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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991-03-11 till 1991-04-13
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
SAMPLE COLLECTION
The samples were collected by staff members of the ecotoxicology group. The samples were transferred into amber hottles and stored until analysis at below -20°C.

HANDLING
Analyses were performed in duplicate, no sample was stored.

Vehicle:
no
Details on test solutions:
- Date of purchase: March 26, 1991
- Size of stock vessel: 182 L; height: 49 cm; length: 98 cm; with: 38 cm Material of stock vessel: glass
- Amount of water per stock vessel: 149 L
- Depth of water in the stock vessel: approximately 40 cm
- Estimated number of fish kept as a stock per holding vessel (batchsize): .200
- Water: reconstituted water
- Renewal of water: Flow through - weekly turnover rate: approximately 1000-1500 L Temperature of water: 20/22 min/max °C
- Oxygen content of water: 80/100 min/max % 8.5/9.5 min/max mg/L
- Hardness of water: 150/250 min/max mg CaCO3/L
Under holding conditions the hardness of water is measured infrequently. The lowest and highest value ever measured is given above. However, themore realistic values correspond to those measured for the reconstituted water in this study since the reconstituted water for the holding of fish
and the test were derived from the same source.

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- Test Species: Brachydanio rerio (Hamilton Buchanan), Zebra fish
- Supplier: Zoozentrum Höchst, Frankfurt a.M.
- Starting Date (in-life phase): 1991-03-11
- Completion Date (in-life phase): 1991-04-13
- Average body weight of fish (sample consisting of 10 fish): 0.209 ± 0.027 (S.D.) g/fish Loading: 0.281 g/L
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
8.4 - 14.0°dH (150 - 250 mg CaCO3/L)
Test temperature:
20 -22 °C
pH:
7.5 - 7.9
Dissolved oxygen:
Oxygen content of water: 8.5/9.5 mg/L (80/100 %)
Nominal and measured concentrations:
Nominal concentration of the stock solution: 101.5 mg/L
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
4.16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Test concentration was analytically confirmed
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
2.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Test concentration was analytically confirmed
Duration:
96 h
Dose descriptor:
LOEC
Effect conc.:
5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Test concentration was analytically confirmed
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
0.66 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Based on measured concentration at the end of the test
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Based on measured concentration at the end of the test
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
2.65 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Based on measured concentration at the end of the test
Details on results:
- The analytically determined actual concentrations of the test material in the test solutions at the beginning of the study were found to be within the range of 109 % and 143 % with an average of 121.3 %.
- The analytically determined stability of the test material in the test solutions after 96 hours of exposure was found to be within the range of 13 % and 42 % of the nominal values with an average of 26.5 %.

Reported statistics and error estimates:
Probit Analysis (Finney 1971) was used to determine LC-values. When less than 3 test substance concentrations caused effects between O and 100 % then the geometrical mean was applied.

MBAS Content of the Product

 Nominal Amount of Emulgator E 30 fest  Extinction E650  µg MBAS determined
 2000 µg  n.d.  n.d.
 1000 µg  3.5  682.1
 500 µg  1.025  199.8
 200 µg  0.661  128.8

n.d. = not determinable because the values were out of range

Validity criteria fulfilled:
yes
Conclusions:
The acute toxicity of Paraffin oils, sulfochlorinated, saponified to Fish (Danio rerio) was tested according to OECD Guideline no. 203 "Fish, Acute Toxicity Test, adopted 1984". The lethal concetration LC50 was determined during 96 hours to be 1.10 mg/L (Leuna, AG, 1991).
Executive summary:

In accordance with GLP regulations the acute toxicity of Emulgator E 30 to the fish Brachydanio rerio (Hamilton Buchanan), Zebra fish was investigated in a static test system during a test period of 96 hours. The study was performed according to the OECD Guideline 203, 1984.

The nominal concentrations to which the test organisms were exposed were 0.625, 1.25, 2.5, 5.0 and 10.0 mg/L Emulgator E 30.

The analytically determined actual concentrations of the test material in the test solutions at the beginning of the study were found to be within the range of 109% and 143% with an average of 121.3%.

Based on nominal concentrations the study resulted in the following value:

LC50 (96 h): 4.16 mg/L Emulgator E 30

No Observed Effect Concentration (NOEC): 2.50 mg/L Emulgator E 30

Based on actual (measured) concentrations at the end of the test ( on average 26.5% nominal) the study resulted in the following value:

LC50 (96 h): 1.10 mg/L Emulgator E 30

No Observed Effect Concentration (NOEC): 0.66 mg/L Emulgator E 30

Description of key information

The acute toxicity of Paraffin oils, sulfochlorinated, saponified to Fish (Danio rerio) was tested according to OECD Guideline no. 203 "Fish, Acute Toxicity Test, adopted 1984". The lethal concentration LC50 was determined during 96 hours to be 1.10 mg/L (Leuna, AG 1991).

Key value for chemical safety assessment

LC50 for freshwater fish:
1.1 mg/L

Additional information

Result based on actual (measured) concentrations at the end of the test (on average 26.5% nominal)