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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guideline study with acceptable restrictions.
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
-Melting point: 63°C
-Boiling point: 205°C
-Purity: 99.0 %
-Lot no.: 08826KS
-Storage conditions and stability were checked and measured using infrared spectroscopy
Analytical monitoring:
yes
Details on sampling:
Concentration of 100 mg/L was measured at 0 and 72 hours
Vehicle:
no
Details on test solutions:
OECD Medium used
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
This species is obtained from the American Type Culture Collection ATCC 22662
Test type:
static
Water media type:
freshwater
Total exposure duration:
72 h
Test temperature:
23 °C +/- 2 °C (0-72h)
pH:
7.9 - 8.1 (0-72h)
Nominal and measured concentrations:
Nominal concentrations of the test substance: 0.05, 0.10, 0.20, 0.40, 0,80, 1.60 mg/L
Measured concentration of the test substance (geometric mean): 0.016, 0.038, 0.079, 0.133, 0.188, 0.137 mg/L (0-24h)
Details on test conditions:
Cultivation system: shaking (100 rpm)
Test duration: 72 hours
cell concentration: 1 x 10^4 cells/mL
test volume: 100 mL
temperature: 23. 0 +/- 2.0°C
lighting: 4440-8880 lux, in a continuous illumination (light constant during the study period).
pH: during the study period, not adjusted
Reference substance (positive control):
no
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.477 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: ErC50
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: NOErC
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.177 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: EbC50
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
< 0.05 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: NOEbC
Details on results:
ErC50 and EbC50 determined via Probit Method
NOErC and NOEbC determined via Dunnett Method
Reported statistics and error estimates:
ErC50 and NOErC values: 95% confidence interval 0.441 - 0.515 mg/L
EbC50 and NOEbC values: 95% confidence interval 0.162 - 0.193 mg/L
Validity criteria fulfilled:
yes
Remarks:
Factor of biomass parameter > 16. The mean of the replicate coefficents of variation in the section-by section growth 9 % < 35 %. The coefficient of variation of the mean specific growth rate replictes in the control < 7 %
Conclusions:
The acute toxicity to algae ( Pseudokircheneriella subcapitata) was determined during 72 hours exposure and showed an EC50 of 0.48 mg/L and a NOEC of 0.10 mg/L was calculated.
Executive summary:

The acute toxicity to algae (Pseudokircheneriella subcapitata) was determined acoording OECD TG 201'Algal growth inhibition Test' and

Pseudokircheneriella subcapitata has been exposed to a range of concentrations (0.05, 0.1, 0.5, 1.0, 2.5, 5.0 mg/L). After an exposure of

72 hours an EC50 of 0.48 mg/L and an EbC50 of 0.18 mg/L was determined. A NOEC of 0.10 mg/L and a NOEbC value of <0.05 mg/L were calculated for 1,8-napthylenediamine.

Description of key information

The acute toxicity to algae (Pseudokircheneriella subcapitata) was determined acoording OECD TG 201'Algal growth inhibition Test' and

Pseudokircheneriella subcapitata has been exposed to a range of concentrations (0.05, 0.1, 0.5, 1.0, 2.5, 5.0 mg/L). After an exposure of

72 hours an EC50 of 0.48 mg/L and an EbC50 of 0.18 mg/L was determined. A NOEC of 0.10 mg/L and a NOEbC value of <0.05 mg/L were calculated for 1,8-napthylenediamine.

Key value for chemical safety assessment

EC50 for freshwater algae:
0.48 mg/L
EC10 or NOEC for freshwater algae:
0.1 mg/L

Additional information