -test was conducted with groups of male mice receiving a single intravenous administration of the test substance at doses of 100 to 350 mg/kg bw in ohysiological saline. The observation time was 7 days. Deaths occurred immediately after injection. The animals were severly affected 1 to 10 minutes after injection. Typical signs of intoxication could not be noted. Surviving animals returned to normal 3 to 5 hours post administration. The LD50 was calculated to be 195 mg/kg bw (95 % confidence level 173.1 -219.6 mg/kg).